Mass: 18.015 Da / Num. of mol.: 222 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
Sequence details
The sequence data was deposited in the DDBJ under accession number LC639952. The gene was directly ...The sequence data was deposited in the DDBJ under accession number LC639952. The gene was directly obtained from the gut of honeybees, and residues I107V, P259A, V352A were considered to be naturally occurred.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.63 Å3/Da / Density % sol: 53.22 %
Resolution: 2→48.8 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.947 / SU B: 3.358 / SU ML: 0.093 / Cross valid method: THROUGHOUT / ESU R: 0.154 / ESU R Free: 0.139 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.21228
2091
4.9 %
RANDOM
Rwork
0.17923
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obs
0.18083
40529
99.98 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK