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- PDB-7w4t: Structure of the M. tuberculosis HtrA S367A mutant at room-temperature -
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Open data
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Basic information
Entry | Database: PDB / ID: 7w4t | ||||||
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Title | Structure of the M. tuberculosis HtrA S367A mutant at room-temperature | ||||||
![]() | Probable serine protease HtrA1 | ||||||
![]() | HYDROLASE / HtrA family of serine protease / chymotrypsin-like / Periplasm / Protein quality control / signal transduction and room-temperature data | ||||||
Function / homology | ![]() peptidase Do / peptidoglycan-based cell wall / serine-type endopeptidase activity / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gupta, A.K. / Gopal, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Allosteric Determinants in High Temperature Requirement A Enzymes Are Conserved and Regulate the Population of Active Conformations. Authors: Gupta, A.K. / Singh, K. / Patidar, Y. / Sharma, R. / Sardesai, A.A. / Reddy, G. / Gopal, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.7 KB | Display | ![]() |
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PDB format | ![]() | 48 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 420.8 KB | Display | ![]() |
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Full document | ![]() | 422.5 KB | Display | |
Data in XML | ![]() | 12.2 KB | Display | |
Data in CIF | ![]() | 16.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7vyzC ![]() 7vz0C ![]() 7w21C ![]() 7w22C ![]() 7w23C ![]() 7w24C ![]() 7w25C ![]() 7w4rC ![]() 7w4sC ![]() 7w4uC ![]() 7w4vC ![]() 7w4wC ![]() 6ieoS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 32506.391 Da / Num. of mol.: 1 / Mutation: S367A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 25618 / H37Rv / Gene: htrA1, degP, htrA, Rv1223 / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.81 % |
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Crystal grow | Temperature: 298 K / Method: microbatch Details: 0.1M HEPES, 1.4M Sodium Citrate tribasic dihydrate pH 7.5 |
-Data collection
Diffraction | Mean temperature: 293 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Nov 20, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→37.69 Å / Num. obs: 14067 / % possible obs: 99.95 % / Redundancy: 2.5 % / CC1/2: 0.991 / Net I/σ(I): 7 |
Reflection shell | Resolution: 2.2→2.279 Å / Num. unique obs: 1407 / CC1/2: 0.469 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6IEO Resolution: 2.2→37.69 Å / SU ML: 0.29 / Cross valid method: FREE R-VALUE / σ(F): 1.99 / Phase error: 26.66 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.2→37.69 Å
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Refine LS restraints |
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LS refinement shell |
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