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- EMDB-33593: Structure of the Anabaena PSI-monomer-IsiA supercomplex -

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Basic information

Entry
Database: EMDB / ID: EMD-33593
TitleStructure of the Anabaena PSI-monomer-IsiA supercomplex
Map data
Sample
  • Complex: PSI-monomer-IsiA
    • Protein or peptide: x 13 types
  • Protein or peptide: x 1 types
  • Ligand: x 9 types
Function / homology
Function and homology information


photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosynthetic electron transport chain / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity ...photosystem I reaction center / photosystem I / photosynthetic electron transport in photosystem I / photosynthetic electron transport chain / photosystem I / chlorophyll binding / plasma membrane-derived thylakoid membrane / photosynthesis / 4 iron, 4 sulfur cluster binding / electron transfer activity / magnesium ion binding / metal ion binding
Similarity search - Function
Photosystem I PsaX / Photosystem I PsaX superfamily / PsaX family / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily ...Photosystem I PsaX / Photosystem I PsaX superfamily / PsaX family / Photosystem I PsaL, reaction centre subunit XI / Photosystem I, reaction centre subunit XI / Photosystem I PsaL, reaction centre subunit XI superfamily / Photosystem I reaction centre subunit XI / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII / Photosystem I reaction centre subunit VIII superfamily / Photosystem I PsaF, reaction centre subunit III / Photosystem I PsaF, reaction centre subunit III superfamily / Photosystem I reaction centre subunit III / Photosystem I PsaJ, reaction centre subunit IX / Photosystem I PsaD / Photosystem I PsaJ, reaction centre subunit IX superfamily / Photosystem I, reaction centre subunit PsaD superfamily / Photosystem I reaction centre subunit IX / PsaJ / PsaD / Photosystem antenna protein-like / Photosystem antenna protein-like superfamily / Photosystem II protein / Photosystem I PsaE, reaction centre subunit IV / Photosystem I reaction centre subunit IV / PsaE / Photosystem I protein PsaC / Photosystem I PsaA / Photosystem I PsaB / Photosystem I PsaA/PsaB, conserved site / Photosystem I psaA and psaB proteins signature. / Photosystem I PsaA/PsaB / Photosystem I PsaA/PsaB superfamily / Photosystem I psaA/psaB protein / Electron transport accessory-like domain superfamily / 4Fe-4S dicluster domain / 4Fe-4S ferredoxin, iron-sulphur binding, conserved site / 4Fe-4S ferredoxin-type iron-sulfur binding region signature. / 4Fe-4S ferredoxin-type iron-sulfur binding domain profile. / 4Fe-4S ferredoxin-type, iron-sulphur binding domain
Similarity search - Domain/homology
Photosystem I iron-sulfur center / Photosystem I reaction center subunit VIII / Photosystem I reaction center subunit III / Photosystem I P700 chlorophyll a apoprotein A2 1 / Photosystem I 4.8 kDa protein / Photosystem I reaction center subunit IX / Photosystem I reaction center subunit II / Photosystem I reaction center subunit IV / Photosystem I P700 chlorophyll a apoprotein A1 / Photosystem I reaction center subunit XI / Iron stress-induced chlorophyll-binding protein
Similarity search - Component
Biological speciesNostoc sp. (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.62 Å
AuthorsNagao R / Kato K / Hamaguchi T / Kawakami K / Yonekura K / Shen JR
Funding support Japan, 1 items
OrganizationGrant numberCountry
Japan Society for the Promotion of Science (JSPS)JP20H02914 Japan
CitationJournal: Nat Commun / Year: 2023
Title: Structure of a monomeric photosystem I core associated with iron-stress-induced-A proteins from Anabaena sp. PCC 7120
Authors: Nagao R / Kato K / Hamaguchi T / Ueno Y / Tsuboshita N / Shimizu S / Furutani M / Ehira S / Nakajima Y / Kawakami K / Suzuki T / Dohmae N / Akimoto S / Yonekura K / Shen JR
History
DepositionJun 10, 2022-
Header (metadata) releaseMar 1, 2023-
Map releaseMar 1, 2023-
UpdateMar 1, 2023-
Current statusMar 1, 2023Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_33593.png

Downloads & links

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Map

FileDownload / File: emd_33593.map.gz / Format: CCP4 / Size: 47.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Voxel sizeX=Y=Z: 0.99 Å
Density
Contour LevelBy AUTHOR: 0.016
Minimum - Maximum-0.08383485 - 0.1571778
Average (Standard dev.)6.680899e-05 (±0.0064129266)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions232232232
Spacing232232232
CellA=B=C: 229.68001 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: #1

Fileemd_33593_additional_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_33593_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_33593_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : PSI-monomer-IsiA

EntireName: PSI-monomer-IsiA
Components
  • Complex: PSI-monomer-IsiA
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A1
    • Protein or peptide: Photosystem I P700 chlorophyll a apoprotein A2 1
    • Protein or peptide: Photosystem I iron-sulfur center
    • Protein or peptide: Photosystem I reaction center subunit II
    • Protein or peptide: Photosystem I reaction center subunit IV
    • Protein or peptide: Photosystem I reaction center subunit III
    • Protein or peptide: Photosystem I reaction center subunit VIII
    • Protein or peptide: Photosystem I reaction center subunit IX
    • Protein or peptide: PsaM
    • Protein or peptide: Photosystem I 4.8 kDa protein
    • Protein or peptide: Photosystem I reaction center subunit XI
    • Protein or peptide: IsiA
    • Protein or peptide: Iron stress-induced chlorophyll-binding protein
  • Protein or peptide: Unknown
  • Ligand: CHLOROPHYLL A ISOMER
  • Ligand: CHLOROPHYLL A
  • Ligand: PHYLLOQUINONEPhytomenadione
  • Ligand: IRON/SULFUR CLUSTERIron–sulfur cluster
  • Ligand: BETA-CAROTENEΒ-Carotene
  • Ligand: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE
  • Ligand: DODECYL-BETA-D-MALTOSIDE
  • Ligand: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE
  • Ligand: water

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Supramolecule #1: PSI-monomer-IsiA

SupramoleculeName: PSI-monomer-IsiA / type: complex / ID: 1 / Chimera: Yes / Parent: 0 / Macromolecule list: #1-#8, #10-#14
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 640 KDa

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Macromolecule #1: Photosystem I P700 chlorophyll a apoprotein A1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A1 / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 83.28968 KDa
SequenceString: MTISPPEREE KKARVIVDKD PVPTSFEKWA QPGHFDRTLA RGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGH LAVVTIWLS GMIFHGAKFS NYEAWLSDPL NVRPSAQVVW PIVGQDILNG DVGGGFHGIQ ITSGLFQVWR GWGITNSFQL Y CTAIGGLV ...String:
MTISPPEREE KKARVIVDKD PVPTSFEKWA QPGHFDRTLA RGPKTTTWIW NLHALAHDFD THTSDLEDIS RKIFAAHFGH LAVVTIWLS GMIFHGAKFS NYEAWLSDPL NVRPSAQVVW PIVGQDILNG DVGGGFHGIQ ITSGLFQVWR GWGITNSFQL Y CTAIGGLV LAGLFLFAGW FHYHKRAPKL EWFQNVESML NHHLQVLLGC GSLGWAGHLI HVSAPINKLM DAGVAVKDIP LP HEFILNK SLLIDLFPGF AAGLTPFFTL NWGQYADFLT FKGGLNPVTG GLWMTDIAHH HLAIAVVFII AGHQYRTNWG IGH SIKEIL ENHKGPFTGE GHKGLYENLT TSWHAQLATN LAFLGSLTII IAHHMYAMPP YPYLATDYAT QLCIFTHHIW IGGF LIVGG AAHAAIFMVR DYDPVVNQNN VLDRVIRHRD AIISHLNWVC IFLGFHSFGL YIHNDTMRAL GRPQDMFSDT AIQLQ PVFA QWVQNLHTLA PGGTAPNALE PVSYAFGGGV LAVGGKVAMM PIALGTADFL IHHIHAFTIH VTVLILLKGV LFARSS RLI PDKANLGFRF PCDGPGRGGT CQVSGWDHVF LGLFWMYNSL SIVIFHFSWK MQSDVWGTVD AAGNVSHITG GNFAQSA IT INGWLRDFLW AQASQVINSY GSALSAYGLM FLGAHFVWAF SLMFLFSGRG YWQELIESIV WAHNKLKVAP AIQPRALS I TQGRAVGVAH YLLGGIATTW AFFHAHILSV G

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Macromolecule #2: Photosystem I P700 chlorophyll a apoprotein A2 1

MacromoleculeName: Photosystem I P700 chlorophyll a apoprotein A2 1 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 83.457016 KDa
SequenceString: MATKFPKFSQ DLAQDPTTRR IWYAMAMGND FESHDGMTEE NLYQKIFATH FGHLAIIFLW ASSLLFHVAW QGNFEQWIKD PLHVRPIAH AIWDPHFGKP AIEAFTQAGA NGPVNIAYSG VYHWWYTIGM RTNTELYTGS VFLLLFASLF LFAGWLHLQP K FRPSLAWF ...String:
MATKFPKFSQ DLAQDPTTRR IWYAMAMGND FESHDGMTEE NLYQKIFATH FGHLAIIFLW ASSLLFHVAW QGNFEQWIKD PLHVRPIAH AIWDPHFGKP AIEAFTQAGA NGPVNIAYSG VYHWWYTIGM RTNTELYTGS VFLLLFASLF LFAGWLHLQP K FRPSLAWF KSAESRLNHH LAGLFGVSSL AWAGHLIHVA IPESRGQHVG WDNFLSTAPH PAGLQPFFTG NWGVYAQNPD TA GHIFSTS QGAGTAILTF LGGFHPQTES LWLTDMAHHH LAIAVLFIVA GHMYRTNFGI GHSIKEMMNA KTFFGKPVEG PFN MPHQGI YDTYNNSLHF QLGWHLACLG VVTSWVAQHM YSLPSYAFIA KDYTTQAALY THHQYIAIFL MVGAFAHGAI FLVR DYDPE QNKGNVLERV LQHKEAIISH LSWVSLFLGF HTLGLYVHND VVVAFGTPEK QILIEPVFAQ FIQAAHGKVL YGLDT LLSN PDSVAYTAYP NYANVWLPGW LDAINSGTNS LFLTIGPGDF LVHHAIALGL HTTTLILVKG ALDARGSKLM PDKKDF GYA FPCDGPGRGG TCDISAWDSF YLSLFWALNT VGWVTFYWHW KHLGIWQGNV AQFNENSTYL MGWFRDYLWA NSAQLIN GY NPYGVNNLSV WAWMFLFGHL VWATGFMFLI SWRGYWQELI ETLVWAHERT PIANLVRWKD KPVALSIVQA RVVGLAHF T VGYVLTYAAF LIASTAGKFG

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Macromolecule #3: Photosystem I iron-sulfur center

MacromoleculeName: Photosystem I iron-sulfur center / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO / EC number: photosystem I
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 8.825206 KDa
SequenceString:
MSHTVKIYDT CIGCTQCVRA CPTDVLEMVP WDGCKAAQVA SSPRTEDCVG CKRCETACPT DFLSIRVYLG AETTRSMGLA Y

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Macromolecule #4: Photosystem I reaction center subunit II

MacromoleculeName: Photosystem I reaction center subunit II / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 15.17624 KDa
SequenceString:
MAETLSGKTP LFAGSTGGLL TKAVEEEKYA ITWTSPKAQV FELPTGGAAT MHEGENLLYI ARKEYGIALG GQLRKFKITN YKIYRILPS GETTFIHPAD GVFPEKVNAG REKVRFNARS IGENPNPSQV KFSGKATYDA

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Macromolecule #5: Photosystem I reaction center subunit IV

MacromoleculeName: Photosystem I reaction center subunit IV / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 7.897051 KDa
SequenceString:
MVQRGSKVRI LRPESYWFQD VGTVASVDQS GIKYPVIVRF DKVNYAGINT NNFAVDELIE VEAPKAKAKK

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Macromolecule #6: Photosystem I reaction center subunit III

MacromoleculeName: Photosystem I reaction center subunit III / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 17.850568 KDa
SequenceString:
MRRLFALILV ICLSFSFAPP AKALGADLTP CAENPAFQAL AKNARNTTAD PQSGQKRFER YSQALCGPEG YPHLIVDGRL DRAGDFLIP SILFLYIAGW IGWVGRAYLQ AIKKDSDTEQ KEIQLDLGIA LPIIATGFAW PAAAVKELLS GELTAKDSEI T VSPR

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Macromolecule #7: Photosystem I reaction center subunit VIII

MacromoleculeName: Photosystem I reaction center subunit VIII / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 5.066925 KDa
SequenceString:
MATAFLPSIL ADASFLSSIF VPVIGWVVPI ATFSFLFLYI EREDVA

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Macromolecule #8: Photosystem I reaction center subunit IX

MacromoleculeName: Photosystem I reaction center subunit IX / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 5.499422 KDa
SequenceString:
MADKADQSSY LIKFISTAPV AATIWLTITA GILIEFNRFF PDLLFHPLP

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Macromolecule #9: Unknown

MacromoleculeName: Unknown / type: protein_or_peptide / ID: 9 / Details: Authors do not know the sequence. / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 6.996616 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)

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Macromolecule #10: PsaM

MacromoleculeName: PsaM / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 4.42425 KDa
SequenceString:
MPTLYLAQVS SISDTQVYIA LVVALIPGLL AWRLATELYK

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Macromolecule #11: Photosystem I 4.8 kDa protein

MacromoleculeName: Photosystem I 4.8 kDa protein / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 4.872792 KDa
SequenceString:
MAKAKISPVA NTGAKPPYTF RTGWALLLLA VNFLVAAYYF HIIQ

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Macromolecule #12: Photosystem I reaction center subunit XI

MacromoleculeName: Photosystem I reaction center subunit XI / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 51.717449 KDa
SequenceString: MTTATINPQQ YGWWAGNARF INLSGRLLGA HIAHAGLIIL WAGAMTLFEI TKYNPSLPIY EQGLILLPHL ATLGFGIGDG GQIIDTYPY FVIGVVHLVS SAVLAAGGIY HALLGPEVLP ENNQFPGFFG YDWEDEDKMT TIIGIHLLLL GAGAWLLVAK A LFWGGLYD ...String:
MTTATINPQQ YGWWAGNARF INLSGRLLGA HIAHAGLIIL WAGAMTLFEI TKYNPSLPIY EQGLILLPHL ATLGFGIGDG GQIIDTYPY FVIGVVHLVS SAVLAAGGIY HALLGPEVLP ENNQFPGFFG YDWEDEDKMT TIIGIHLLLL GAGAWLLVAK A LFWGGLYD STVASVRVIT EPTVNPARIF GYLFGAFGKQ GMAAVNNLED VVGGHIWVGI LCIGGGFWHI LTQPFAWAKK VL FWSGEAY LSYSLAALAY MGLLAAYFVT VNDTVYPTEF YGPLGFSSTS GVISVRTWLA TSHFALAIVF LSGHIWHALR VRV LEAGLN FEQGVVNYLD TPELGNLQTP INTSDLTLKF LVNLPIYRPG LSAFARGLEI GMAHGYFLLG PFVKLGPLRN TEFA NQAGL LATIGLLLIL SICLWLYGSA WFQEGKSPQG ELPENLKTAK SWSEFNAGWI VGSCGGALFA YLLVTNSSLF F

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Macromolecule #13: IsiA

MacromoleculeName: IsiA / type: protein_or_peptide / ID: 13 / Details: Authors do not know the sequence. / Number of copies: 3 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 27.676963 KDa
SequenceString: (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) ...String:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #14: Iron stress-induced chlorophyll-binding protein

MacromoleculeName: Iron stress-induced chlorophyll-binding protein / type: protein_or_peptide / ID: 14 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Nostoc sp. (bacteria)
Molecular weightTheoretical: 37.701469 KDa
SequenceString: MQTYDNPNIK YDWWAGNARF ANLSGLFIGA HVAQAALTTL WAGAFTWFEI SRYKPEIPMG EQGLILLPHL ATLGFGVGVS GQVVNTYPY FVIGALHLIS SAVLGAGALF HTFKGPRNLK NTTGSARKFH FEWNDPKQLG LILGHHLLFL GMAALLLVGK A MFWGGLYD ...String:
MQTYDNPNIK YDWWAGNARF ANLSGLFIGA HVAQAALTTL WAGAFTWFEI SRYKPEIPMG EQGLILLPHL ATLGFGVGVS GQVVNTYPY FVIGALHLIS SAVLGAGALF HTFKGPRNLK NTTGSARKFH FEWNDPKQLG LILGHHLLFL GMAALLLVGK A MFWGGLYD ATTQVVRVVN HPTLNPFVIY GYQTHFASVN NLEDLVGGHI YVGLILIGGG IWHIVKEPLP WAKKLLIFSG EA ILSYSLG GIALAGFVAA YFCAVNTLAY PVEFYGAPLE LKFGVTPYFA DTVKLADGGY SARAWLANAH FFLAFFFLQG HLW HALRAI GVDFRQIEKS LNAISSAE

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Macromolecule #15: CHLOROPHYLL A ISOMER

MacromoleculeName: CHLOROPHYLL A ISOMER / type: ligand / ID: 15 / Number of copies: 1 / Formula: CL0
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CL0:
CHLOROPHYLL A ISOMER / Chlorophyll a

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Macromolecule #16: CHLOROPHYLL A

MacromoleculeName: CHLOROPHYLL A / type: ligand / ID: 16 / Number of copies: 155 / Formula: CLA
Molecular weightTheoretical: 893.489 Da
Chemical component information

ChemComp-CLA:
CHLOROPHYLL A / Chlorophyll a

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Macromolecule #17: PHYLLOQUINONE

MacromoleculeName: PHYLLOQUINONE / type: ligand / ID: 17 / Number of copies: 2 / Formula: PQN
Molecular weightTheoretical: 450.696 Da
Chemical component information

ChemComp-PQN:
PHYLLOQUINONE / Phytomenadione

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Macromolecule #18: IRON/SULFUR CLUSTER

MacromoleculeName: IRON/SULFUR CLUSTER / type: ligand / ID: 18 / Number of copies: 3 / Formula: SF4
Molecular weightTheoretical: 351.64 Da
Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

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Macromolecule #19: BETA-CAROTENE

MacromoleculeName: BETA-CAROTENE / type: ligand / ID: 19 / Number of copies: 29 / Formula: BCR
Molecular weightTheoretical: 536.873 Da
Chemical component information

ChemComp-BCR:
BETA-CAROTENE / Β-Carotene

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Macromolecule #20: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE

MacromoleculeName: 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / type: ligand / ID: 20 / Number of copies: 4 / Formula: LHG
Molecular weightTheoretical: 722.97 Da
Chemical component information

ChemComp-LHG:
1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE / phospholipid*YM / Phosphatidylglycerol

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Macromolecule #21: DODECYL-BETA-D-MALTOSIDE

MacromoleculeName: DODECYL-BETA-D-MALTOSIDE / type: ligand / ID: 21 / Number of copies: 6 / Formula: LMT
Molecular weightTheoretical: 510.615 Da
Chemical component information

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

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Macromolecule #22: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

MacromoleculeName: 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE / type: ligand / ID: 22 / Number of copies: 1 / Formula: LMG
Molecular weightTheoretical: 787.158 Da
Chemical component information

ChemComp-LMG:
1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE

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Macromolecule #23: water

MacromoleculeName: water / type: ligand / ID: 23 / Number of copies: 26 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER / Water

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration1.68 mg/mL
BufferpH: 6.5
Component:
ConcentrationFormulaName
20.0 mMMES-NaOHMES
0.03 %DDMDDM
GridModel: Quantifoil R0.6/1 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeJEOL CRYO ARM 300
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 1.8 µm / Nominal defocus min: 0.8 µm
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Detector mode: COUNTING / Average electron dose: 11.7 e/Å2

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Image processing

Startup modelType of model: INSILICO MODEL
In silico model: An initial model was generated de novo from 2D classification.
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final 3D classificationSoftware - Name: RELION (ver. 3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.1)
Final reconstructionAlgorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 2.62 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 3.1) / Number images used: 47602
FSC plot (resolution estimation)

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Atomic model buiding 1

RefinementSpace: RECIPROCAL / Protocol: FLEXIBLE FIT
Output model

PDB-7y3f:
Structure of the Anabaena PSI-monomer-IsiA supercomplex

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