+
Open data
-
Basic information
Entry | Database: PDB / ID: 7x0f | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of Pseudomonas NRPS protein, AmbB-TC bound to Ppant | ||||||
![]() | AMB antimetabolite synthase AmbB | ||||||
![]() | BIOSYNTHETIC PROTEIN / Non-ribosomal peptide synthetase / AmbB / Pseudomonas | ||||||
Function / homology | ![]() L-alanine-[L-alanyl-carrier protein] ligase / amino acid activation for nonribosomal peptide biosynthetic process / secondary metabolite biosynthetic process / ligase activity / phosphopantetheine binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | ChuYuanKee, M. / Bharath, S.R. / Song, H. | ||||||
Funding support | 1items
| ||||||
![]() | ![]() Title: Structural insights into the substrate-bound condensation domains of non-ribosomal peptide synthetase AmbB. Authors: Chu Yuan Kee, M.J. / Bharath, S.R. / Wee, S. / Bowler, M.W. / Gunaratne, J. / Pan, S. / Zhang, L. / Song, H. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 362.8 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 292.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.2 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1.2 MB | Display | |
Data in XML | ![]() | 68.7 KB | Display | |
Data in CIF | ![]() | 96.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7x0eSC ![]() 7x17C S: Starting model for refinement C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
3 | ![]()
| ||||||||
4 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 55970.652 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() References: UniProt: Q9I1H0, L-alanine-[L-alanyl-carrier protein] ligase #2: Chemical | ChemComp-PNS / #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.56 Å3/Da / Density % sol: 51.99 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion Details: 0.1M MES pH 6.6, 0.2M ammonium nitrate, 15% PEG 3350, 5% glycerol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Aug 31, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→49.56 Å / Num. obs: 114170 / % possible obs: 99 % / Redundancy: 3.4 % / CC1/2: 0.997 / Net I/σ(I): 11.4 |
Reflection shell | Resolution: 2.2→2.26 Å / Redundancy: 2.6 % / Mean I/σ(I) obs: 2.1 / Num. unique obs: 5650 / CC1/2: 0.507 / % possible all: 99 |
-
Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]() Starting model: 7X0E Resolution: 2.2→49.56 Å / Cross valid method: THROUGHOUT
| ||||||||||||||||||||
Displacement parameters | Biso max: 163.46 Å2 / Biso mean: 40.8412 Å2 / Biso min: 13.57 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→49.56 Å
| ||||||||||||||||||||
LS refinement shell | Resolution: 2.2→2.26 Å
|