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- PDB-7vz3: Cryo-EM structure of Depo32, a Klebsiella phage depolymerase targ... -

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Basic information

Entry
Database: PDB / ID: 7vz3
TitleCryo-EM structure of Depo32, a Klebsiella phage depolymerase targets the K2 serotype K. pneumoniae
ComponentsDepolymerase
KeywordsHYDROLASE / Depolymerase / Klebsiella pneumoniae / K2 capsular type / Capsular polysaccharides
Function / homologybiological process involved in interaction with host / Pectin lyase fold/virulence factor / viral life cycle / virion component / Depolymerase
Function and homology information
Biological speciesKlebsiella phage GH-K3 (virus)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.46 Å
AuthorsCai, R. / Ren, Z. / Zhao, R. / Wang, X. / Guo, Z. / Du, R. / Han, W. / Ru, H. / Gu, J.
Funding support China, 3items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC)32072824 China
National Natural Science Foundation of China (NSFC)31872505 China
National Natural Science Foundation of China (NSFC)U19A2038 China
CitationJournal: Microbiol Spectr / Year: 2023
Title: Structural biology and functional features of phage-derived depolymerase Depo32 on with K2 serotype capsular polysaccharides.
Authors: Ruopeng Cai / Zhuolu Ren / Rihong Zhao / Yan Lu / Xinwu Wang / Zhimin Guo / Jinming Song / Wentao Xiang / Rui Du / Xiaokang Zhang / Wenyu Han / Heng Ru / Jingmin Gu /
Abstract: Hypervirulent with capsular polysaccharides (CPSs) causes severe nosocomial- and community-acquired infections. Phage-derived depolymerases can degrade CPSs from to attenuate bacterial virulence, ...Hypervirulent with capsular polysaccharides (CPSs) causes severe nosocomial- and community-acquired infections. Phage-derived depolymerases can degrade CPSs from to attenuate bacterial virulence, but their antimicrobial mechanisms and clinical potential are not well understood. In the present study, phage GH-K3-derived depolymerase Depo32 (encoded by gene ) was identified to exhibit high efficiency in specifically degrading the CPSs of K2 serotype . The cryo-electron microscopy structure of trimeric Depo32 at a resolution up to 2.32 Å revealed potential catalytic centers in the cleft of each of the two adjacent subunits. subjected to Depo32 became more sensitive to phagocytosis by RAW264.7 cells and activated the cells by the mitogen-activated protein kinase signaling pathway. In addition, intranasal inoculation with Depo32 (a single dose of 200 µg, 20 µg daily for 3 days, or in combination with gentamicin) rescued all C57BL/6J mice infected with a lethal dose of K7 without interference from its neutralizing antibody. In summary, this work elaborates on the mechanism by which Depo32 targets the degradation of K2 serotype CPSs and its potential as an antivirulence agent. IMPORTANCE Depolymerases specific to more than 20 serotypes of spp. have been identified, but most studies only evaluated the single-dose treatment of depolymerases with relatively simple clinical evaluation indices and did not reveal the anti-infection mechanism of these depolymerases in depth. On the basis of determining the biological characteristics, the structure of Depo32 was analyzed by cryo-electron microscopy, and the potential active center was further identified. In addition, the effects of Depo32 on macrophage phagocytosis, signaling pathway activation, and serum killing were revealed, and the efficacy of the depolymerase (single treatment, multiple treatments, or in combination with gentamicin) against acute pneumonia caused by was evaluated. Moreover, the roles of the active sites of Depo32 were also elucidated in the and studies. Therefore, through structural biology, cell biology, and experiments, this study demonstrated the mechanism by which Depo32 targets K2 serotype . infection.
History
DepositionNov 15, 2021Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0Aug 30, 2023Provider: repository / Type: Initial release
Revision 1.1Sep 11, 2024Group: Data collection / Database references / Category: citation / citation_author / em_admin
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Depolymerase
B: Depolymerase
C: Depolymerase


Theoretical massNumber of molelcules
Total (without water)302,8443
Polymers302,8443
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein Depolymerase


Mass: 100947.852 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Klebsiella phage GH-K3 (virus) / Gene: GHK3_32 / Plasmid: pET28a(+) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A3S7W7I3

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Tail spike protein Depo32 / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT
Molecular weightValue: 0.3 MDa / Experimental value: NO
Source (natural)Organism: Klebsiella phage 020009 (virus) / Strain: vB_KpnS_GH-K3
Source (recombinant)Organism: Escherichia coli (E. coli) / Strain: BL21(DE3) / Plasmid: pET28a(+)
Buffer solutionpH: 7.5
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMtris(hydroxymethyl)aminomethaneNH2C(CH2OH)31
2150 mMsodium chlorideNaCl1
30.5 mMtris(2-carboxyethyl)phosphineC9H15O6P1
SpecimenConc.: 0.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES / Details: This sample was monodisperse
Specimen supportGrid material: COPPER / Grid mesh size: 300 divisions/in. / Grid type: C-flat-1.2/1.3
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 285.15 K / Details: blot 3.5 or 4 seconds before plunging

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: DARK FIELD / Calibrated magnification: 81000 X / Nominal defocus max: 2000 nm / Nominal defocus min: 1500 nm / Cs: 2.7 mm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN
Image recordingAverage exposure time: 2.56 sec. / Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

SoftwareName: PHENIX / Version: (1.18.2_3874: ???) / Classification: refinement
EM software
IDNameCategory
1RELIONparticle selection
4RELIONCTF correction
7UCSF Chimeramodel fitting
9RELIONinitial Euler assignment
10RELIONfinal Euler assignment
11RELIONclassification
12RELION3D reconstruction
13PHENIXmodel refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C1 (asymmetric)
3D reconstructionResolution: 2.46 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 462388 / Symmetry type: POINT
Atomic model buildingProtocol: AB INITIO MODEL / Space: RECIPROCAL
RefinementResolution: 2.46→196.77 Å / SU ML: 0.35 / σ(F): 0.62 / Phase error: 39.3 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.3406 2015 7 %
Rwork0.3375 --
obs0.3375 3030756 99.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0117829
ELECTRON MICROSCOPYf_angle_d1.11724206
ELECTRON MICROSCOPYf_dihedral_angle_d16.282436
ELECTRON MICROSCOPYf_chiral_restr0.0642631
ELECTRON MICROSCOPYf_plane_restr0.0073191
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.46-2.520.55161440.5241216667ELECTRON MICROSCOPY100
2.52-2.590.4831440.5112216522ELECTRON MICROSCOPY100
2.59-2.670.48781320.5067215888ELECTRON MICROSCOPY100
2.67-2.750.53361440.5009217010ELECTRON MICROSCOPY100
2.75-2.850.50911680.4906215864ELECTRON MICROSCOPY100
2.85-2.960.53671320.4718216349ELECTRON MICROSCOPY100
2.96-3.10.51191440.4437216508ELECTRON MICROSCOPY100
3.1-3.260.36131200.3997216350ELECTRON MICROSCOPY100
3.26-3.470.3151680.3298216373ELECTRON MICROSCOPY100
3.47-3.730.24131440.27215790ELECTRON MICROSCOPY100
3.74-4.110.19751440.2111216703ELECTRON MICROSCOPY100
4.11-4.710.16321320.16216560ELECTRON MICROSCOPY100
4.71-5.930.17171560.1628216260ELECTRON MICROSCOPY100
5.93-196.770.19331430.1816215897ELECTRON MICROSCOPY100

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