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- PDB-7vwp: Structure of the flavin-dependent monooxygenase FlsO1 from the bi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7vwp | ||||||
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Title | Structure of the flavin-dependent monooxygenase FlsO1 from the biosynthesis of fluostatinsin | ||||||
![]() | FlsO1 | ||||||
![]() | OXIDOREDUCTASE / hydroxylation / benzo[b]-fluorene / epoxidation / Baeyer-Villiger Oxidation / prejadomycin | ||||||
Function / homology | FAD-binding domain / FAD binding domain / FAD binding / FAD/NAD(P)-binding domain superfamily / FLAVIN-ADENINE DINUCLEOTIDE / PHOSPHATE ION / FlsO1![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Zhang, Y. / Yang, C. / Zhang, L. / Zhang, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Biochemical and structural insights of multifunctional flavin-dependent monooxygenase FlsO1-catalyzed unexpected xanthone formation Authors: Yang, C. / Zhang, L. / Zhang, W. / Huang, C. / Zhu, Y. / Jiang, X. / Liu, W. / Zhao, M. / De, B.C. / Zhang, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 407.8 KB | Display | ![]() |
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PDB format | ![]() | 318.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 79.3 KB | Display | |
Data in CIF | ![]() | 113.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2qa1S S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 53276.980 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-PO4 / #3: Chemical | ChemComp-FAD / #4: Chemical | ChemComp-NA / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.64 Å3/Da / Density % sol: 53.47 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.02 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane, pH 6.5, 20 % w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: DECTRIS PILATUS 200K / Detector: PIXEL / Date: Mar 6, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54184 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→13.182 Å / Num. obs: 95967 / % possible obs: 98.9 % / Redundancy: 3.6 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 12.9 |
Reflection shell | Resolution: 2.3→2.34 Å / Rmerge(I) obs: 0.39 / Mean I/σ(I) obs: 2.8 / Num. unique obs: 4703 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2QA1 Resolution: 2.3→13.182 Å / Cor.coef. Fo:Fc: 0.938 / Cor.coef. Fo:Fc free: 0.893 / SU B: 8.819 / SU ML: 0.207 / Cross valid method: FREE R-VALUE / ESU R: 0.374 / ESU R Free: 0.265 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.263 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→13.182 Å
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Refine LS restraints |
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LS refinement shell |
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