+Open data
-Basic information
Entry | Database: PDB / ID: 7y3f | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of the Anabaena PSI-monomer-IsiA supercomplex | ||||||
Components |
| ||||||
Keywords | PHOTOSYNTHESIS / Photosystem I / ELECTRON TRANSPORT / isiA | ||||||
Function / homology | Function and homology information photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosynthetic electron transport chain / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity ...photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosynthetic electron transport chain / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / magnesium ion binding / metal ion binding Similarity search - Function | ||||||
Biological species | Nostoc sp. (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.62 Å | ||||||
Authors | Nagao, R. / Kato, K. / Hamaguchi, T. / Kawakami, K. / Yonekura, K. / Shen, J.R. | ||||||
Funding support | Japan, 1items
| ||||||
Citation | Journal: Nat Commun / Year: 2023 Title: Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120 Authors: Nagao, R. / Kato, K. / Hamaguchi, T. / Ueno, Y. / Tsuboshita, N. / Shimizu, S. / Furutani, M. / Ehira, S. / Nakajima, Y. / Kawakami, K. / Suzuki, T. / Dohmae, N. / Akimoto, S. / Yonekura, K. / Shen, J.R. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7y3f.cif.gz | 966.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7y3f.ent.gz | 848.7 KB | Display | PDB format |
PDBx/mmJSON format | 7y3f.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/y3/7y3f ftp://data.pdbj.org/pub/pdb/validation_reports/y3/7y3f | HTTPS FTP |
---|
-Related structure data
Related structure data | 33593MC M: map data used to model this data C: citing same article (ref.) |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
-Photosystem I ... , 10 types, 10 molecules ABCDEFIJX1
#1: Protein | Mass: 83289.680 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58576, photosystem I |
---|---|
#2: Protein | Mass: 83457.016 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58565, photosystem I |
#3: Protein | Mass: 8825.206 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P0A410, photosystem I |
#4: Protein | Mass: 15176.240 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58573 |
#5: Protein | Mass: 7897.051 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58575 |
#6: Protein | Mass: 17850.568 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58564 |
#7: Protein/peptide | Mass: 5066.925 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58560 |
#8: Protein/peptide | Mass: 5499.422 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58568 |
#11: Protein/peptide | Mass: 4872.792 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: P58566 |
#12: Protein | Mass: 51717.449 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: Q8YQ34 |
-Protein , 3 types, 6 molecules K23645
#9: Protein | Mass: 6996.616 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: Authors do not know the sequence. / Source: (natural) Nostoc sp. (bacteria) | ||
---|---|---|---|
#13: Protein | Mass: 27676.963 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Details: Authors do not know the sequence. / Source: (natural) Nostoc sp. (bacteria) #14: Protein | Mass: 37701.469 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) / References: UniProt: Q8YQ35 |
-Protein/peptide / Sugars , 2 types, 7 molecules M
#10: Protein/peptide | Mass: 4424.250 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Nostoc sp. (bacteria) |
---|---|
#21: Sugar | ChemComp-LMT / |
-Non-polymers , 8 types, 221 molecules
#15: Chemical | ChemComp-CL0 / | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
#16: Chemical | ChemComp-CLA / #17: Chemical | #18: Chemical | #19: Chemical | ChemComp-BCR / #20: Chemical | ChemComp-LHG / #22: Chemical | ChemComp-LMG / | #23: Water | ChemComp-HOH / | |
-Details
Has ligand of interest | N |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: PSI-monomer-IsiA / Type: COMPLEX / Entity ID: #1-#8, #10-#14 / Source: NATURAL | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Molecular weight | Value: 0.64 MDa / Experimental value: NO | |||||||||||||||
Source (natural) | Organism: Nostoc sp. (bacteria) | |||||||||||||||
Buffer solution | pH: 6.5 | |||||||||||||||
Buffer component |
| |||||||||||||||
Specimen | Conc.: 1.68 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES | |||||||||||||||
Specimen support | Grid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R0.6/1 | |||||||||||||||
Vitrification | Instrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277 K |
-Electron microscopy imaging
Microscopy | Model: JEOL CRYO ARM 300 |
---|---|
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1800 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 11.7 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software |
| ||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||
3D reconstruction | Resolution: 2.62 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 47602 / Algorithm: FOURIER SPACE / Symmetry type: POINT | ||||||||||||||||||||||||||||||||||||
Atomic model building | Protocol: FLEXIBLE FIT / Space: RECIPROCAL |