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Open data
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Basic information
Entry | Database: PDB / ID: 7zs3 | ||||||
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Title | Crystal structure of poplar glutathione transferase U19 | ||||||
![]() | Glutathione transferase | ||||||
![]() | TRANSFERASE / Glutathione / Tau | ||||||
Function / homology | ![]() response to chemical / glutathione transferase / glutathione transferase activity / glutathione metabolic process / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Didierjean, C. / Favier, F. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Biochemical and Structural Insights on the Poplar Tau Glutathione Transferase GSTU19 and 20 Paralogs Binding Flavonoids. Authors: Sylvestre-Gonon, E. / Morette, L. / Viloria, M. / Mathiot, S. / Boutilliat, A. / Favier, F. / Rouhier, N. / Didierjean, C. / Hecker, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 65.6 KB | Display | ![]() |
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PDB format | ![]() | 47 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.5 KB | Display | ![]() |
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Full document | ![]() | 434.4 KB | Display | |
Data in XML | ![]() | 12.3 KB | Display | |
Data in CIF | ![]() | 17.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7zvpC ![]() 7zznC ![]() 8a08C ![]() 8a0iC ![]() 8a0oC ![]() 8a0pC ![]() 8a0qC ![]() 8a0rC ![]() 4j2fS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 25053.969 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||||
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#2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58.09 % |
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Crystal grow | Temperature: 277 K / Method: microbatch / pH: 6.5 Details: 0.2 M Calcium acetate hydrate, 0.1 M Sodium cacodylate pH 6.5 and 18 % w/v PEG 8000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 23, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97857 Å / Relative weight: 1 |
Reflection | Resolution: 1.603→41.5 Å / Num. obs: 40239 / % possible obs: 99.7 % / Redundancy: 18 % / CC1/2: 1 / Rmerge(I) obs: 0.068 / Rrim(I) all: 0.07 / Net I/σ(I): 22.5 |
Reflection shell | Resolution: 1.603→1.63 Å / Num. unique obs: 1870 / CC1/2: 0.8 / % possible all: 95.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4J2F Resolution: 1.603→16.11 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.951 / SU R Cruickshank DPI: 0.094 / Cross valid method: THROUGHOUT / SU R Blow DPI: 0.101 / SU Rfree Blow DPI: 0.091 / SU Rfree Cruickshank DPI: 0.087
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Displacement parameters | Biso mean: 35.74 Å2
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Refine analyze | Luzzati coordinate error obs: 0.25 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.603→16.11 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.603→1.62 Å
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