ジャーナル: Sci Adv / 年: 2023 タイトル: BRCA2-HSF2BP oligomeric ring disassembly by BRME1 promotes homologous recombination. 著者: Rania Ghouil / Simona Miron / Koichi Sato / Dejan Ristic / Sari E van Rossum-Fikkert / Pierre Legrand / Malika Ouldali / Jean-Marie Winter / Virginie Ropars / Gabriel David / Ana-Andreea ...著者: Rania Ghouil / Simona Miron / Koichi Sato / Dejan Ristic / Sari E van Rossum-Fikkert / Pierre Legrand / Malika Ouldali / Jean-Marie Winter / Virginie Ropars / Gabriel David / Ana-Andreea Arteni / Claire Wyman / Puck Knipscheer / Roland Kanaar / Alex N Zelensky / Sophie Zinn-Justin / 要旨: In meiotic homologous recombination (HR), BRCA2 facilitates loading of the recombinases RAD51 and DMC1 at the sites of double-strand breaks (DSBs). The HSF2BP-BRME1 complex interacts with BRCA2. Its ...In meiotic homologous recombination (HR), BRCA2 facilitates loading of the recombinases RAD51 and DMC1 at the sites of double-strand breaks (DSBs). The HSF2BP-BRME1 complex interacts with BRCA2. Its absence causes a severe reduction in recombinase loading at meiotic DSB. We previously showed that, in somatic cancer cells ectopically producing HSF2BP, DNA damage can trigger HSF2BP-dependent degradation of BRCA2, which prevents HR. Here, we report that, upon binding to BRCA2, HSF2BP forms octameric rings that are able to interlock into a large ring-shaped 24-mer. Addition of BRME1 leads to dissociation of both of these ring structures and cancels the disruptive effect of HSF2BP on cancer cell resistance to DNA damage. It also prevents BRCA2 degradation during interstrand DNA crosslink repair in egg extracts. We propose that, during meiosis, the control of HSF2BPBRCA2 oligomerization by BRME1 ensures timely assembly of the ring complex that concentrates BRCA2 and controls its turnover, thus promoting HR.
構造決定の手法: 分子置換 開始モデル: AlphaFold2 model 解像度: 1.484→56.43 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.95 / SU R Cruickshank DPI: 0.087 / 交差検証法: THROUGHOUT / SU R Blow DPI: 0.083 / SU Rfree Blow DPI: 0.075 / SU Rfree Cruickshank DPI: 0.071
Rfactor
反射数
%反射
Selection details
Rfree
0.2166
664
-
RANDOM
Rwork
0.2127
-
-
-
obs
0.2129
14159
94.7 %
-
原子変位パラメータ
Biso mean: 35.8 Å2
Baniso -1
Baniso -2
Baniso -3
1-
0 Å2
0 Å2
0 Å2
2-
-
0 Å2
0 Å2
3-
-
-
0 Å2
Refine analyze
Luzzati coordinate error obs: 0.22 Å
精密化ステップ
サイクル: LAST / 解像度: 1.484→56.43 Å
タンパク質
核酸
リガンド
溶媒
全体
原子数
506
0
10
56
572
拘束条件
Refine-ID
タイプ
Dev ideal
数
Restraint function
Weight
X-RAY DIFFRACTION
t_bond_d
0.008
1276
HARMONIC
2
X-RAY DIFFRACTION
t_angle_deg
0.94
2332
HARMONIC
2
X-RAY DIFFRACTION
t_dihedral_angle_d
407
SINUSOIDAL
2
X-RAY DIFFRACTION
t_gen_planes
191
HARMONIC
5
X-RAY DIFFRACTION
t_it
1276
HARMONIC
10
X-RAY DIFFRACTION
t_chiral_improper_torsion
78
SEMIHARMONIC
5
X-RAY DIFFRACTION
t_sum_occupancies
9
HARMONIC
1
X-RAY DIFFRACTION
t_ideal_dist_contact
1219
SEMIHARMONIC
4
X-RAY DIFFRACTION
t_omega_torsion
2.94
X-RAY DIFFRACTION
t_other_torsion
13.42
LS精密化 シェル
解像度: 1.484→1.52 Å
Rfactor
反射数
%反射
Rfree
0.2707
19
-
Rwork
0.2343
-
-
obs
-
-
46.36 %
精密化 TLS
Origin x: -10.747 Å / Origin y: 3.7246 Å / Origin z: -15.8911 Å