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- PDB-8ahb: rsEGFP2 photoswitched to its off-state at room temperature and ba... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8ahb | ||||||
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Title | rsEGFP2 photoswitched to its off-state at room temperature and back-switched to its on-state at 100K | ||||||
![]() | Green fluorescent protein | ||||||
![]() | FLUORESCENT PROTEIN / RSFP / Switchable / PTFP / rsEGFP2 | ||||||
Function / homology | Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / generation of precursor metabolites and energy / Green fluorescent protein![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Mantovanelli, A. / Adam, V. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Photophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2. Authors: Mantovanelli, A.M.R. / Glushonkov, O. / Adam, V. / Wulffele, J. / Thedie, D. / Byrdin, M. / Gregor, I. / Nevskyi, O. / Enderlein, J. / Bourgeois, D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 78.4 KB | Display | ![]() |
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PDB format | ![]() | 45.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 444.3 KB | Display | ![]() |
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Full document | ![]() | 445.5 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 15.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ahaC ![]() 5dtyS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28532.203 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.46 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.1 / Details: 100 mM HEPES buffer, pH 8.1 1.9 M ammonium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Feb 18, 2022 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 1.79→46.44 Å / Num. obs: 254080 / % possible obs: 99.36 % / Redundancy: 11.8 % / Biso Wilson estimate: 34.59 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.1268 / Rpim(I) all: 0.03823 / Net I/σ(I): 10.87 |
Reflection shell | Resolution: 1.79→1.858 Å / Redundancy: 7.3 % / Mean I/σ(I) obs: 0.45 / Num. unique obs: 14673 / CC1/2: 0.237 / CC star: 0.619 / % possible all: 94.43 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5DTY Resolution: 1.79→46.44 Å / SU ML: 0.3616 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.8556 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.37 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.79→46.44 Å
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Refine LS restraints |
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LS refinement shell |
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