+Open data
-Basic information
Entry | Database: PDB / ID: 7x6g | ||||||
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Title | Outer membrane lipoprotein QseG of Escherichia coli O157:H7 | ||||||
Components | Quorum-sensing regulator protein G | ||||||
Keywords | SIGNALING PROTEIN / Enterohemorrhagic Escherichia coli / Two-component systems / Outer membrane lipoprotein | ||||||
Function / homology | Quorum-sensing regulator protein G / YfhG lipoprotein / cell outer membrane / Prokaryotic membrane lipoprotein lipid attachment site profile. / Quorum-sensing regulator protein G Function and homology information | ||||||
Biological species | Escherichia coli O157:H7 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | ||||||
Authors | Matsumoto, K. / Fukuda, Y. / Inoue, T. | ||||||
Funding support | Japan, 1items
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Citation | Journal: Acta Crystallogr.,Sect.F / Year: 2023 Title: Crystal structures of QseE and QseG: elements of a three-component system from Escherichia coli. Authors: Matsumoto, K. / Fukuda, Y. / Inoue, T. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7x6g.cif.gz | 198.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7x6g.ent.gz | 153.2 KB | Display | PDB format |
PDBx/mmJSON format | 7x6g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x6/7x6g ftp://data.pdbj.org/pub/pdb/validation_reports/x6/7x6g | HTTPS FTP |
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-Related structure data
Related structure data | 7x6fC 7x6hC 8jwdC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 20422.787 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli O157:H7 (bacteria) / Gene: qseG, yfhG, Z3831, ECs3421 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0AD45 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.16 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.32 M Lithium chloride, 0.1 M Sodium citrate pH 5.5, 14 % w/v PEG 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL44XU / Wavelength: 0.9 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 1, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→43.86 Å / Num. obs: 45799 / % possible obs: 98.3 % / Redundancy: 3.4 % / CC1/2: 0.997 / Rmerge(I) obs: 0.07 / Rpim(I) all: 0.045 / Rrim(I) all: 0.083 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 2.35→2.43 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.851 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 4566 / CC1/2: 0.613 / Rpim(I) all: 0.549 / Rrim(I) all: 1.015 / % possible all: 99.9 |
-Processing
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: AlphaFold structure Resolution: 2.35→37.854 Å / Cor.coef. Fo:Fc: 0.941 / Cor.coef. Fo:Fc free: 0.908 / SU B: 11.891 / SU ML: 0.273 / Cross valid method: FREE R-VALUE / ESU R: 0.436 / ESU R Free: 0.295 Details: Hydrogens have been used if present in the input file
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 63.532 Å2
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Refinement step | Cycle: LAST / Resolution: 2.35→37.854 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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