+Open data
-Basic information
Entry | Database: PDB / ID: 7w62 | ||||||
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Title | Mevo lectin complex with mannotetraose (Man4) | ||||||
Components | lectin | ||||||
Keywords | SUGAR BINDING PROTEIN / Mevo lectin / mannose-binding lectin / heptamer | ||||||
Function / homology | Jacalin-like lectin domain superfamily / Jacalin-type lectin domain-containing protein Function and homology information | ||||||
Biological species | Methanococcus voltae A3 (archaea) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Sivaji, N. / Vijayan, M. | ||||||
Funding support | India, 1items
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Citation | Journal: To Be Published Title: Mevo lectin complex with mannotetraose (Man4) Authors: Sivaji, N. / Vijayan, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7w62.cif.gz | 397.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7w62.ent.gz | 326.2 KB | Display | PDB format |
PDBx/mmJSON format | 7w62.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w6/7w62 ftp://data.pdbj.org/pub/pdb/validation_reports/w6/7w62 | HTTPS FTP |
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-Related structure data
Related structure data | 7dedS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 15783.812 Da / Num. of mol.: 14 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Methanococcus voltae A3 (archaea) / Strain: ATCC BAA-1334 / A3 / Gene: Mvol_0737 / Production host: Escherichia coli (E. coli) / References: UniProt: D7DTD6 #2: Polysaccharide | alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose Type: oligosaccharide / Mass: 666.578 Da / Num. of mol.: 12 / Source method: obtained synthetically #3: Polysaccharide | #4: Chemical | ChemComp-EDO / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.9 Å3/Da / Density % sol: 57.75 % |
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Crystal grow | Temperature: 295 K / Method: microbatch / pH: 6 Details: Tacsimate pH 6.0, 0.1 M MES monohydrate pH 6.0, 25% (w/v) polyethylene glycol 4000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ELETTRA / Beamline: 11.2C / Wavelength: 1 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Aug 5, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2→96.21 Å / Num. obs: 2231847 / % possible obs: 100 % / Redundancy: 12 % / CC1/2: 0.99 / Rmerge(I) obs: 0.14 / Net I/σ(I): 12.1 |
Reflection shell | Resolution: 2→2.11 Å / Rmerge(I) obs: 1.33 / Num. unique obs: 186472 / CC1/2: 0.68 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7DED Resolution: 2→85.27 Å / Cor.coef. Fo:Fc: 0.949 / Cor.coef. Fo:Fc free: 0.937 / Cross valid method: THROUGHOUT / ESU R: 0.174 / ESU R Free: 0.153 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.575 Å2
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Refinement step | Cycle: LAST / Resolution: 2→85.27 Å
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Refine LS restraints |
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