- PDB-7xm0: Crystal structure of Sau3AI-C and DNA substrate complex -
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Basic information
Entry
Database: PDB / ID: 7xm0
Title
Crystal structure of Sau3AI-C and DNA substrate complex
Components
DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3')
Type II restriction enzyme Sau3AI
Keywords
DNA BINDING PROTEIN/DNA / Sau3AI / restriction endonuclease / Protein-DNA complex / enzyme mechanism / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information
type II site-specific deoxyribonuclease / type II site-specific deoxyribonuclease activity / DNA restriction-modification system / DNA binding Similarity search - Function
DNA mismatch repair MutH/Type II restriction enzyme Sau3AI / DNA mismatch repair enzyme MutH / DNA mismatch repair enzyme MutH / DNA mismatch repair MutH/Type II restriction endonuclease superfamily / Restriction endonuclease type II-like Similarity search - Domain/homology
A: Type II restriction enzyme Sau3AI B: Type II restriction enzyme Sau3AI C: Type II restriction enzyme Sau3AI D: DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3') E: DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3') F: DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3') G: DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3') H: DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3') I: DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3') hetero molecules
Mass: 33401.582 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Gene: sau3AIR / Production host: Escherichia coli (E. coli) References: UniProt: P16667, type II site-specific deoxyribonuclease
#2: DNA chain
DNA (5'-D(*CP*AP*TP*GP*AP*TP*CP*AP*TP*G)-3')
Mass: 3044.017 Da / Num. of mol.: 6 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)