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TitlePhotophysical Studies at Cryogenic Temperature Reveal a Novel Photoswitching Mechanism of rsEGFP2.
Journal, issue, pagesJ. Am. Chem. Soc., Vol. 145, Page 14636-14646, Year 2023
Publish dateJul 21, 2022 (structure data deposition date)
AuthorsMantovanelli, A.M.R. / Glushonkov, O. / Adam, V. / Wulffele, J. / Thedie, D. / Byrdin, M. / Gregor, I. / Nevskyi, O. / Enderlein, J. / Bourgeois, D.
External linksJ. Am. Chem. Soc. / PubMed:37389576
MethodsX-ray diffraction
Resolution1.79 - 2.38 Å
Structure data

PDB-8aha:
rsEGFP2 photoswitched to its off-state at 100K
Method: X-RAY DIFFRACTION / Resolution: 2.38 Å

PDB-8ahb:
rsEGFP2 photoswitched to its off-state at room temperature and back-switched to its on-state at 100K
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

Source
  • aequorea victoria (jellyfish)
KeywordsFLUORESCENT PROTEIN / RSFP / Switchable / PTFP / rsEGFP2

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