+Open data
-Basic information
Entry | Database: PDB / ID: 7v67 | ||||||
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Title | Crystal Structure of Enolase1 from Candida albicans | ||||||
Components | Enolase 1 | ||||||
Keywords | METAL BINDING PROTEIN / Enolase1 / Candida albicans | ||||||
Function / homology | Function and homology information fungal-type cell wall organization or biogenesis / high molecular weight kininogen binding / filamentous growth of a population of unicellular organisms in response to biotic stimulus / yeast-form cell wall / : / fungal biofilm matrix / hyphal cell wall / protein-glutamine gamma-glutamyltransferase activity / phosphopyruvate hydratase / phosphopyruvate hydratase complex ...fungal-type cell wall organization or biogenesis / high molecular weight kininogen binding / filamentous growth of a population of unicellular organisms in response to biotic stimulus / yeast-form cell wall / : / fungal biofilm matrix / hyphal cell wall / protein-glutamine gamma-glutamyltransferase activity / phosphopyruvate hydratase / phosphopyruvate hydratase complex / phosphopyruvate hydratase activity / fungal-type cell wall / symbiont entry into host / filamentous growth / fibrinolysis / gluconeogenesis / glycolytic process / extracellular vesicle / external side of plasma membrane / cell surface / magnesium ion binding / extracellular region / membrane / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Candida albicans SC5314 (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Zhang, M. / Zhang, X. | ||||||
Funding support | China, 1items
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Citation | Journal: Microbiol Spectr / Year: 2022 Title: Baicalein Acts against Candida albicans by Targeting Eno1 and Inhibiting Glycolysis. Authors: Li, L. / Lu, H. / Zhang, X. / Whiteway, M. / Wu, H. / Tan, S. / Zang, J. / Tian, S. / Zhen, C. / Meng, X. / Li, W. / Zhang, D. / Zhang, M. / Jiang, Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7v67.cif.gz | 644.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7v67.ent.gz | 534.9 KB | Display | PDB format |
PDBx/mmJSON format | 7v67.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7v67_validation.pdf.gz | 469 KB | Display | wwPDB validaton report |
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Full document | 7v67_full_validation.pdf.gz | 476.3 KB | Display | |
Data in XML | 7v67_validation.xml.gz | 59.8 KB | Display | |
Data in CIF | 7v67_validation.cif.gz | 84.1 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v6/7v67 ftp://data.pdbj.org/pub/pdb/validation_reports/v6/7v67 | HTTPS FTP |
-Related structure data
Related structure data | 7vrdC 2al2S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 48116.219 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida albicans SC5314 (yeast) / Strain: SC5314 / Gene: ENO1 / Production host: Escherichia coli (E. coli) / References: UniProt: P30575, phosphopyruvate hydratase #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.95 % Description: THE ENTRY CONTAINS FRIEDEL PAIRS IN I/F_PLUS/MINUS COLUMNS. |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 0.2 M Lithium sulfate monohydrate, 0.1 M BIS-TRIS pH 6.5, 25% w/v Polyethylene glycol 3,350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.9792 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jan 16, 2021 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2→33.114 Å / Num. obs: 116013 / % possible obs: 98.9 % / Redundancy: 5.5 % / Rmerge(I) obs: 0.034 / Rpim(I) all: 0.034 / Rrim(I) all: 0.049 / Net I/σ(I): 12.79 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 2AL2 Resolution: 2→33.114 Å / SU ML: 0.19 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 23.13 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 93.38 Å2 / Biso mean: 28.853 Å2 / Biso min: 12.92 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2→33.114 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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