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Open data
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Basic information
Entry | Database: PDB / ID: 7w25 | ||||||
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Title | Structure of the M. tuberculosis HtrA S413A mutant | ||||||
![]() | Probable serine protease HtrA1 | ||||||
![]() | HYDROLASE / HtrA family of serine protease / chymotrypsin-like / Periplasm / Protein quality control and signal transduction. | ||||||
Function / homology | ![]() peptidase Do / peptidoglycan-based cell wall / serine-type endopeptidase activity / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Gupta, A.K. / Gopal, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Allosteric Determinants in High Temperature Requirement A Enzymes Are Conserved and Regulate the Population of Active Conformations. Authors: Gupta, A.K. / Singh, K. / Patidar, Y. / Sharma, R. / Sardesai, A.A. / Reddy, G. / Gopal, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 68.5 KB | Display | ![]() |
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PDB format | ![]() | 47.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 418.3 KB | Display | ![]() |
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Full document | ![]() | 419.8 KB | Display | |
Data in XML | ![]() | 12.5 KB | Display | |
Data in CIF | ![]() | 16.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7vyzC ![]() 7vz0C ![]() 7w21C ![]() 7w22C ![]() 7w23C ![]() 7w24C ![]() 7w4rC ![]() 7w4sC ![]() 7w4tC ![]() 7w4uC ![]() 7w4vC ![]() 7w4wC ![]() 6ieoS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 31458.238 Da / Num. of mol.: 1 / Mutation: S413A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 25618 / H37Rv / Gene: htrA1, degP, htrA, Rv1223 / Production host: ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 45.29 % |
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Crystal grow | Temperature: 298 K / Method: microbatch / pH: 7.4 Details: 0.1M HEPES, 1.5M Sodium Citrate tribasic dihydrate pH 7.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Sep 8, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.65→25.83 Å / Num. obs: 7732 / % possible obs: 99.8 % / Redundancy: 3.9 % / Biso Wilson estimate: 31.1 Å2 / CC1/2: 0.98 / Net I/σ(I): 7.6 |
Reflection shell | Resolution: 2.65→2.745 Å / Num. unique obs: 763 / CC1/2: 0.88 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6IEO Resolution: 2.65→25.83 Å / SU ML: 0.25 / Cross valid method: FREE R-VALUE / σ(F): 1.97 / Phase error: 31.74 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.65→25.83 Å
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Refine LS restraints |
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LS refinement shell |
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