- PDB-7w7r: High resolution structure of a fish aquaporin reveals a novel ext... -
+
データを開く
IDまたはキーワード:
読み込み中...
-
基本情報
登録情報
データベース: PDB / ID: 7w7r
タイトル
High resolution structure of a fish aquaporin reveals a novel extracellular fold.
要素
Aquaporin 1
キーワード
MEMBRANE PROTEIN / Aquaporin / fish / Anabas testudineus / Pichia pastoris / transport protein
機能・相同性
Aquaporin transporter / Major intrinsic protein, conserved site / MIP family signature. / Major intrinsic protein / Major intrinsic protein / Aquaporin-like / channel activity / membrane => GO:0016020 / Aquaporin 1
温度: 277.15 K / 手法: 蒸気拡散法, ハンギングドロップ法 詳細: reservoir solution: 0.3M Lithium sulfate, 0.1M ADA (pH 6.5), 30% v/v PEG 400. Prior to setting up the crystallization drops, 4 micro liter of the reservoir solution was mixed with 1 micro ...詳細: reservoir solution: 0.3M Lithium sulfate, 0.1M ADA (pH 6.5), 30% v/v PEG 400. Prior to setting up the crystallization drops, 4 micro liter of the reservoir solution was mixed with 1 micro liter 30% w/v Dextran sulfate sodium salt Mr 5000. Crystallization drops were set up by mixing the reservoir/additive mixture with protein at 1:1 or 2:1 ratio and the drops were left to equilibrate against 0.5 milliliter reservoir at room temperature and crystal grew in cold room (4 degree)