+Open data
-Basic information
Entry | Database: PDB / ID: 7v2w | ||||||
---|---|---|---|---|---|---|---|
Title | protomer structure from the dimer of yeast THO complex | ||||||
Components |
| ||||||
Keywords | TRANSCRIPTION / RNA transcription | ||||||
Function / homology | Function and homology information nucleoplasmic THO complex / cellular response to azide / THO complex / THO complex part of transcription export complex / positive regulation of transcription elongation by RNA polymerase I / transcription export complex / Cdc73/Paf1 complex / mRNA 3'-end processing / positive regulation of transcription by RNA polymerase I / transcription-coupled nucleotide-excision repair ...nucleoplasmic THO complex / cellular response to azide / THO complex / THO complex part of transcription export complex / positive regulation of transcription elongation by RNA polymerase I / transcription export complex / Cdc73/Paf1 complex / mRNA 3'-end processing / positive regulation of transcription by RNA polymerase I / transcription-coupled nucleotide-excision repair / mRNA export from nucleus / stress granule assembly / transcription elongation by RNA polymerase II / mRNA processing / DNA recombination / nucleic acid binding / chromosome, telomeric region / molecular adaptor activity / mRNA binding / nucleus Similarity search - Function | ||||||
Biological species | Saccharomyces cerevisiae S288c (yeast) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.2 Å | ||||||
Authors | Chen, C. / Tan, M. / Wu, Z. / Wu, J. / Lei, M. | ||||||
Funding support | China, 1items
| ||||||
Citation | Journal: Sci Bull (Beijing) / Year: 2021 Title: Structural and functional insights into R-loop prevention and mRNA export by budding yeast THO-Sub2 complex. Authors: Cong Chen / Ming Tan / Zhenfang Wu / Yuebin Zhang / Fanyang He / Yanjia Lu / Shaobai Li / Mi Cao / Guohui Li / Jian Wu / Hong Cheng / Ming Lei / | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 7v2w.cif.gz | 497.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb7v2w.ent.gz | 394.1 KB | Display | PDB format |
PDBx/mmJSON format | 7v2w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 7v2w_validation.pdf.gz | 871.9 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 7v2w_full_validation.pdf.gz | 883.3 KB | Display | |
Data in XML | 7v2w_validation.xml.gz | 66.3 KB | Display | |
Data in CIF | 7v2w_validation.cif.gz | 104.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v2/7v2w ftp://data.pdbj.org/pub/pdb/validation_reports/v2/7v2w | HTTPS FTP |
-Related structure data
Related structure data | 31669MC 7v2yC C: citing same article (ref.) M: map data used to model this data |
---|---|
Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
|
---|---|
1 |
|
-Components
#1: Protein | Mass: 87938.547 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: S288c / Gene: HPR1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P17629 |
---|---|
#2: Protein | Mass: 184163.734 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: S288c / Gene: THO2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P53552 |
#3: Protein | Mass: 47229.652 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: S288c / Gene: TEX1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P53851 |
#4: Protein | Mass: 45055.012 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: S288c / Gene: MFT1 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P33441 |
#5: Protein | Mass: 30340.264 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Saccharomyces cerevisiae S288c (yeast) / Strain: S288c / Gene: THP2 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: O13539 |
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: THO Complex / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
---|---|
Source (natural) | Organism: Saccharomyces cerevisiae S288c (yeast) / Strain: S288c |
Source (recombinant) | Organism: Spodoptera frugiperda (fall armyworm) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
---|---|
3D reconstruction | Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 393530 / Symmetry type: POINT |