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- PDB-7zju: Structure of human USPL1 in covalent complex with SUMO3-2Br probe -

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Basic information

Entry
Database: PDB / ID: 7zju
TitleStructure of human USPL1 in covalent complex with SUMO3-2Br probe
Components
  • SUMO-specific isopeptidase USPL1
  • Small ubiquitin-related modifier 3
KeywordsHYDROLASE / USP / SUMO / Ubiquitin / probe / ubiquitin-like modifier
Function / homology
Function and homology information


Cajal body organization / snRNA transcription / deSUMOylase activity / SUMO is proteolytically processed / SUMO is conjugated to E1 (UBA2:SAE1) / protein desumoylation / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / SUMO binding / SUMOylation of immune response proteins / regulation of protein localization to nucleus ...Cajal body organization / snRNA transcription / deSUMOylase activity / SUMO is proteolytically processed / SUMO is conjugated to E1 (UBA2:SAE1) / protein desumoylation / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / SUMO binding / SUMOylation of immune response proteins / regulation of protein localization to nucleus / negative regulation of DNA binding / ubiquitin-like protein ligase binding / SUMOylation of DNA replication proteins / protein sumoylation / SUMOylation of transcription factors / Cajal body / SUMOylation of DNA damage response and repair proteins / SUMOylation of chromatin organization proteins / SUMOylation of transcription cofactors / SUMOylation of intracellular receptors / PML body / kinetochore / protein tag activity / Formation of Incision Complex in GG-NER / cell population proliferation / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / proteolysis / extracellular space / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
Ubiquitin-specific peptidase-like, SUMO isopeptidase / Domain of unknown function DUF4650 / USPL1 peptidase / Ubiquitin-specific peptidase-like, SUMO isopeptidase / Domain of unknown function (DUF4650) / Rad60/SUMO-like domain / Ubiquitin-2 like Rad60 SUMO-like / Ubiquitin specific protease domain / Ubiquitin specific protease (USP) domain profile. / Papain-like cysteine peptidase superfamily ...Ubiquitin-specific peptidase-like, SUMO isopeptidase / Domain of unknown function DUF4650 / USPL1 peptidase / Ubiquitin-specific peptidase-like, SUMO isopeptidase / Domain of unknown function (DUF4650) / Rad60/SUMO-like domain / Ubiquitin-2 like Rad60 SUMO-like / Ubiquitin specific protease domain / Ubiquitin specific protease (USP) domain profile. / Papain-like cysteine peptidase superfamily / Ubiquitin homologues / Ubiquitin-like domain / Ubiquitin domain profile. / Ubiquitin-like domain superfamily
Similarity search - Domain/homology
2-bromanylethanamine / Small ubiquitin-related modifier 3 / SUMO-specific isopeptidase USPL1
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.17 Å
AuthorsZhao, Z. / Gersch, M.
Funding support Germany, 1items
OrganizationGrant numberCountry
Max Planck Society Germany
Citation
Journal: J.Am.Chem.Soc. / Year: 2023
Title: Native Semisynthesis of Isopeptide-Linked Substrates for Specificity Analysis of Deubiquitinases and Ubl Proteases.
Authors: Zhao, Z. / O'Dea, R. / Wendrich, K. / Kazi, N. / Gersch, M.
#1: Journal: Acta Crystallogr.,Sect.D / Year: 2012
Title: Towards automated crystallographic structure refinement with phenix.refine.
Authors: Afonine, P.V. / Grosse-Kunstleve, R.W. / Echols, N. / Headd, J.J. / Moriarty, N.W. / Mustyakimov, M. / Terwilliger, T.C. / Urzhumtsev, A. / Zwart, P.H. / Adams, P.D.
#2: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionApr 12, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 27, 2023Provider: repository / Type: Initial release
Revision 1.1Oct 4, 2023Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.name
Revision 1.2Oct 11, 2023Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.3Nov 15, 2023Group: Database references / Category: citation_author / Item: _citation_author.identifier_ORCID

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: SUMO-specific isopeptidase USPL1
B: Small ubiquitin-related modifier 3
C: SUMO-specific isopeptidase USPL1
D: Small ubiquitin-related modifier 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)87,91515
Polymers87,1094
Non-polymers80611
Water5,963331
1
A: SUMO-specific isopeptidase USPL1
B: Small ubiquitin-related modifier 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)43,9127
Polymers43,5552
Non-polymers3575
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: SUMO-specific isopeptidase USPL1
D: Small ubiquitin-related modifier 3
hetero molecules


Theoretical massNumber of molelcules
Total (without water)44,0048
Polymers43,5552
Non-polymers4496
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)66.060, 84.833, 71.106
Angle α, β, γ (deg.)90.000, 92.257, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z

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Components

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Protein , 2 types, 4 molecules ACBD

#1: Protein SUMO-specific isopeptidase USPL1


Mass: 33203.062 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: USPL1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q5W0Q7
#2: Protein Small ubiquitin-related modifier 3 / SUMO-3 / SMT3 homolog 1 / SUMO-2 / Ubiquitin-like protein SMT3A / Smt3A


Mass: 10351.587 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: SUMO3, SMT3A, SMT3H1 / Production host: Escherichia coli (E. coli) / References: UniProt: P55854

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Non-polymers , 6 types, 342 molecules

#3: Chemical ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Zn
#4: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C3H8O3
#5: Chemical ChemComp-OXU / 2-bromanylethanamine


Mass: 123.980 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6BrN / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#7: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Ca
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 331 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.29 Å3/Da / Density % sol: 46.18 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.8 / Details: 100 mM Tris pH 8.8, 19% PEG6000, 200 mM CaCl2

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 1 Å
DetectorType: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Jun 22, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.17→54.47 Å / Num. obs: 80896 / % possible obs: 99.9 % / Redundancy: 5.7 % / Biso Wilson estimate: 33.81 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.112 / Rrim(I) all: 0.123 / Net I/σ(I): 8.5
Reflection shellResolution: 2.17→2.25 Å / Redundancy: 5 % / Rmerge(I) obs: 0.64 / Mean I/σ(I) obs: 2.4 / Num. unique obs: 3592 / CC1/2: 0.887 / Rrim(I) all: 0.711 / % possible all: 99.6

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Processing

Software
NameVersionClassification
PHENIX1.18.2_3874refinement
xia20.5.902data reduction
DIALS1.14.13data collection
Aimless1.12.2data scaling
CRANK22.0.251phasing
RefinementMethod to determine structure: SAD / Resolution: 2.17→54.47 Å / SU ML: 0.2198 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 26.7196
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2378 4021 4.97 %
Rwork0.1919 76875 -
obs0.1942 80896 99.1 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 45.34 Å2
Refinement stepCycle: LAST / Resolution: 2.17→54.47 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5166 0 30 331 5527
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00345313
X-RAY DIFFRACTIONf_angle_d0.63987226
X-RAY DIFFRACTIONf_chiral_restr0.0465811
X-RAY DIFFRACTIONf_plane_restr0.0041928
X-RAY DIFFRACTIONf_dihedral_angle_d19.16321845
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.17-2.20.31821190.27862581X-RAY DIFFRACTION95.68
2.2-2.220.27121380.26762633X-RAY DIFFRACTION97.95
2.22-2.250.34851160.26282631X-RAY DIFFRACTION98.64
2.25-2.280.30251400.2522688X-RAY DIFFRACTION99.09
2.28-2.310.26971370.25742644X-RAY DIFFRACTION99.07
2.31-2.340.29111150.23762663X-RAY DIFFRACTION99
2.34-2.380.27791230.24112656X-RAY DIFFRACTION99.46
2.38-2.420.20751490.22842674X-RAY DIFFRACTION99.37
2.42-2.460.33611420.23072675X-RAY DIFFRACTION98.88
2.46-2.50.26961370.22362638X-RAY DIFFRACTION99.14
2.5-2.540.25531170.22942668X-RAY DIFFRACTION98.79
2.54-2.590.2518960.22242602X-RAY DIFFRACTION98.86
2.59-2.650.26541320.22072714X-RAY DIFFRACTION98.96
2.65-2.70.2571400.20932657X-RAY DIFFRACTION99.22
2.7-2.770.24791180.20972684X-RAY DIFFRACTION99.01
2.77-2.840.22921490.20962689X-RAY DIFFRACTION99.65
2.84-2.910.27261840.20852586X-RAY DIFFRACTION99.71
2.91-30.25311690.20962631X-RAY DIFFRACTION99.75
3-3.090.2161800.17912617X-RAY DIFFRACTION99.96
3.09-3.20.24891860.19342588X-RAY DIFFRACTION99.71
3.21-3.330.24711420.18312689X-RAY DIFFRACTION99.61
3.33-3.480.21881540.16812620X-RAY DIFFRACTION99.11
3.49-3.670.23761180.1592700X-RAY DIFFRACTION98.95
3.67-3.90.24271450.15852609X-RAY DIFFRACTION99.03
3.9-4.20.19951270.15682668X-RAY DIFFRACTION99.32
4.2-4.620.17711360.152679X-RAY DIFFRACTION99.58
4.62-5.290.2037700.15872742X-RAY DIFFRACTION100
5.29-6.660.23171560.20062654X-RAY DIFFRACTION99.86
6.66-54.470.23341860.21222595X-RAY DIFFRACTION98.79
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.531341977880.591448888702-1.174341564754.57816444206-0.5113960779025.268155709460.0795042998234-0.1337196601580.0009778449605750.286949926488-0.01679560223860.6813473367540.216255783405-0.310794369908-0.004609515038670.3029627426330.03410630304530.08430063570660.194890528412-0.02253981171950.29111459661725.855464692126.448295816533.1383103043
23.194033273321.728556896013.160200652915.247203955481.469568190733.23065670368-0.237659648856-0.2342107224820.6005829230960.638618372182-0.1337064290581.098074953730.0852658736279-0.6235429774510.3299097979380.6395102796160.06313472449870.2241651241540.4560731191430.01081228760020.52602006306319.234435255530.229274233737.6797157742
38.418838978923.571672261070.1886700220855.314107256371.463480450724.00988146250.473514473223-0.1361606994571.176365207850.675063963633-0.1968565578530.947718414895-0.527199369697-0.503266184794-0.141074735750.5901814362830.1007547678460.1542292099990.2463258338980.08584785007830.44400942568627.827945348336.561942715532.3109190053
42.614173623670.110632172087-0.01799503387064.74260722403-2.537833347666.95179198230.213352958248-0.049818166120.02514405777790.471303365540.2159754854330.203401552231-0.2361554471770.184029031384-0.4248667137710.260106397980.0233765061514-0.01190982245530.187235684217-0.0204883701010.19074415309638.646944263731.2472356231.1998426942
53.7674761659-2.27807135408-3.948911479213.584678409322.383324688474.60749260099-0.215973512318-0.7773679148550.3886996512810.999862775740.29168855803-0.4226745441750.06734519330810.4231643848990.04017837385710.613108538634-0.0576081356894-0.08557403982390.2712275694480.03169838258930.29693051322843.124426269116.36813898741.7396867906
68.31333331893-7.11121141293-6.192218424658.630795008233.498895969247.73785445809-0.0883404660969-0.555874496999-0.1415655480070.717380554419-0.159312651404-0.5664251446880.2667477295690.6057267120980.2613201671380.4943730482970.0244867768154-0.2079329560880.3544790554660.0181796190730.51245715880256.801937308710.205151694437.9461111573
74.55793102592-0.760542783467-0.1439289809412.762187970880.1137344006481.78839433052-0.1111705030760.271975193709-0.1913304614950.126995732867-0.023071520615-0.4507406435670.2327388577120.1428780653120.1349277423180.4176325201830.0496274318339-0.01297312427560.2488690914290.05936058475850.32767205949549.98908862168.7856331770429.3157166872
83.32378503867-0.423128742577-0.6949489480263.4694510473-0.9999628431313.223970485650.093586508403-0.0873324815591-0.2018162182210.500987261902-0.0447725337182-0.07600063402770.2094040793090.0585804379782-0.02347347015810.375652123805-0.00768221329849-0.006766561227240.1313059298690.03052225202040.21559019236141.661305403210.418214154532.6434939589
93.098924421571.04320588045-0.4540659271164.68431481732-1.936846880544.7850508670.216789968425-0.150936832128-0.2033438230170.588057744764-0.2294403357350.356796695860.310235160272-0.10623120953-0.02579084783730.413893334454-0.02975067571930.02433350996990.19214625203-0.02703190121630.1731569488132.70140714549.1962104278333.2109827683
107.958973917792.45949195597-2.475640792377.49887165281-3.12003329166.533900060230.1870960316230.4157501969020.349116496839-0.06257204277650.2947954465791.041957568580.282285535819-0.824927430005-0.3679695897990.40974324462-0.0259218394270.01004915279370.273105320483-0.03103852121620.26445225067525.666493288819.04783377123.8761074524
115.80468774753-0.1970839134880.3712843567613.61199847146-0.1170463853564.46651433570.05668574178330.1370146427090.0401080270286-0.170325467021-0.05282436879780.3936714860580.151997112868-0.3965429208010.02807833458690.395268567715-0.01617814736620.008963942704140.213656814859-0.0126906087130.29452684123527.696664184814.523766568326.6919519153
126.771404644383.834115615762.212642127599.237219062072.231006574414.733472852870.136392749228-0.07672438298690.1236106163290.02812976107-0.254882968913-0.8171455206370.004265388228980.5186355897220.09662260249320.280197922570.0120266396851-0.01594513027140.3197930541080.06661045015170.46214241460860.892910228627.673877789329.8807586878
131.754623414060.0773427092353-1.58843352884.09503397031-3.992001839427.678769347570.00752949075385-0.0508456104063-0.07299183426550.1072476541590.244857996582-0.2870777668680.482759584255-0.168348474292-0.04977933342560.3180065023430.0392040092423-0.06034013610480.3078486235870.03450270158250.36743112420251.909083295227.630651739722.3992678855
144.286616105084.914690505132.950904802456.001448654563.332777017832.38479920378-0.0258703977998-0.1694597925821.13243521015-0.296005635881-0.3530563541730.364905756031-0.2906119614210.04614610958850.3912336551210.60725032244-0.0834321011487-0.1089113756010.360685146134-0.0006821076305970.60866106136452.810861665535.868573925633.4589380957
153.999845624833.391327346745.149951308214.32149873852.686627340068.605679583610.0229949262673-0.0612944042809-0.2710662612780.577435577317-0.00514104145258-0.5570622917990.3161119937350.6496738610420.1774818630860.3939761511840.0389867078071-0.04218882590010.3541700235920.0579358308250.34085565677151.847855614127.250211998632.1392047793
163.7116983882-0.5659567672390.4366022654413.70555171579-0.6109917998632.47767521859-0.0005996840907270.308729433635-0.189073239278-0.4948651337030.08179686422960.4853426648940.125756217156-0.307165772277-0.08005562304870.33936794294-0.0117189997369-0.07849278715090.264259915364-0.0118971232410.27051174861627.788850797238.69699733111.10556027043
174.101277684690.3166659465180.6957188180592.8801714316-0.1720217503631.650878471860.02859214109210.2129926329080.129654743523-0.200677214746-0.036936607833-0.527549290871-0.1742598236980.2429666690020.01930401896930.444533489048-0.02955124926460.06738973576450.2386148409540.07746387956120.32482941689951.134777267155.65609992271.75817573564
183.54588035951-0.802651629430.103284773364.94506381866-1.929119706974.570573967170.159544807760.09019019945430.307558586612-0.1962968932290.1026664096030.359427787319-0.395576786335-0.468303054474-0.2696152742840.310829324440.07189224910980.006059860949170.265444915383-0.0009581737114460.21787483971131.404351406553.7820408138.09725989848
192.460371832110.965627253579-1.654702499274.729670535212.314580101043.115110533910.3142290821570.6449971240810.55087358708-1.52115858784-0.525084545879-1.65070473244-0.0129720184674-0.0719670495906-0.1075213809420.5020821612580.05030460000540.291539718140.3891344220930.1690887748710.76809366400862.083411977637.1650821472-0.678169929871
203.52452816646-3.663782044141.118352103435.166415508790.1911674453651.8287213888-0.02589122060110.355660812234-0.328638558987-0.329068785982-0.357057900108-0.933742736069-0.1976877806530.4113779764660.1050833619780.3510829189930.02410765419880.2596936658110.350911683950.1709976875420.86533880725461.430850768241.73117175343.62069636608
213.86938924422-1.24676140201-0.1506882331997.701557345592.881440460636.78712732717-0.123416038248-0.0859111462731-0.313444691946-0.0984968192757-0.0122156645838-0.9321262082930.7505092402270.3960210997720.03267767472430.366541006980.03496273843150.1221245999460.2993021720930.1179025501280.66831854726761.165341005832.20956254187.94935982732
220.9386487070171.97273885103-2.068995312635.54712471567-5.610998737586.291385372250.217479809482-0.329892491648-0.3355543617910.24797611456-0.214126119108-0.45034522358-0.60066537286-0.125888940935-0.1013046864530.2357243436980.01642381461350.09436503078580.3593028862220.02500733401790.4022113188652.554976801137.409225590812.5734796684
237.26719978967-5.92175724503-1.495480412248.864860983882.370196354592.1377906716-0.1099729423410.156093390731-1.13625359324-0.476795209216-0.375586769617-0.009352971203730.4194484898940.008171428320080.5105078045660.665855781614-0.03335546894760.2119666535490.38033308413-0.01723670339250.51399295547551.340790782230.26547928572.68180645337
242.86462420971-1.83247484966-0.04118117775117.569812306011.664327088042.46372387770.1574574943171.36017430118-0.05995569926-1.24222414187-0.229473150954-0.668261971920.2549676001520.1866509923820.1180769177410.6479862547910.1183843465980.2945706611930.607787894316-0.01824185791750.60087089454457.363389504630.2579986426-4.90253397426
257.86053439212-5.297347788-7.231912064526.032256989653.946245580826.984278354210.2156763030120.1197302829020.658201599048-0.364898879720.299483498462-0.5634469880520.006349294642420.0164601802471-0.309742982130.318484176342-0.0192683163367-0.01744560867710.280803209510.05377224464090.33852369079649.205642677740.50469977358.06837771369
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 225 through 264 )AA225 - 2641 - 35
22chain 'A' and (resid 265 through 301 )AA265 - 30136 - 51
33chain 'A' and (resid 302 through 323 )AA302 - 32352 - 73
44chain 'A' and (resid 324 through 343 )AA324 - 34374 - 93
55chain 'A' and (resid 344 through 360 )AA344 - 36094 - 110
66chain 'A' and (resid 361 through 374 )AA361 - 374111 - 124
77chain 'A' and (resid 375 through 404 )AA375 - 404125 - 154
88chain 'A' and (resid 405 through 430 )AA405 - 430155 - 180
99chain 'A' and (resid 431 through 452 )AA431 - 452181 - 202
1010chain 'A' and (resid 453 through 475 )AA453 - 475203 - 225
1111chain 'A' and (resid 476 through 501 )AA476 - 501226 - 251
1212chain 'B' and (resid 17 through 50 )BD17 - 502 - 35
1313chain 'B' and (resid 51 through 60 )BD51 - 6036 - 45
1414chain 'B' and (resid 61 through 75 )BD61 - 7546 - 60
1515chain 'B' and (resid 76 through 91 )BD76 - 9161 - 76
1616chain 'C' and (resid 225 through 350 )CF225 - 3501 - 106
1717chain 'C' and (resid 351 through 430 )CF351 - 430107 - 186
1818chain 'C' and (resid 431 through 500 )CF431 - 500187 - 256
1919chain 'D' and (resid 16 through 23 )DI16 - 231 - 8
2020chain 'D' and (resid 24 through 33 )DI24 - 339 - 18
2121chain 'D' and (resid 34 through 50 )DI34 - 5019 - 35
2222chain 'D' and (resid 51 through 60 )DI51 - 6036 - 45
2323chain 'D' and (resid 61 through 71 )DI61 - 7146 - 56
2424chain 'D' and (resid 72 through 81 )DI72 - 8157 - 66
2525chain 'D' and (resid 82 through 91 )DI82 - 9167 - 76

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