温度: 290 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 8.5 詳細: Protein at 36 mg/mL was combined with 5 mM 2-PG and mixed 1:1 (0.2 uL protein and 0.2 uL precipitant) with 85 mM Tris/HCl pH 8.5, 25.5% (w/v) PEG4000, 15% glycerol, and 170 mM sodium acetate ...詳細: Protein at 36 mg/mL was combined with 5 mM 2-PG and mixed 1:1 (0.2 uL protein and 0.2 uL precipitant) with 85 mM Tris/HCl pH 8.5, 25.5% (w/v) PEG4000, 15% glycerol, and 170 mM sodium acetate (JCSG TOP96 A11). Cryo: Direct. Puck: vfn4-7