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Open data
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Basic information
Entry | Database: PDB / ID: 7z74 | ||||||
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Title | PI3KC2a core in complex with PITCOIN2 | ||||||
![]() | Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha | ||||||
![]() | TRANSFERASE / PI3KC2 alpha / kinase | ||||||
Function / homology | ![]() negative regulation of zinc ion transmembrane transport / Synthesis of PIPs at the early endosome membrane / Synthesis of PIPs at the late endosome membrane / Synthesis of PIPs at the Golgi membrane / Synthesis of PIPs at the plasma membrane / autophagosome organization / Golgi Associated Vesicle Biogenesis / phosphatidylinositol-4-phosphate 3-kinase / Clathrin-mediated endocytosis / phosphatidylinositol 3-kinase complex ...negative regulation of zinc ion transmembrane transport / Synthesis of PIPs at the early endosome membrane / Synthesis of PIPs at the late endosome membrane / Synthesis of PIPs at the Golgi membrane / Synthesis of PIPs at the plasma membrane / autophagosome organization / Golgi Associated Vesicle Biogenesis / phosphatidylinositol-4-phosphate 3-kinase / Clathrin-mediated endocytosis / phosphatidylinositol 3-kinase complex / 1-phosphatidylinositol-4-phosphate 3-kinase activity / 1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity / phosphatidylinositol-4,5-bisphosphate 3-kinase / clathrin-coated vesicle / phosphatidylinositol 3-kinase / phosphatidylinositol-3-phosphate biosynthetic process / 1-phosphatidylinositol-3-kinase activity / clathrin binding / exocytosis / phosphatidylinositol-mediated signaling / cellular response to starvation / phosphatidylinositol binding / macroautophagy / trans-Golgi network / endocytosis / cell migration / vesicle / phosphorylation / ATP binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lo, W.T. / Roske, Y. / Daumke, O. / Haucke, V. | ||||||
Funding support | 1items
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![]() | ![]() Title: Development of selective inhibitors of phosphatidylinositol 3-kinase C2 alpha. Authors: Lo, W.T. / Belabed, H. / Kucukdisli, M. / Metag, J. / Roske, Y. / Prokofeva, P. / Ohashi, Y. / Horatscheck, A. / Cirillo, D. / Krauss, M. / Schmied, C. / Neuenschwander, M. / von Kries, J.P. ...Authors: Lo, W.T. / Belabed, H. / Kucukdisli, M. / Metag, J. / Roske, Y. / Prokofeva, P. / Ohashi, Y. / Horatscheck, A. / Cirillo, D. / Krauss, M. / Schmied, C. / Neuenschwander, M. / von Kries, J.P. / Medard, G. / Kuster, B. / Perisic, O. / Williams, R.L. / Daumke, O. / Payrastre, B. / Severin, S. / Nazare, M. / Haucke, V. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 349.8 KB | Display | ![]() |
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PDB format | ![]() | 282.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 791 KB | Display | ![]() |
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Full document | ![]() | 802.4 KB | Display | |
Data in XML | ![]() | 30.8 KB | Display | |
Data in CIF | ![]() | 43 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7z75C ![]() 8a9iC ![]() 7bi4S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 102486.023 Da / Num. of mol.: 1 / Mutation: 533-544/GSGS;550-665/SGAGSGA; F735A;F736A;L809A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q61194, phosphatidylinositol 3-kinase, phosphatidylinositol-4,5-bisphosphate 3-kinase, phosphatidylinositol-4-phosphate 3-kinase | ||||
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#2: Chemical | ChemComp-IKC / ~{ | ||||
#3: Chemical | ChemComp-EDO / #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.5 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion / pH: 7.5 / Details: 0.1MTris, 8-6% PEG3350, 0.2-0.1M MgSO4 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 7, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9184 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→43.26 Å / Num. obs: 41035 / % possible obs: 99.67 % / Redundancy: 5.5 % / CC1/2: 0.998 / Net I/σ(I): 9.02 |
Reflection shell | Resolution: 2.5→2.589 Å / Num. unique obs: 4007 / CC1/2: 0.172 / % possible all: 99.68 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 7BI4 Resolution: 2.5→43.26 Å / SU ML: 0.54 / Cross valid method: THROUGHOUT / σ(F): 1.33 / Phase error: 35.07 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 210.34 Å2 / Biso mean: 86.8764 Å2 / Biso min: 30 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.5→43.26 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 15
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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