根拠: gel filtration, The 14:1 stochiometric complex of GroEL:CnoX was proven by gel filtration, a CnoX-overdosed sample was prepared for single particle cryo-EM, mass spectrometry, in vivo pull ...根拠: gel filtration, The 14:1 stochiometric complex of GroEL:CnoX was proven by gel filtration, a CnoX-overdosed sample was prepared for single particle cryo-EM, mass spectrometry, in vivo pull down of CnoX confirmed GroEL as the only partner, assay for oligomerization, Direct interaction parameters were measured using both AFM and fluorescence anisotropy