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- PDB-7uqw: PCC6803 Cyanophycinase S132DAP covalently bound to cyanophycin dimer -
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Open data
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Basic information
Entry | Database: PDB / ID: 7uqw | ||||||
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Title | PCC6803 Cyanophycinase S132DAP covalently bound to cyanophycin dimer | ||||||
![]() | Cyanophycinase | ||||||
![]() | HYDROLASE / cyanophycinase / CphB / cyanophycin | ||||||
Function / homology | ![]() cyanophycinase / serine-type peptidase activity / protein homodimerization activity / proteolysis Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Sharon, I. / Schmeing, T.M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The structure of cyanophycinase in complex with a cyanophycin degradation intermediate. Authors: Sharon, I. / Grogg, M. / Hilvert, D. / Schmeing, T.M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 179.1 KB | Display | ![]() |
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PDB format | ![]() | 138 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 2.1 MB | Display | ![]() |
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Full document | ![]() | 2.1 MB | Display | |
Data in XML | ![]() | 35.8 KB | Display | |
Data in CIF | ![]() | 51.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7uqvC ![]() 3en0S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30117.398 Da / Num. of mol.: 3 Mutation: S132DAP (diaminopropanoate: a modified amino acid) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-7ID / ( #3: Chemical | ChemComp-SO4 / | #4: Chemical | ChemComp-ARF / #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.4 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, sitting drop Details: 0.1 M MES pH 6.6, 1.65 M ammonium sulfate and 4% formamide |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Apr 13, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97918 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→61.61 Å / Num. obs: 133142 / % possible obs: 99.92 % / Redundancy: 12.9 % / Biso Wilson estimate: 19.6 Å2 / CC1/2: 0.997 / Net I/σ(I): 15.18 |
Reflection shell | Resolution: 1.5→1.53 Å / Mean I/σ(I) obs: 3.1 / Num. unique obs: 13155 / CC1/2: 0.866 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3EN0 Resolution: 1.5→61.61 Å / SU ML: 0.1446 / Cross valid method: FREE R-VALUE / σ(F): 1.89 / Phase error: 17.4545 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.53 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.5→61.61 Å
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Refine LS restraints |
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LS refinement shell |
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