PDB-7vec: Crystal structure of GABARAP complexed with the TEX264 LIR phosph...

Basic information

• Entry: Database: PDB / ID: 7vec
• Title: Crystal structure of GABARAP complexed with the TEX264 LIR phosphorylated at Ser271 and Ser272

Components

• Gamma-aminobutyric acid receptor-associated protein
• TEX264 phospho-LIR

• Keywords: SIGNALING PROTEIN / Autophagy / ER-phagy

Function / homology

Function:

positive regulation of protein K48-linked ubiquitination / regulation of Rac protein signal transduction / GABA receptor binding / cellular response to nitrogen starvation / phosphatidylethanolamine binding / TBC/RABGAPs / microtubule associated complex / Macroautophagy / beta-tubulin binding / axoneme / autophagosome membrane / autophagosome maturation / autophagosome assembly / extrinsic apoptotic signaling pathway via death domain receptors / smooth endoplasmic reticulum / autophagosome / protein targeting / sperm midpiece / microtubule cytoskeleton organization / protein transport / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / actin cytoskeleton / cytoplasmic vesicle / microtubule binding / chemical synaptic transmission / microtubule / lysosome / Golgi membrane / ubiquitin protein ligase binding / synapse / plasma membrane / cytosol

Domain/homology:

Autophagy protein Atg8 ubiquitin-like / Autophagy protein Atg8 ubiquitin like / Ubiquitin-like domain superfamily

Component:

Gamma-aminobutyric acid receptor-associated protein

• Biological species: Homo sapiens (human)
• Method: X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 3 Å
• Authors: Noda, N.N.

Funding support

Japan, 2items

Organization	Grant number	Country
Japan Science and Technology	JPMJCR20E3	Japan
Japan Society for the Promotion of Science (JSPS)	19H05707	Japan

• Citation: Journal: Embo Rep. / Year: 2022; Title: Phosphorylation by casein kinase 2 enhances the interaction between ER-phagy receptor TEX264 and ATG8 proteins.; Authors: Chino, H. / Yamasaki, A. / Ode, K.L. / Ueda, H.R. / Noda, N.N. / Mizushima, N.

History

Deposition	Sep 8, 2021	Deposition site: PDBJ / Processing site: PDBJ
Revision 1.0	Mar 30, 2022	Provider: repository / Type: Initial release
Revision 1.1	Oct 11, 2023	Group: Data collection / Database references Category: chem_comp_atom / chem_comp_bond / citation / citation_author Item: _citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2	Nov 29, 2023	Group: Refinement description / Category: pdbx_initial_refinement_model

Assembly

Deposited unit

A: Gamma-aminobutyric acid receptor-associated protein

B: Gamma-aminobutyric acid receptor-associated protein

C: Gamma-aminobutyric acid receptor-associated protein

D: Gamma-aminobutyric acid receptor-associated protein

E: Gamma-aminobutyric acid receptor-associated protein

F: Gamma-aminobutyric acid receptor-associated protein

G: Gamma-aminobutyric acid receptor-associated protein

H: Gamma-aminobutyric acid receptor-associated protein

I: Gamma-aminobutyric acid receptor-associated protein

J: Gamma-aminobutyric acid receptor-associated protein

K: Gamma-aminobutyric acid receptor-associated protein

L: Gamma-aminobutyric acid receptor-associated protein

M: TEX264 phospho-LIR

N: TEX264 phospho-LIR

O: TEX264 phospho-LIR

P: TEX264 phospho-LIR

Q: TEX264 phospho-LIR

R: TEX264 phospho-LIR

S: TEX264 phospho-LIR

T: TEX264 phospho-LIR

U: TEX264 phospho-LIR

V: TEX264 phospho-LIR

X: TEX264 phospho-LIR

Summary:

• 182 kDa, 23 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	181,680	23
Polymers	181,680	23
Non-polymers	0	0
Water	0	0

1

A: Gamma-aminobutyric acid receptor-associated protein

M: TEX264 phospho-LIR

Summary:

• defined by author&software
• Evidence: isothermal titration calorimetry
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1380 Å2
ΔGint	-11 kcal/mol
Surface area	6950 Å2
Method	PISA

2

B: Gamma-aminobutyric acid receptor-associated protein

N: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1390 Å2
ΔGint	-11 kcal/mol
Surface area	6910 Å2
Method	PISA

3

C: Gamma-aminobutyric acid receptor-associated protein

O: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1390 Å2
ΔGint	-11 kcal/mol
Surface area	6940 Å2
Method	PISA

4

D: Gamma-aminobutyric acid receptor-associated protein

P: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1370 Å2
ΔGint	-12 kcal/mol
Surface area	7020 Å2
Method	PISA

5

E: Gamma-aminobutyric acid receptor-associated protein

Q: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1330 Å2
ΔGint	-13 kcal/mol
Surface area	7300 Å2
Method	PISA

6

F: Gamma-aminobutyric acid receptor-associated protein

R: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1380 Å2
ΔGint	-9 kcal/mol
Surface area	7020 Å2
Method	PISA

7

G: Gamma-aminobutyric acid receptor-associated protein

S: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1400 Å2
ΔGint	-11 kcal/mol
Surface area	7130 Å2
Method	PISA

8

H: Gamma-aminobutyric acid receptor-associated protein

T: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1280 Å2
ΔGint	-13 kcal/mol
Surface area	7240 Å2
Method	PISA

9

I: Gamma-aminobutyric acid receptor-associated protein

U: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1150 Å2
ΔGint	-10 kcal/mol
Surface area	7000 Å2
Method	PISA

10

J: Gamma-aminobutyric acid receptor-associated protein

V: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	1350 Å2
ΔGint	-9 kcal/mol
Surface area	7180 Å2
Method	PISA

11

K: Gamma-aminobutyric acid receptor-associated protein

Summary:

• defined by author&software
• 14 kDa, 1 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	13,983	1
Polymers	13,983	1
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	0 Å2
ΔGint	0 kcal/mol
Surface area	6870 Å2
Method	PISA

12

L: Gamma-aminobutyric acid receptor-associated protein

X: TEX264 phospho-LIR

Summary:

• defined by author&software
• 15.2 kDa, 2 polymers

Component details:

	Theoretical mass	Number of molelcules
Total (without water)	15,245	2
Polymers	15,245	2
Non-polymers	0	0
Water	0

Symmetry operations:

Type	Name	Symmetry operation	Number
identity operation	1_555	x,y,z	1

Calculated values:

Buried area	930 Å2
ΔGint	-6 kcal/mol
Surface area	6790 Å2
Method	PISA

Unit cell

Length a, b, c (Å)	200.480, 201.383, 211.816
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	22
Space group name H-M	F222
Symmetry operation	#1: x,y,z #2: x,-y,-z #3: -x,y,-z #4: -x,-y,z #5: x,y+1/2,z+1/2 #6: x,-y+1/2,-z+1/2 #7: -x,y+1/2,-z+1/2 #8: -x,-y+1/2,z+1/2 #9: x+1/2,y,z+1/2 #10: x+1/2,-y,-z+1/2 #11: -x+1/2,y,-z+1/2 #12: -x+1/2,-y,z+1/2 #13: x+1/2,y+1/2,z #14: x+1/2,-y+1/2,-z #15: -x+1/2,y+1/2,-z #16: -x+1/2,-y+1/2,z

Components

#1: Protein

A B C D E F G H I J K L
Gamma-aminobutyric acid receptor-associated protein / GABA(A) receptor-associated protein / MM46

Details:

Mass: 13983.056 Da / Num. of mol.: 12
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: GABARAP, FLC3B, HT004 / Production host: Escherichia coli (E. coli) / References: UniProt: O95166

#2: Protein/peptide

M N O P Q R S T U V X
TEX264 phospho-LIR

Details:

Mass: 1262.107 Da / Num. of mol.: 11 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)

• Has ligand of interest: N

Experimental details

Experiment

• Experiment: Method: X-RAY DIFFRACTION / Number of used crystals: 1

Sample preparation

• Crystal: Density Matthews: 3 ų/Da / Density % sol: 59.01 %
• Crystal grow: Temperature: 293 K / Method: vapor diffusion, sitting drop; Details: 30% PEG 4000, 0.2M ammonium acetate, 0.1M bis-Tris pH 5.6

Data collection

• Diffraction: Mean temperature: 100 K / Serial crystal experiment: N
• Diffraction source: Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 HF / Wavelength: 1.54178 Å
• Detector: Type: RIGAKU HyPix-6000HE / Detector: PIXEL / Date: Jun 4, 2021
• Radiation: Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
• Radiation wavelength: Wavelength: 1.54178 Å / Relative weight: 1
• Reflection: Resolution: 3→30.48 Å / Num. obs: 42776 / % possible obs: 99.9 % / Redundancy: 8.7 % / CC_1/2: 0.964 / Net I/σ(I): 6.5
• Reflection shell: Resolution: 3→3.11 Å / Redundancy: 8 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 4477 / CC_1/2: 0.608 / % possible all: 100

Processing

Software

Name	Version	Classification
PHENIX	(1.17.1_3660: ???)	refinement
CrysalisPro	1.171.40.14a	data reduction
Aimless		data scaling
MrBUMP		phasing

Refinement

Method to determine structure: MOLECULAR REPLACEMENT
Starting model: 5YIR

5yir

Resolution: 3→28.89 Å / SU ML: 0.43 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 28.35 / Stereochemistry target values: ML

	Rfactor	Num. reflection	% reflection
Rfree	0.3007	2233	5.34 %
Rwork	0.257	-	-
obs	0.2594	41787	97.65 %

• Solvent computation: Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 3→28.89 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	12376	0	0	0	12376

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.003	12703
X-RAY DIFFRACTION	f_angle_d	0.671	17160
X-RAY DIFFRACTION	f_dihedral_angle_d	19.192	4791
X-RAY DIFFRACTION	f_chiral_restr	0.05	1764
X-RAY DIFFRACTION	f_plane_restr	0.005	2204

LS refinement shell

Resolution (Å)	Rfactor Rfree	Num. reflection Rfree	Rfactor Rwork	Num. reflection Rwork	Refine-ID	% reflection obs (%)
3-3.07	0.4133	155	0.3736	2477	X-RAY DIFFRACTION	99
3.07-3.14	0.3287	100	0.3174	2525	X-RAY DIFFRACTION	100
3.14-3.21	0.3212	175	0.2761	2495	X-RAY DIFFRACTION	100
3.21-3.3	0.2971	155	0.2688	2502	X-RAY DIFFRACTION	100
3.3-3.4	0.3041	152	0.2593	2524	X-RAY DIFFRACTION	100
3.4-3.51	0.3122	134	0.2673	2468	X-RAY DIFFRACTION	100
3.51-3.63	0.5603	118	0.4615	1912	X-RAY DIFFRACTION	76
3.63-3.78	0.5289	128	0.4702	2307	X-RAY DIFFRACTION	92
3.78-3.95	0.3745	142	0.3041	2470	X-RAY DIFFRACTION	98
3.95-4.16	0.2406	186	0.2365	2449	X-RAY DIFFRACTION	100
4.16-4.42	0.2311	164	0.193	2517	X-RAY DIFFRACTION	100
4.42-4.76	0.2131	93	0.1669	2572	X-RAY DIFFRACTION	100
4.76-5.23	0.1887	160	0.1612	2531	X-RAY DIFFRACTION	100
5.23-5.98	0.218	131	0.1783	2554	X-RAY DIFFRACTION	100
5.98-7.52	0.2213	116	0.1904	2604	X-RAY DIFFRACTION	100
7.52-28.89	0.2082	124	0.1668	2647	X-RAY DIFFRACTION	99

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°2)	L12 (°2)	L13 (°2)	L22 (°2)	L23 (°2)	L33 (°2)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å2)	T12 (Å2)	T13 (Å2)	T22 (Å2)	T23 (Å2)	T33 (Å2)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	0.0024	-0.0017	0.0029	0.0012	0.0024	-0.0039	-0.0477	0.029	-0.0024	0.0166	-0.0135	0.0106	0.0466	0.017	-0	0.1627	-0.0085	0.0549	-0.0115	-0.1114	0.1454	-62.0575	35.5256	-17.0322
2	0.003	0.0056	0.0048	0.0004	0.0043	0.0006	-0.0133	0.0362	-0.0299	0.0349	0.0325	-0.0432	-0.0326	-0.0247	0	-0.0438	-0.1971	-0.3231	-0.1536	-0.3158	0.0607	-68.4343	24.2741	-22.7653
3	0.0192	-0.041	-0.0288	0.0215	0.0207	-0.0137	-0.2009	0.225	-0.1224	-0.1474	-0.0585	-0.0969	0.1229	0.2402	0	-0.4923	0.0132	0.2172	-0.7383	-0.4358	-0.1733	-50.2869	26.4453	-20.1825
4	0.0021	-0.0003	-0.001	0.0001	0.0006	-0.0003	-0.0056	-0.0032	0.0063	0.0133	0.0206	0.008	-0.0057	0.0152	-0	0.1214	0.1588	0.0173	0.0624	-0.1396	0.0991	-44.6197	34.9764	-9.907
5	-0.0002	0.0011	-0.0024	0.0013	0.0017	-0.0021	0.0258	-0.0222	0.0097	0.01	0.0311	0.0024	-0.0007	-0.0031	-0	0.128	0.1888	0.3172	0.0417	-0.3778	0.1368	-36.529	10.6498	-18.2483
6	0.0003	0.0047	-0.0054	0.0042	0.0005	-0.0019	-0.0582	0.0137	0.0391	-0.0389	-0.0419	0.039	-0.0641	0.0267	0	0.0847	0.1385	0.311	-0.092	-0.375	0.0298	-30.9351	18.1056	-29.4378
7	-0.0023	0.0039	0.0028	0.0091	0.0054	0.0047	0.0435	0.0162	0.0108	0.0666	-0.0624	-0.053	0.0446	0.0097	-0	0.1869	-0.0531	0.0966	-0.0245	-0.1549	-0.1159	-28.6918	6.4713	-29.6655
8	0.0101	0.0093	0.0077	0.0017	-0.0051	-0.003	0.0284	0.0738	0.0287	0.0389	-0.0803	-0.0442	0.078	-0.0583	-0	-0.0352	-0.1418	-0.1755	0.096	-0.1479	-0.1252	-29.2266	0.6526	-23.9337
9	0	-0.0028	0.0028	0.0016	-0.0006	-0.0012	-0.0223	-0.041	-0.0343	-0.04	-0.0348	0.0211	0.0451	0.0317	0	-0.0654	-0.2027	-0.0869	0.1619	-0.0798	-0.2191	-27.0309	-6.5541	-27.3238
10	-0.0074	-0.008	0.0061	0.002	0.0053	-0.0011	0.1177	0.0058	0.1033	0.019	-0.0203	0.0685	0.0755	0.0121	-0	-0.0846	-0.2643	-0.2051	-0.1117	-0.4838	-0.4386	-35.2523	-7.7216	-26.4407
11	0.0007	0.0002	-0.0006	0.001	0.002	0.0023	0.0008	0.0126	0.0034	-0.0156	0.0157	0.0271	-0.0138	0.0106	0	-0.1213	0.2066	0.118	0.2083	-0.245	-0.0093	-37.16	4.1099	-36.4437
12	0.0004	0.0037	0.006	0.0017	0.0018	0.0023	0.1124	-0.0393	0.0396	0.0519	0.016	-0.0192	0.0408	-0.0687	-0	0.0795	-0.0183	0.0536	0.0928	-0.016	0.0329	-36.5239	7.4904	-27.3722
13	0.0009	-0.002	0.0003	0.0037	0.002	0.001	-0.0043	-0.0068	0.0036	0.0119	-0.0143	-0.0028	0.0054	-0.0145	0	0.0778	-0.0388	0.1928	0.0136	-0.2413	0.0777	-41.941	-6.9942	-18.9815
14	0.0012	0.0028	0.0028	-0.0015	0.0004	0.003	0.013	0.014	0.0027	-0.0253	0.0012	-0.0168	0.0107	-0.0407	0	-0.1744	0.0164	-0.1502	0.1247	-0.0658	0.0714	-32.4687	-13.0575	-43.8317
15	-0.0009	-0.0007	-0.0026	-0.0005	-0.0014	0.0014	0.0057	-0.0119	-0.0831	0.0609	-0.048	0.0077	-0.002	-0.0365	-0	-0.144	0.3653	0.2077	-0.1644	-0.1333	0.0041	-21.7892	-19.1909	-35.4455
16	-0.0002	-0.0036	0.01	0.0043	0.0075	0.0123	0.033	0.0521	-0.0222	0.0675	0.0607	-0.0982	0.0413	-0.067	-0	0.072	0.0036	-0.0529	0.1043	-0.0428	0.0718	-24.353	-20.9366	-50.8984
17	0.0012	-0.0063	0.0007	0.0022	0.0045	0.0003	0.0495	0.0207	-0.0387	-0.0313	0.128	0.031	0.0512	0.0412	-0	-0.1507	-0.0797	-0.1727	-0.0016	-0.1383	-0.1322	-24.6279	-20.8646	-62.034
18	0.0022	-0.0022	-0.0061	0.0036	-0.0033	0.0006	-0.0452	0.0302	0.0315	-0.0604	0.0376	-0.0097	-0.0013	0.0923	0	0.102	-0.0445	0.1086	0.1347	-0.0025	-0.0895	-15.7626	-11.8634	-53.03
19	0.0111	0.007	-0.0011	-0.0043	-0.0142	0.0017	-0.086	0.0779	0.0382	0.121	-0.0423	-0.0318	-0.0153	-0.0782	0	-0.2805	0.2292	-0.4562	-0.0767	0.2106	-0.2691	-23.0137	-13.5396	-46.1759
20	0.0002	0.0005	-0.0006	0.0015	-0.0006	-0.0007	0.008	-0.0033	0.0354	-0.0057	-0.0127	0.009	0.0179	-0.0164	0	0.0841	-0.0602	0.099	0.2104	-0.008	0.1338	-30.0319	-7.7215	-61.1912
21	-0.0003	-0.0036	-0.0053	0.0002	-0.0025	0.0031	0.0258	0.0398	-0.0388	-0.02	0.0582	-0.0333	0.0179	0.0195	0	-0.0581	0.0661	0.1877	0.0983	0.0215	-0.0756	-16.5055	55.518	-32.1112
22	-0.0018	0.0009	0.0026	-0.0075	-0.0048	-0.0006	-0.1004	0.0512	-0.0198	0.0854	0.0929	-0.072	-0.025	0.0242	0	-0.3006	-0.1645	0.1236	0.0609	0.2777	0.0196	-20.4871	63.9608	-20.7548
23	0.006	-0.0039	0.0074	0.0003	-0.0014	0.0012	-0.0459	0.0372	-0.0615	0.0323	0.0386	-0.0534	-0.0359	-0.031	-0	-0.0003	0.0681	-0.1311	0.0073	0.2316	-0.0695	-23.2472	52.8491	-19.5988
24	0.0007	0.0037	-0.0019	0.0036	-0.0032	-0.0008	0.0229	0.0064	0.0046	-0.0114	0.045	0.0071	-0.0203	0.032	0	0.1535	0.0799	0.0202	0.2291	0.1248	0.2098	-21.7851	43.9178	-32.9327
25	0.0042	0.0008	-0.0023	-0.0008	-0.0028	0.0005	-0.0491	-0.0178	-0.0614	-0.0075	-0.0141	-0.0018	0.0176	0.0124	0	-0.1491	-0.0335	0.101	0.2174	-0.0305	0.0204	-24.873	49.3788	-16.0365
26	-0.0002	0.0025	0.0051	-0.0014	-0.0034	-0.0023	-0.0149	0.0239	-0.0247	0.0261	-0.0524	0.006	0.0466	-0.0085	-0	-0.0036	-0.0473	0.069	0.0758	0.0578	0.0953	-25.7162	39.8803	-20.7904
27	-0.0001	0.0044	-0.0018	-0.0014	-0.0009	-0.0002	0.0044	0.0114	-0.0122	-0.0373	-0.013	0.0116	0.0459	-0.0716	0	-0.0715	0.0309	0.3115	-0.0044	0.1329	0.041	-21.9564	34.4435	-26.2041
28	0.0007	-0.0058	-0.0007	0.001	-0.0038	0.0008	0.0135	-0.0165	0.0039	0.0756	0.0598	0.0033	0.0202	0.0019	0	-0.1325	0.2477	0.0434	-0.028	0.0907	0.2749	-12.5195	42.6074	-17.49
29	0.0005	-0.0007	0.0013	0.0002	-0.0021	-0.0014	0.0194	-0.0257	-0.0207	0.0341	0.0266	0.0182	0.0431	0.0071	0	0.0816	0.1999	-0.2269	0.1008	0.1328	-0.2564	-14.6327	50.6988	-13.5579
30	-0.0026	0.0024	0.0007	-0	-0.0046	0.0062	-0.0253	0.0342	0.1075	0.0123	-0.0385	-0.0173	0.0785	0.0536	-0	0.0634	0.1178	0.0424	-0.2344	-0.0186	0.0211	-15.6388	53.1496	-22.7667
31	0.0002	0.0004	0.0001	-0.0007	-0.0002	-0.0007	0.0076	0.0158	0.0032	-0.0118	0.0172	-0.0053	-0.0088	0.0317	-0	0.1786	0.1287	0.2208	0.0636	-0.0252	-0.0552	-11.5558	37.8509	-30.1935
32	-0.0011	0.0034	0.0017	-0.0009	0.0003	-0.001	0.0469	0.0156	-0.0197	0.0036	0.0137	0.0467	-0.0093	0.0003	0	0.1393	0.0285	0.0013	0.0239	-0.047	0.0664	-72.419	-6.4687	-19.9161
33	-0.001	0.0046	0.0029	-0.0001	0.0018	0.0026	0.026	0.0402	0.0109	0.0771	0.0416	0.0204	-0.0023	-0.0153	0	-0.171	-0.0653	-0.2973	0.2139	-0.1808	-0.0318	-77.3005	-11.7635	-32.5729
34	-0.0061	0.0006	0.0042	0.0069	0.002	-0.0003	0.0107	0.0783	0.002	0.032	-0.0219	-0.0162	-0.0126	-0.0213	-0	-0.2464	-0.3058	-0.2081	-0.1579	-0.4583	0.0643	-79.2637	-0.3374	-31.1476
35	-0.0136	0.0091	0.004	0.0046	0.002	-0.003	-0.0266	-0.0926	-0.077	-0.043	-0.0951	0.1549	-0.0761	-0.0015	-0	0.1126	-0.1945	-0.1826	0.046	-0.3337	0.1216	-78.2649	5.1988	-23.4285
36	-0.0112	0	-0.0154	-0.0124	0.0107	0.003	-0.0596	-0.0827	0.0751	0.0638	-0.0859	0.099	-0.0819	0.0273	-0	-0.5379	-0.3034	-0.6581	-0.2019	-0.5171	-0.6461	-75.8126	12.7379	-25.8938
37	-0.0075	0.0069	0.0031	0.0021	0.0055	-0.008	-0.0246	-0.0034	-0.0214	0.0709	0.1004	0.0591	-0.0317	-0.003	-0	-0.5632	-0.5038	-0.7368	-0.3652	-0.5805	-0.3908	-71.1752	0.0541	-31.6894
38	0.0006	-0.0001	-0.0011	0.0003	-0.0007	0.0009	-0.0218	0.0005	-0.008	0.0094	-0.0277	0.0107	0.0045	0.0024	-0	0.1411	0.0396	0.0015	0.101	0.1432	0.0563	-66.4628	11.2103	-17.6994
39	-0.0001	0.0016	0.0013	-0.0008	0.0016	-0.0015	0.0098	-0.0277	-0.0282	0.0206	0.0162	0.0183	0.0028	0.0046	0	-0.1221	-0.1872	-0.4824	-0.0705	-0.2516	-0.145	-46.6051	-31.0806	-19.3197
40	0.0059	0.0009	-0.0057	0.0026	-0.0002	0.0018	-0.0904	-0.0411	-0.0179	-0.0508	-0.0116	0.0568	-0.0258	0.0394	-0	0.0345	-0.0111	0.0718	0.0403	0.0184	0.0146	-39.5144	-19.6369	-25.1396
41	0.0054	-0.0015	0.0073	-0.0005	-0.0036	0.0018	-0.005	0.0408	0.0498	-0.0298	-0.0161	-0.0016	-0.0513	-0.0118	0	-0.1684	-0.3252	-0.0866	-0.0622	0.0172	0.0976	-51.2768	-19.5107	-26.8649
42	0.0018	0.0052	0.0017	0.0017	0.0043	0.0018	0.0554	0.0088	-0.04	0.0345	0.0363	-0.0246	0.021	0.0144	0	0.228	-0.1434	0.0194	0.226	0.04	0.0885	-59.2098	-33.6557	-22.889
43	0.0038	-0.0058	0.003	-0.002	-0.0012	0.0022	-0.0909	0.0059	0.0766	0.0115	0.0599	0.0301	0.0019	0.0554	-0	-0.1759	0.0216	-0.4861	0.0545	0.0491	-0.127	-60.672	-19.4037	-27.8621
44	0.0066	-0.0113	0.0087	0.0046	-0.0008	0.0028	0.0096	-0.0492	0.1743	-0.0131	0.0594	-0.0508	-0.0105	-0.0022	-0	0.2159	0.0498	-0.1432	0.0802	0.0774	-0.2369	-64.2477	-22.2612	-18.9984
45	0.0027	-0.0002	0.002	-0.0006	0.0018	0.0031	-0.0184	-0.0182	0.057	0.0441	0.0159	0.1052	-0.0077	0.0189	-0	0.0129	-0.057	-0.0866	0.0878	-0.059	0.194	-49.0014	-15.692	-18.4946
46	0.0052	0.0053	-0.0028	0.0017	0.003	0	-0.0808	0.0335	0.0021	0.0164	-0.0825	0.0582	0.0176	0.0361	-0	-0.0122	-0.0761	-0.031	-0.0324	-0.2013	-0.0005	-56.1543	-25.129	-19.6217
47	0.0045	0.0018	0.0039	0.0023	0.0025	0.0062	-0.0078	0.0095	-0.015	0.0157	0.0061	0	-0.0026	-0.0051	0	0.2433	0.0027	0.0608	0.2916	-0.0749	0.2755	-66.1014	-29.85	-13.0237
48	0.0013	0.0034	0.0058	-0.0051	0.01	-0.0115	0.0695	-0.0594	0.0222	0.0051	0.0241	-0.0603	-0.0578	0.1247	0	-0.2423	0.1261	0.2418	-0.5604	-0.0954	0.0105	-26.9743	30.2791	-8.7783
49	0.0001	0.0192	0.0006	0.0179	-0.0128	0.0161	0.1007	-0.0176	0.0302	-0.0991	0.174	0.1352	0.0026	0.0608	-0	0.0812	0.0153	0.0088	-0.041	0.013	0.1917	-29.0192	26.2209	6.305
50	-0.0025	-0.0013	0.0013	0.0007	-0.0009	0.0011	-0.0187	-0.0657	-0.0541	0.0314	-0.0155	-0.0065	0.0365	0.0095	0	0.017	0.046	0.143	-0.2221	-0.3867	0.0492	-24.6978	28.3148	17.2528
51	-0.0175	-0.01	0.0147	0.0028	-0.0066	-0.0008	-0.1554	0.0555	-0.0074	-0.1144	-0.0466	0.0684	0.1335	-0.0506	-0	-0.1068	0.425	0.2534	-0.6028	-0.37	-0.1834	-31.5487	35.928	4.4085
52	-0.0011	-0.0011	-0.0001	0.0019	0.0012	0.0006	0.0053	0.0117	0.0297	-0.0067	-0.0397	0.0257	-0.0013	-0.0151	-0	0.1237	-0.0101	0.1021	-0.0507	0.1005	0.0887	-21.6615	-42.18	-37.8767
53	0.0028	0.0035	-0.0032	-0.0001	0.0002	-0.0014	0.0357	-0.0323	0.0188	-0.0186	0.0314	-0.0173	-0.0137	-0.0631	0	0.1076	-0.1387	-0.1292	-0.1839	-0.1593	0.1775	-27.8588	-36.4687	-25.982
54	-0.0112	-0.0078	0.0007	-0.0041	0.0093	-0.0053	0.0444	-0.0332	0.0501	-0.0487	-0.1502	0.0097	0.0033	-0.069	0	-0.3984	0.4087	0.2718	-0.2715	-0.2044	-0.06	-27.3175	-51.8824	-31.2775
55	0.0032	0.0099	-0.0005	-0.005	0.0095	-0.0027	0.0004	0.0351	-0.0836	-0.086	0.0402	0.0766	0.0261	-0.0967	0	-0.5107	-0.2993	0.5133	-0.4139	0.2874	-0.133	-26.2723	-62.5644	-32.8263
56	0.0009	0.0022	-0.0021	0.001	0.0023	-0.0007	-0.1187	-0.0315	0.0291	0.037	-0.0817	-0.0153	-0.0147	0.0303	0	-0.1792	-0.2707	0.0216	-0.1109	-0.0065	0.0639	-18.1951	-53.558	-22.9025
57	0.0068	-0.0083	0.0124	0.0057	-0.0123	0.0026	0.0381	-0.0153	0.0657	-0.0516	-0.029	-0.0309	0.067	0.1106	0	0.0717	-0.0379	-0.0122	-0.0541	-0.0362	0.0139	-18.8445	-50.0868	-31.803
58	0.0015	0.001	-0.0006	0.0013	-0.0015	-0.0011	0.0157	-0.0006	0.0043	0.0078	-0.0002	0.0162	0.0025	-0.0116	-0	0.226	0.0004	0.1617	0.3011	-0.0265	0.2212	-8.502	14.3465	-29.4047
59	0.0021	-0.0054	-0.001	0.0024	0.0002	0.0012	-0.045	0.0281	-0.0906	0.0529	0.0591	0.0316	-0.0048	-0.0535	-0	0.071	0.045	0.0287	0.0859	-0.0435	0.2773	-14.2555	25.9686	-23.2413
60	0.0074	0.0002	0.001	0.0019	-0.0046	0.0055	-0.0752	-0.0292	0.0104	0.023	-0.0215	-0.0263	-0.0173	-0.0337	-0	0.0384	0.3778	0.1588	-0.0636	0.0244	0.072	-2.543	25.163	-22.307
61	0.0038	-0.0053	-0.0063	-0.0011	0.0061	-0.0051	0.03	-0.0914	-0.0512	0.0412	0.0252	0.0099	-0.0142	0.0026	0	0.2627	0.0958	0.07	0.2637	0.0401	0.2065	2.7939	18.4089	-23.887
62	0.0083	-0.0055	-0.0172	-0.0153	-0.0099	0.0025	-0.107	-0.0779	0.05	0.0659	-0.0647	-0.0504	0.0183	-0.0148	0	-0.7466	0.3174	0.7529	-0.0788	-0.2549	-0.4978	10.2728	20.9335	-27.2483
63	-0.0002	-0.0008	-0.0021	-0.0007	0.0012	0.0008	-0.0273	-0.0186	-0.0062	-0.0031	-0.0268	0.0148	-0.0044	0.0209	-0	0.0315	0.1102	0.2614	0.161	-0.0779	0.1897	-0.4302	31.5872	-30.4524
64	0.0012	0.0028	-0.0087	0.0024	-0.012	-0.0033	-0.0291	-0.0464	-0.0133	-0.024	-0.07	-0.0135	-0.0169	0.0173	0	-0.2683	0.3541	0.352	-0.3634	0.1287	0.1506	-4.4981	23.0315	-29.9989
65	-0.001	0.0009	-0.0002	0.0009	-0.0012	0.0011	-0.0095	-0.0142	-0.0187	0.0143	-0.0075	0.0262	0.0189	0.0258	-0	0.1588	0.1047	-0.0137	0.0329	0.0759	0.0367	8.5598	13.4773	-37.0969
66	0.0049	0.0068	0.01	-0.0055	-0.0042	0.0034	0.1097	0.0096	-0.0103	-0.0736	0.0929	-0.1198	-0.0344	0.0779	0	-0.0552	0.2041	-0.2512	-0.6182	0.4728	-0.08	11.212	24.1554	-70.3054
67	0.0135	0.0045	0.0308	-0.0026	-0.0056	0.0109	0.1193	-0.0276	-0.0473	-0.0868	0.0987	0.0214	-0.0675	-0.0053	0	-0.4284	0.3928	-0.6137	-0.2482	0.4382	-0.2601	-1.2267	26.0906	-74.5314
68	-0.001	-0.0051	0.0069	-0.0051	0.0036	-0.0038	-0.0649	0.0446	0.0025	0.0492	0.0065	0.0006	0.0273	-0.0123	0	-0.3393	0.162	-0.3179	-0.2573	0.4095	-0.1486	-9.8837	29.0207	-77.1724
69	-0.0017	0.0048	0.0056	0.0037	-0.0091	-0.001	0.0346	-0.0381	0.0512	-0.0373	0.0591	0.0115	0.016	0.0336	0	-0.0172	0.0243	-0.0823	-0.0433	0.547	-0.0105	-15.3353	24.679	-72.6293
70	-0.0015	0.0088	-0.0003	-0.0051	0.0031	-0.001	0.0002	-0.1438	0.2343	0.0664	0.0115	0.0334	-0.0781	-0.1021	-0	-0.4058	0.4318	-0.2147	-0.176	0.219	-0.0134	0.2894	32.2037	-66.5395
71	0.0033	-0.0008	0.0006	0.0001	-0.0007	0.0017	0.0158	0.0029	-0.0154	0.0084	0.0074	0.0046	0.0036	-0.0056	-0	0.3477	-0.0129	-0.0529	0.4519	0.2263	0.2377	-11.7433	21.8925	-61.6337
72	0.002	0.0006	0.0007	-0.0012	0.0004	0.0014	0.0263	-0.0266	-0.0243	0.0118	0.0058	-0.0058	0.004	-0.0151	-0	0.5209	-0.0023	0.0398	0.6418	0.2075	0.4063	-88.2726	31.2746	9.2102
73	-0.003	-0.0092	-0.001	-0.0039	-0.0083	0.0023	0.044	-0.0259	-0.0345	0.0271	0.0006	-0.04	-0.0019	-0.0709	-0	0.2271	-0.1398	-0.0088	0.4956	0.1916	-0.0477	-76.9411	24.3798	15.3928
74	-0.0027	-0.0077	-0.0016	-0.0018	0.0077	0.0089	0.0283	-0.0133	-0.0615	0.0384	-0.0757	-0.0599	-0.002	-0.0387	-0	0.3248	0.23	-0.0124	0.3632	0.0096	0.333	-77.6467	22.8424	3.9067
75	0.0018	0.01	-0.0008	0.0056	0.0021	0.0087	0.1239	0.0198	0.0268	-0.0021	0.0722	0.0571	0.1334	-0.1149	0	0.4615	0.0893	-0.0268	0.1416	0.0296	-0.0707	-82.8781	23.7262	-4.8254
76	0.0002	0.013	-0.0016	-0.0096	-0.0164	0.0095	-0.0861	-0.0193	-0.0033	0.046	0.0353	0.0051	0.1479	-0.0638	-0	0.1818	-0.2172	0.1388	0.1241	0.0171	-0.4923	-81.8373	31.2476	-9.442
77	-0.0012	0.0014	0.0058	0.0015	0.0005	0.0091	-0.055	-0.0272	-0.0498	-0.0148	0.0505	-0.0219	0.0957	0.0039	-0	0.4091	0.116	0.0394	0.3653	0.0465	0.0625	-78.1988	32.3749	1.3734
78	0.0011	0.0001	0.0005	-0.0006	-0.0013	0.0017	-0.0075	-0.0044	0.0088	-0.0099	-0.0087	0.0097	-0.0245	-0.0057	0	0.3557	-0.0609	0.1073	0.5557	0.0236	0.2644	-35.8471	24.1639	-59.7706
79	0.0051	-0.0067	-0.0006	0.0141	-0.0068	0.0003	-0.0537	0.0938	0.1113	-0.0083	0.0448	-0.0987	-0.0693	0.0181	-0	0.2701	-0.0689	0.0471	0.3384	0.0283	0.4044	-27.8763	29.3535	-57.0098
80	-0.0056	0.0058	-0.0009	0.0053	-0.0012	0.0055	-0.0487	0.0124	0.039	0.0676	-0.0285	0.1	-0.0456	0.0101	-0	0.5854	-0.0552	0.0202	0.212	0.0441	0.4468	-28.4327	29.5483	-41.8879
81	0.0046	0.0017	-0.0038	-0.0026	-0.0034	0.0056	-0.1281	-0.0023	-0.0145	-0.0009	-0.0949	0.017	-0.0106	0.0213	-0	0.1422	0.0922	0.4288	0.5439	0.2008	-0.0595	-23.8614	21.2071	-51.346
82	0.001	-0.0002	0	0.0006	0	0.0002	-0.0022	0.0062	0.0042	0.0111	0.0054	-0.0047	0.0008	-0.0078	0	0.5482	-0.026	0.0139	0.5249	-0.0646	0.5132	-36.995	16.4995	-42.7139
83	0.0007	-0.0016	0.0013	0.0003	-0.0006	0.002	0.0236	0.0277	-0.0025	-0.0029	0.0492	0.0183	0.0202	-0.0045	0	0.1328	0.0809	0.087	0.2006	-0.0176	0.14	-52.6668	24.354	-31.1768
84	-0.0005	0.0004	0.0008	0.0002	-0.0003	0.001	-0.0068	0.0065	0.0339	-0.0222	-0.013	0.0016	0.0257	0.0102	-0	0.0092	0.0702	-0.0274	0.1333	-0.0716	0.0843	-21.4375	3.1676	-29.6896
85	0.0005	0.0012	-0.0007	0.0024	0.003	-0.0001	0.0261	0.0083	-0.0191	-0.0086	-0.0192	-0.0228	0.0566	0.0139	0	0.2495	-0.0866	0.172	0.0761	-0.3586	-0.0232	-20.0898	-29.555	-51.7123
86	0.002	0.0018	0.0046	0.0006	-0.0026	0.001	0.0135	0.0192	0.0022	0.0122	0.0336	-0.0117	0.0068	-0.0017	-0	0.1209	-0.1789	-0.1142	0.0066	0.2008	0.1849	-31.5688	48.8292	-17.5869
87	-0.0026	-0.0023	0.0008	-0.0004	0.0007	-0.0004	0.0047	0.0149	0.0135	0.0115	0.0189	0.0179	0.0037	-0.0126	0	0.2105	0.0194	-0.0586	0.1925	-0.1569	0.2135	-86.7992	4.1242	-32.0432
88	-0.0003	-0.0004	-0.0005	0	-0.0007	0.0027	-0.0206	-0.01	0.0053	0.0038	-0.0259	-0.0072	-0.0285	0.0265	-0	0.1545	-0.181	0.0214	0.2265	0.0831	-0.0657	-55.1141	-19.3411	-33.6871
89	0.0008	0.0016	-0.0009	0.0011	0.001	0.0042	-0.0089	0.0049	0.009	-0.0321	0.0083	0.0048	-0.0141	-0.001	-0	0.1792	-0.0047	-0.032	0.1821	0.0595	0.1477	-32.7778	19.1777	3.0155
90	0.0015	-0.0021	-0.0008	-0.0003	-0	-0.0001	-0.0447	-0.046	-0.018	-0.0102	-0.0309	0.0475	0.0006	-0.0213	0	0.1122	-0.0814	0.0989	0.1227	0.0223	0.0952	-35.818	-53.3631	-26.1159
91	-0.0005	-0.0002	-0.0011	0.0009	0.0004	0.0023	0.0218	-0.0048	0.0023	-0.0104	0.0312	0.0026	-0.0006	0.0177	-0	0.5365	0.0536	0.1246	0.5295	-0.0029	0.3975	0.3123	23.911	-15.3528
92	0.0015	0.0014	0.0005	-0.0002	0.0005	0.0008	-0.0204	0.0038	0.0187	-0.0349	-0.0052	0.047	-0.0269	0.0108	-0	0.1075	-0.0798	-0.1981	0.1635	-0.0295	0.1618	0.1492	29.5742	-82.5842
93	-0.0002	0.0009	0.0002	0.0007	-0.0009	0.0003	-0.0124	0.0079	-0.0023	0.0078	-0.0025	-0.0041	0.0036	0.0054	-0	0.5146	-0.0313	0.0525	0.4543	-0.0501	0.4748	-25.9385	37.595	-52.5642

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 1 through 10 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 11 through 24 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 25 through 110 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 111 through 116 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 1 through 10 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 11 through 24 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 25 through 35 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 36 through 56 )
9	X-RAY DIFFRACTION	9	chain 'B' and (resid 57 through 68 )
10	X-RAY DIFFRACTION	10	chain 'B' and (resid 69 through 90 )
11	X-RAY DIFFRACTION	11	chain 'B' and (resid 91 through 98 )
12	X-RAY DIFFRACTION	12	chain 'B' and (resid 99 through 110 )
13	X-RAY DIFFRACTION	13	chain 'B' and (resid 111 through 116 )
14	X-RAY DIFFRACTION	14	chain 'C' and (resid 1 through 10 )
15	X-RAY DIFFRACTION	15	chain 'C' and (resid 11 through 24 )
16	X-RAY DIFFRACTION	16	chain 'C' and (resid 25 through 56 )
17	X-RAY DIFFRACTION	17	chain 'C' and (resid 57 through 79 )
18	X-RAY DIFFRACTION	18	chain 'C' and (resid 80 through 98 )
19	X-RAY DIFFRACTION	19	chain 'C' and (resid 99 through 110 )
20	X-RAY DIFFRACTION	20	chain 'C' and (resid 111 through 116 )
21	X-RAY DIFFRACTION	21	chain 'D' and (resid 1 through 10 )
22	X-RAY DIFFRACTION	22	chain 'D' and (resid 11 through 24 )
23	X-RAY DIFFRACTION	23	chain 'D' and (resid 25 through 35 )
24	X-RAY DIFFRACTION	24	chain 'D' and (resid 36 through 47 )
25	X-RAY DIFFRACTION	25	chain 'D' and (resid 48 through 56 )
26	X-RAY DIFFRACTION	26	chain 'D' and (resid 57 through 68 )
27	X-RAY DIFFRACTION	27	chain 'D' and (resid 69 through 79 )
28	X-RAY DIFFRACTION	28	chain 'D' and (resid 80 through 90 )
29	X-RAY DIFFRACTION	29	chain 'D' and (resid 91 through 98 )
30	X-RAY DIFFRACTION	30	chain 'D' and (resid 99 through 110 )
31	X-RAY DIFFRACTION	31	chain 'D' and (resid 111 through 116 )
32	X-RAY DIFFRACTION	32	chain 'E' and (resid 1 through 10 )
33	X-RAY DIFFRACTION	33	chain 'E' and (resid 11 through 24 )
34	X-RAY DIFFRACTION	34	chain 'E' and (resid 25 through 35 )
35	X-RAY DIFFRACTION	35	chain 'E' and (resid 36 through 56 )
36	X-RAY DIFFRACTION	36	chain 'E' and (resid 57 through 90 )
37	X-RAY DIFFRACTION	37	chain 'E' and (resid 91 through 110 )
38	X-RAY DIFFRACTION	38	chain 'E' and (resid 111 through 116 )
39	X-RAY DIFFRACTION	39	chain 'F' and (resid 1 through 10 )
40	X-RAY DIFFRACTION	40	chain 'F' and (resid 11 through 24 )
41	X-RAY DIFFRACTION	41	chain 'F' and (resid 25 through 35 )
42	X-RAY DIFFRACTION	42	chain 'F' and (resid 36 through 47 )
43	X-RAY DIFFRACTION	43	chain 'F' and (resid 48 through 68 )
44	X-RAY DIFFRACTION	44	chain 'F' and (resid 69 through 90 )
45	X-RAY DIFFRACTION	45	chain 'F' and (resid 91 through 104 )
46	X-RAY DIFFRACTION	46	chain 'F' and (resid 105 through 110 )
47	X-RAY DIFFRACTION	47	chain 'F' and (resid 111 through 115 )
48	X-RAY DIFFRACTION	48	chain 'G' and (resid 1 through 24 )
49	X-RAY DIFFRACTION	49	chain 'G' and (resid 25 through 67 )
50	X-RAY DIFFRACTION	50	chain 'G' and (resid 68 through 79 )
51	X-RAY DIFFRACTION	51	chain 'G' and (resid 80 through 116 )
52	X-RAY DIFFRACTION	52	chain 'H' and (resid 1 through 10 )
53	X-RAY DIFFRACTION	53	chain 'H' and (resid 11 through 24 )
54	X-RAY DIFFRACTION	54	chain 'H' and (resid 25 through 56 )
55	X-RAY DIFFRACTION	55	chain 'H' and (resid 57 through 79 )
56	X-RAY DIFFRACTION	56	chain 'H' and (resid 80 through 98 )
57	X-RAY DIFFRACTION	57	chain 'H' and (resid 99 through 116 )
58	X-RAY DIFFRACTION	58	chain 'I' and (resid 1 through 10 )
59	X-RAY DIFFRACTION	59	chain 'I' and (resid 11 through 24 )
60	X-RAY DIFFRACTION	60	chain 'I' and (resid 25 through 35 )
61	X-RAY DIFFRACTION	61	chain 'I' and (resid 36 through 56 )
62	X-RAY DIFFRACTION	62	chain 'I' and (resid 57 through 90 )
63	X-RAY DIFFRACTION	63	chain 'I' and (resid 91 through 98 )
64	X-RAY DIFFRACTION	64	chain 'I' and (resid 99 through 110 )
65	X-RAY DIFFRACTION	65	chain 'I' and (resid 111 through 116 )
66	X-RAY DIFFRACTION	66	chain 'J' and (resid 1 through 24 )
67	X-RAY DIFFRACTION	67	chain 'J' and (resid 25 through 56 )
68	X-RAY DIFFRACTION	68	chain 'J' and (resid 57 through 68 )
69	X-RAY DIFFRACTION	69	chain 'J' and (resid 69 through 79 )
70	X-RAY DIFFRACTION	70	chain 'J' and (resid 80 through 110 )
71	X-RAY DIFFRACTION	71	chain 'J' and (resid 111 through 116 )
72	X-RAY DIFFRACTION	72	chain 'K' and (resid 1 through 10 )
73	X-RAY DIFFRACTION	73	chain 'K' and (resid 11 through 24 )
74	X-RAY DIFFRACTION	74	chain 'K' and (resid 25 through 35 )
75	X-RAY DIFFRACTION	75	chain 'K' and (resid 36 through 68 )
76	X-RAY DIFFRACTION	76	chain 'K' and (resid 69 through 90 )
77	X-RAY DIFFRACTION	77	chain 'K' and (resid 91 through 116 )
78	X-RAY DIFFRACTION	78	chain 'L' and (resid 1 through 10 )
79	X-RAY DIFFRACTION	79	chain 'L' and (resid 11 through 47 )
80	X-RAY DIFFRACTION	80	chain 'L' and (resid 48 through 79 )
81	X-RAY DIFFRACTION	81	chain 'L' and (resid 80 through 110 )
82	X-RAY DIFFRACTION	82	chain 'L' and (resid 111 through 116 )
83	X-RAY DIFFRACTION	83	chain 'M' and (resid 273 through 278 )
84	X-RAY DIFFRACTION	84	chain 'N' and (resid 273 through 278 )
85	X-RAY DIFFRACTION	85	chain 'O' and (resid 273 through 279 )
86	X-RAY DIFFRACTION	86	chain 'P' and (resid 273 through 279 )
87	X-RAY DIFFRACTION	87	chain 'Q' and (resid 273 through 279 )
88	X-RAY DIFFRACTION	88	chain 'R' and (resid 273 through 278 )
89	X-RAY DIFFRACTION	89	chain 'S' and (resid 273 through 278 )
90	X-RAY DIFFRACTION	90	chain 'T' and (resid 273 through 279 )
91	X-RAY DIFFRACTION	91	chain 'U' and (resid 273 through 277 )
92	X-RAY DIFFRACTION	92	chain 'V' and (resid 273 through 278 )
93	X-RAY DIFFRACTION	93	chain 'X' and (resid 273 through 276 )