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- PDB-7xy8: Crystal structure of antibody Fab fragment in complex with CD147(... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7xy8 | ||||||
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Title | Crystal structure of antibody Fab fragment in complex with CD147(EMMPIRIN) | ||||||
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![]() | PROTEIN BINDING / cd147 / complex / antibody | ||||||
Function / homology | ![]() Defective SLC16A1 causes symptomatic deficiency in lactate transport (SDLT) / Proton-coupled monocarboxylate transport / positive regulation of matrix metallopeptidase secretion / acrosomal membrane / dendrite self-avoidance / response to mercury ion / cell-cell adhesion mediator activity / neural retina development / endothelial tube morphogenesis / Pyruvate metabolism ...Defective SLC16A1 causes symptomatic deficiency in lactate transport (SDLT) / Proton-coupled monocarboxylate transport / positive regulation of matrix metallopeptidase secretion / acrosomal membrane / dendrite self-avoidance / response to mercury ion / cell-cell adhesion mediator activity / neural retina development / endothelial tube morphogenesis / Pyruvate metabolism / photoreceptor cell maintenance / Basigin interactions / Aspirin ADME / homophilic cell adhesion via plasma membrane adhesion molecules / odontogenesis of dentin-containing tooth / D-mannose binding / decidualization / photoreceptor outer segment / positive regulation of vascular endothelial growth factor production / Integrin cell surface interactions / response to cAMP / embryo implantation / Degradation of the extracellular matrix / photoreceptor inner segment / positive regulation of endothelial cell migration / neutrophil chemotaxis / protein localization to plasma membrane / axon guidance / sarcolemma / response to peptide hormone / positive regulation of interleukin-6 production / melanosome / virus receptor activity / signaling receptor activity / basolateral plasma membrane / angiogenesis / positive regulation of viral entry into host cell / cell surface receptor signaling pathway / endosome / cadherin binding / axon / Golgi membrane / intracellular membrane-bounded organelle / focal adhesion / endoplasmic reticulum membrane / mitochondrion / extracellular exosome / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() synthetic construct (others) | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nakamura, K. / Amano, M. / Yoneda, K. / Suzuki, M. / Fukuchi, K. | ||||||
Funding support | 1items
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![]() | ![]() Title: Novel Antibody Exerts Antitumor Effect through Downregulation of CD147 and Activation of Multiple Stress Signals. Authors: Fukuchi, K. / Nanai, K. / Yuita, H. / Maru, C. / Tsukada, J. / Ishigami, M. / Nagai, Y. / Nakano, Y. / Yoshimura, C. / Yoneda, K. / Amano, M. / Nakamura, K. / Oda, Y. / Nishigohri, H. / ...Authors: Fukuchi, K. / Nanai, K. / Yuita, H. / Maru, C. / Tsukada, J. / Ishigami, M. / Nagai, Y. / Nakano, Y. / Yoshimura, C. / Yoneda, K. / Amano, M. / Nakamura, K. / Oda, Y. / Nishigohri, H. / Yamamoto, S. / Ohnishi-Totoki, Y. / Inaki, K. / Komori, H. / Nakano, R. / Kanari, Y. / Nishida, A. / Matsui, Y. / Funo, S. / Takahashi, S. / Ohtsuka, T. / Agatsuma, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 231.8 KB | Display | ![]() |
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PDB format | ![]() | 183.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 477.9 KB | Display | ![]() |
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Full document | ![]() | 493.5 KB | Display | |
Data in XML | ![]() | 40.7 KB | Display | |
Data in CIF | ![]() | 57.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3b5hS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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Components
#1: Protein | Mass: 22503.928 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Antibody | Mass: 25277.320 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() #3: Antibody | Mass: 23432.900 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) / Production host: ![]() #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.09 Å3/Da / Density % sol: 41.23 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7 Details: 0.1M Sodium Malonate pH 7.0, 12% (w/v) Polyethylene Glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Ambient temp details: Nitrogen gas flow (95K-300K) / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 25, 2018 |
Radiation | Monochromator: Si(111) double crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→35.911 Å / Num. obs: 51301 / % possible obs: 98.4 % / Redundancy: 3 % / Rpim(I) all: 0.06 / Rrim(I) all: 0.105 / Rsym value: 0.086 / Net I/av σ(I): 5.4 / Net I/σ(I): 7.8 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 3 % / Rmerge(I) obs: 0.36 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 7430 / Rpim(I) all: 0.247 / Rrim(I) all: 0.439 / Rsym value: 0.36 / % possible all: 97.8 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3b5h Resolution: 2.3→35.91 Å / Cor.coef. Fo:Fc: 0.92 / Cor.coef. Fo:Fc free: 0.879 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.455 / ESU R Free: 0.282 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 143.51 Å2 / Biso mean: 43.373 Å2 / Biso min: 15.57 Å2
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Refinement step | Cycle: final / Resolution: 2.3→35.91 Å
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Refine LS restraints |
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Refine LS restraints NCS | Refine-ID: X-RAY DIFFRACTION / Type: interatomic distance / Weight position: 0.05
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LS refinement shell | Resolution: 2.3→2.359 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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