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Open data
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Basic information
Entry | Database: PDB / ID: 7xg8 | ||||||
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Title | Crystal structure of PstS protein from cyanophage P-SSM2 | ||||||
![]() | ABC transporter, substrate binding protein, phosphate![]() | ||||||
![]() | ![]() ![]() ![]() | ||||||
Function / homology | Phosphate ABC transporter, substrate-binding protein PstS / PBP superfamily domain / PBP domain / phosphate ion transmembrane transport / ![]() ![]() ![]() ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cai, K. / Jiang, Y.L. | ||||||
Funding support | 1items
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![]() | ![]() Title: Biochemical and structural characterization of the cyanophage-encoded phosphate-binding protein: implications for enhanced phosphate uptake of infected cyanobacteria Authors: Zhao, F. / Lin, X. / Cai, K. / Jiang, Y. / Ni, T. / Chen, Y. / Feng, J. / Dang, S. / Zhou, C.Z. / Zeng, Q. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.9 KB | Display | ![]() |
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PDB format | ![]() | 49.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7xg7C ![]() 2abhS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | ![]() Mass: 33819.359 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-PO4 / ![]() |
#3: Water | ChemComp-HOH / ![]() |
Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.5 % |
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Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop Details: 25% (w/v) polyethylene glycol 3350, 0.1 M citric acid, pH 3.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 27, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.25→50 Å / Num. obs: 13262 / % possible obs: 99.7 % / Redundancy: 7.2 % / Rmerge(I) obs: 0.13 / Rsym value: 0.13 / Net I/σ(I): 17.965 |
Reflection shell | Resolution: 2.25→2.33 Å / Redundancy: 7.2 % / Rmerge(I) obs: 0.37 / Mean I/σ(I) obs: 8.132 / Num. unique obs: 1287 / Rsym value: 0.37 / % possible all: 99.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 2abh Resolution: 2.25→50 Å / Cor.coef. Fo:Fc: 0.9 / Cor.coef. Fo:Fc free: 0.859 / SU B: 7.451 / SU ML: 0.187 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.41 / ESU R Free: 0.27 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 52.4 Å2 / Biso mean: 20.523 Å2 / Biso min: 7.36 Å2
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Refinement step | Cycle: final / Resolution: 2.25→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.25→2.308 Å / Rfactor Rfree error: 0
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