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Open data
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Basic information
Entry | Database: PDB / ID: 7v1q | ||||||
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Title | Leifsonia Alcohol Dehydrogenases LnADH | ||||||
![]() | Alcohol Dehydrogenases | ||||||
![]() | BIOSYNTHETIC PROTEIN / Alcohol / Dehydrogenases | ||||||
Function / homology | ISOPROPYL ALCOHOL / NICOTINAMIDE-ADENINE-DINUCLEOTIDE![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Song, Y. / Qu, X. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Engineering Leifsonia Alcohol Dehydrogenase for Thermostability and Catalytic Efficiency by Enhancing Subunit Interactions. Authors: Zhu, L. / Song, Y. / Chang, C. / Ma, H. / Yang, L. / Deng, Z. / Deng, W. / Qu, X. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 704.7 KB | Display | ![]() |
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PDB format | ![]() | 605.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.6 MB | Display | ![]() |
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Full document | ![]() | 2.6 MB | Display | |
Data in XML | ![]() | 75.7 KB | Display | |
Data in CIF | ![]() | 107.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 25260.756 Da / Num. of mol.: 8 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-IPA / #3: Chemical | ChemComp-NAD / #4: Chemical | ChemComp-ZN / #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.75 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.1M Sodium acetate/Acetic acid, pH 7.0, 0.2M Zinc acetate, 20% PEG 1000 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jan 9, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 1.58→50 Å / Num. obs: 219460 / % possible obs: 100 % / Redundancy: 6.8 % / Rmerge(I) obs: 0.149 / Net I/σ(I): 15.7 |
Reflection shell | Resolution: 1.58→1.61 Å / Rmerge(I) obs: 0.559 / Mean I/σ(I) obs: 3.1 / Num. unique obs: 11079 |
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Processing
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Refinement | Method to determine structure: ![]() Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 54.67 Å2 / Biso mean: 10.53 Å2 / Biso min: 1.26 Å2
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Refinement step | Cycle: final / Resolution: 1.58→49.56 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.581→1.622 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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