+Open data
-Basic information
Entry | Database: PDB / ID: 7uv4 | ||||||
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Title | Pis v 3.0101 vicilin leader sequence residues 56-115 | ||||||
Components | Vicilin Pis v 3.0101 | ||||||
Keywords | ALLERGEN / Seed storage | ||||||
Function / homology | Cupin / Cupin 1 / Cupin / nutrient reservoir activity / RmlC-like cupin domain superfamily / RmlC-like jelly roll fold / Vicilin Pis v 3.0101 Function and homology information | ||||||
Biological species | Pistacia vera (pistachio) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Mueller, G.A. / Foo, A.C.Y. / DeRose, E.F. | ||||||
Funding support | United States, 1items
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Citation | Journal: J.Agric.Food Chem. / Year: 2023 Title: Structure and IgE Cross-Reactivity among Cashew, Pistachio, Walnut, and Peanut Vicilin-Buried Peptides. Authors: Foo, A.C.Y. / Nesbit, J.B. / Gipson, S.A.Y. / DeRose, E.F. / Cheng, H. / Hurlburt, B.K. / Kulis, M.D. / Kim, E.H. / Dreskin, S.C. / Mustafa, S. / Maleki, S.J. / Mueller, G.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7uv4.cif.gz | 381.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7uv4.ent.gz | 323.2 KB | Display | PDB format |
PDBx/mmJSON format | 7uv4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uv/7uv4 ftp://data.pdbj.org/pub/pdb/validation_reports/uv/7uv4 | HTTPS FTP |
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-Related structure data
Related structure data | 7uv1C 7uv2C 7uv3C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 7309.122 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pistacia vera (pistachio) / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B4X640 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution / Contents: 50 uM DSS, 90% H2O/10% D2O / Details: 90% PBS pH 7.4, 50 uM DSS reference standard / Label: 13C_15N / Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 50 uM / Component: DSS / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength: 150 mM / Label: PBS / pH: 7.5 / Pressure: 1 atm / Temperature: 298.15 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 6 | |||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||||||||
NMR ensemble | Conformer selection criteria: all calculated structures submitted Conformers calculated total number: 20 / Conformers submitted total number: 20 |