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- PDB-7ucw: Structure of mouse Decr1 in complex with 2'-5' oligoadenylate -

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Basic information

Entry
Database: PDB / ID: 7ucw
TitleStructure of mouse Decr1 in complex with 2'-5' oligoadenylate
ComponentsDecr1 protein
KeywordsOXIDOREDUCTASE / immunity
Function / homology
Function and homology information


2,4-dienoyl-CoA reductase (NADPH) activity / fatty acid beta-oxidation / catalytic complex / NADPH binding / mitochondrion / nucleoplasm / identical protein binding / cytosol
Similarity search - Function
Enoyl-(Acyl carrier protein) reductase / Short-chain dehydrogenase/reductase SDR / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
Chem-25L / Decr1 protein
Similarity search - Component
Biological speciesMus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.35 Å
AuthorsGovande, A.A. / Kranzusch, P.J.
Funding support United States, 1items
OrganizationGrant numberCountry
Richard and Susan Smith Family Foundation United States
CitationJournal: J.Gen.Virol. / Year: 2023
Title: RNase L-activating 2'-5' oligoadenylates bind ABCF1, ABCF3 and Decr-1.
Authors: Govande, A.A. / Babnis, A.W. / Urban, C. / Habjan, M. / Hartmann, R. / Kranzusch, P.J. / Pichlmair, A.
History
DepositionMar 17, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 22, 2023Provider: repository / Type: Initial release
Revision 1.1Sep 13, 2023Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / reflns
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _reflns.percent_possible_obs
Revision 1.2Oct 25, 2023Group: Refinement description / Category: pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Decr1 protein
B: Decr1 protein
C: Decr1 protein
D: Decr1 protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)134,6208
Polymers129,9574
Non-polymers4,6624
Water17,186954
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area19850 Å2
ΔGint-111 kcal/mol
Surface area37620 Å2
MethodPISA
Unit cell
Length a, b, c (Å)82.379, 117.191, 232.248
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Components on special symmetry positions
IDModelComponents
11D-725-

HOH

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Components

#1: Protein
Decr1 protein


Mass: 32489.334 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Decr1 / Production host: Escherichia coli (E. coli) / References: UniProt: Q4FJK0
#2: Chemical
ChemComp-25L / [[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-4-[[(2R,3R,4R,5R)-5-(6-aminopurin-9-yl)-4-[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-3-hydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl] phosphono hydrogen phosphate / 2'-5'-oligoadenylate trimer


Mass: 1165.593 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C30H40N15O25P5 / Feature type: SUBJECT OF INVESTIGATION
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 954 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.16 Å3/Da / Density % sol: 42.97 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop / Details: 34% PEG-300, 100 mM Tris-HCl pH 8.6

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Data collection

DiffractionMean temperature: 80 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 16, 2018
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 1.35→67.39 Å / Num. obs: 244100 / % possible obs: 99.8 % / Redundancy: 6.8 % / Biso Wilson estimate: 17.7 Å2 / CC1/2: 0.998 / Rpim(I) all: 0.031 / Net I/σ(I): 10.6
Reflection shellResolution: 1.35→1.37 Å / Mean I/σ(I) obs: 1.2 / Num. unique obs: 11679 / CC1/2: 0.525 / Rpim(I) all: 0.559

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
PDB_EXTRACT3.27data extraction
XDSdata reduction
Aimlessdata scaling
AutoSolphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1W6U
Resolution: 1.35→67.39 Å / SU ML: 0.1344 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 16.4363
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.1598 2000 0.82 %
Rwork0.1512 241889 -
obs0.1512 243889 99.74 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 25.27 Å2
Refinement stepCycle: LAST / Resolution: 1.35→67.39 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8465 0 300 954 9719
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00838987
X-RAY DIFFRACTIONf_angle_d1.084312228
X-RAY DIFFRACTIONf_chiral_restr0.07781385
X-RAY DIFFRACTIONf_plane_restr0.00981515
X-RAY DIFFRACTIONf_dihedral_angle_d14.50993207
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.35-1.380.28131380.270516746X-RAY DIFFRACTION97.84
1.38-1.420.22581430.242917149X-RAY DIFFRACTION99.58
1.42-1.460.21861420.222417179X-RAY DIFFRACTION99.84
1.46-1.510.21951410.204817131X-RAY DIFFRACTION99.88
1.51-1.560.22631430.202117249X-RAY DIFFRACTION99.91
1.56-1.630.17981430.174317266X-RAY DIFFRACTION99.91
1.63-1.70.15941420.156917228X-RAY DIFFRACTION99.91
1.7-1.790.16951430.148517255X-RAY DIFFRACTION99.92
1.79-1.90.15851420.151817239X-RAY DIFFRACTION99.97
1.9-2.050.13331430.134817355X-RAY DIFFRACTION99.98
2.05-2.260.14131430.127217313X-RAY DIFFRACTION99.93
2.26-2.580.12681440.126417401X-RAY DIFFRACTION99.89
2.58-3.250.1511460.143717502X-RAY DIFFRACTION99.97
3.25-67.390.16551470.151717876X-RAY DIFFRACTION99.82
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
11.03492149238-0.265512646857-0.1814392904010.662706674965-0.1991085271040.1368103981480.0474667833264-0.02061459603660.1306421780490.05674207512-0.102368582403-0.22796730725-0.1653520491910.2559990871390.03002387421420.166054190447-0.0277454041564-0.009162585207580.2103658795170.01026932291890.228175883150.92222038146-4.969404381425.6863815147
20.32937817338-0.0651747343137-0.0316860490870.198744783110.001659903293760.0007823963569010.00139128935169-0.01126987039060.008104636096530.0141711139025-0.0178946677382-0.0722852075805-0.05457584501970.0318440932849-0.07151293685930.156670194473-0.01083854847560.0123271486440.1631122518970.01402919414590.166093776222-12.72998382813.9960677557618.8696023658
30.734004736552-0.803300629222-0.2306635499520.8222005419570.273322812931.097078572170.05922029146630.04117657298750.226000168475-0.0599067945680.008962009099330.0227000372816-0.201235853225-0.0483819005957-0.1926298890810.18231856941-0.00488870447770.01959088258940.1290136114070.01364305500820.179359088297-20.297068306810.54141052717.6563181589
4-0.05444458718420.146834481248-0.1658235051490.41045973793-0.3373140704340.1752316285150.050545435143-0.01015909018270.06392222996190.110310662315-0.0412696575370.00535229816485-0.2873952811680.0358181263592-0.1355427890640.1976451786330.004304900615840.01979048443570.1724465822120.0007490004278810.146954670427-23.9623758319-1.4020216083629.6629971729
50.3757054423390.1146602620960.2691087086250.675512760682-0.2663211696290.340469242341-0.04914107902530.01002676844760.08372517035930.02299530478220.02955303748940.162185729927-0.248062623664-0.315276742657-0.13150291260.1780415666660.02525177702110.01858047450570.1725459551130.01498903177940.157518171534-30.7668994876-1.7462069412721.7512592246
60.271860857360.288460429553-0.1250244359540.4975856293620.05678963776550.200804382654-0.00119397569204-0.03233449620180.0470519617716-0.0328908164222-0.00137645194023-0.0114501372514-0.1220164754150.0885329848772-0.04593700107370.1484337482390.01675201618930.0109497656010.1606645630370.005315358116780.130892178555-20.7316080579-8.9597882198425.5467411624
70.190120706888-0.1492009787460.03545284033430.2390443354050.02627185739780.2217654922060.0566543919917-0.03945108109330.037216263943-0.04193982966370.03189730289920.0354488153839-0.0546521058818-0.02592735541880.07777546148050.153916634488-0.0006386129989950.008709951233580.1547037159630.003954048462040.13183977713-26.2428412147-11.409739898824.5970255698
80.521015676703-0.1513676044650.1435274772610.436389445313-0.3299772647230.598407033926-0.018185825045-0.06841262111390.04358238940620.007194581833570.00251756999858-0.0342929302301-0.0264773738766-0.005993366461180.08226910864240.1430633163630.006509586192180.01274339494660.1713455244130.003519727134550.146144161364-11.4821856842-10.83491122926.1088423216
90.4573907153240.158453311475-0.0317764684130.741526823429-0.1102568649940.0273822132037-0.133994657363-0.3146554789070.204952818150.5137463985130.080818013126-0.1556549945260.09480235137310.4195764298020.0126970453780.3550481168760.0781044447717-0.06486787862290.464692870247-0.00474377908340.2139088988881.36582720843-27.433046061349.1076069616
100.833899446852-0.1413911965130.1219801102110.7788722972120.5100535806720.646072829495-0.00433679879892-0.180015883455-0.1979002416780.3185327026920.06844407143620.007030970866850.1602519332260.005230388685770.03689554615540.2493044644760.007616377775990.05062004093090.2003308569460.05454029800030.176771702416-32.3318370195-35.727973518350.52383711
110.07580976012910.0512474051638-0.1202846577560.21907684692-0.0491480098280.191443553389-0.0399923105329-0.0266392448508-0.1532210343070.01807686666750.008913407264690.082439826350.156717785842-0.0528473345080.03476704085670.2435439005410.01649353478510.04451034870790.1692673021570.04397662540950.230718908777-25.8492477993-49.520935964941.9123962207
120.1868135181750.06745088608940.0360792579850.1644361395910.1242043522570.128690046586-0.174838979971-0.305384499891-0.6095219693360.1075685740670.06287349008960.0328855574870.2610805829250.05739592789152.86867524023E-50.3164183210640.05982225616740.07297504312490.2348517083340.1238086487070.322972516461-22.5894802351-56.120395108545.4003997467
130.161704502655-0.0624784967528-0.01376341218310.4069490032870.1650225399470.242775655519-0.0501442001432-0.0809153480863-0.3287301570080.03200907295170.02888106278810.04631138517030.3033877080970.159063820040.1104774416230.3435764246040.06141415533250.05235593603360.1826894557670.04512723411670.344450566242-17.7382060975-58.217132046233.8307236693
140.357542711250.211045601076-0.1114442701960.471495948624-0.1447152603410.3800113210930.0563817203621-0.0995862165774-0.2323521532880.13289141858-0.0641166455937-0.0710644030950.1557435331720.0848542598928-0.2928317364040.2015192642690.04425541650120.009981891581490.1885773678330.03367515177910.213131406946-11.8700603054-42.189295304137.1392989699
150.106177035715-0.102016073361-0.07318335623310.3511773176260.05628819464070.269978356235-0.03115099515120.1683935182-0.369410652958-0.1105577562290.0731536213168-0.2573764854960.2073030597650.202174151829-0.0909128204150.2521645490650.04519838239550.02631189116740.1740614731490.01315441213340.253568739772-12.7687478441-47.373569141428.1061212661
160.32695419942-0.199331508733-0.07717452241830.284745383266-0.1783355552660.3000958869580.00109183888347-0.0643329516126-0.07274859816470.0393889914819-0.01829476821960.02656707356640.1379174410620.06652806107750.006757619316170.1663720690370.02601254745770.01749584480450.1666712145350.009618394338810.163090522179-18.599857021-37.205270057633.4792946192
170.170399859196-0.0449530405103-0.09234872598620.09493052962770.04578186019170.1760051361120.0181951174869-0.0731944551428-0.19259783792-0.0264995948725-0.00332193044198-0.04634991841890.1446479850570.0694923211795-0.06453985346720.177163416040.02362330571520.01384262485030.1631238919890.0165187494550.166034179889-15.9766569738-36.767726335727.9271699792
180.261283149914-0.0100727707793-0.04690946203180.0636678283442-0.01216771512870.0154924653739-0.03916409765130.0255597469949-0.0544867491257-0.04243981658230.0583639091404-0.02435406702750.09178717185450.105069507883-0.07915380269780.2072110524860.00503690867110.03390121377530.149469550036-0.002934735379230.178810830612-26.1401841698-41.413567024626.0200194401
190.121706462114-0.01451526645640.0460209738290.0539814779350.04971395656640.0731197159945-0.0237670737196-0.8098937279420.006657860582440.329343535433-0.136122011457-0.527277731923-0.01286507420030.5796809084640.000481920282670.39206667205-0.0068383615204-0.04292423233730.5770335855680.02680301186740.396262253208-14.4979399244-28.461911525152.2542423707
200.40583738307-0.01494569414770.05394235170690.472186969435-0.2330059123420.453212196696-0.0178350764742-0.0872988586259-0.1350261471430.1373214652980.05567400468180.06735018832490.1917029094430.03883772347780.1608447653960.2003483054390.02046976238860.02658752147640.1893184220430.03348631403380.166346464237-27.3114072395-35.395415282345.9943479224
210.1966446504430.0782762172529-0.09526191611690.239880444419-0.1134357733580.260286357363-0.0638092857422-0.00962637346211-0.0605001255475-0.02528313499310.02420491992140.06516370628490.05009473080080.034381481406-0.007883492181320.1602669911530.007372050767530.01944719266690.1679479384440.01085878468050.168730836414-26.3653611874-29.543362586836.5636095292
220.18855323362-0.120236511707-0.1507751587750.4927346230840.03525829451710.144854372208-0.0963488300786-0.4832086684730.2368059895840.4089768073220.0522773539788-0.359746010902-0.1820796812010.0754203805428-0.005382715044170.3380744803650.00780975886825-0.04242528456520.362095854229-0.09561647599990.21134772124-21.2043816368-4.8396584536952.0360786163
230.5442749278330.0522217268820.1917178188921.13900856273-0.09332237180970.7214000412670.03649651308460.2436028170060.087054634542-0.257615365896-0.043932189616-0.1879184181530.04089585559350.1164717684850.0881590255130.2064090407510.01825234916890.05443763546640.2277603082850.03421431694490.156681520005-6.91108334424-12.63915092435.66142988792
240.0309173506489-0.05766469205810.02764465741930.215518541214-0.2218453496670.253023831161-0.0067619263602-0.01959162889270.0413465065883-0.158063458202-0.0560258991888-0.1795723300130.051020330440.128327838360.01949245001130.1969293120020.04711229628320.06763988926260.2353341041730.0420185470490.2416449714755.6623206232-22.584050463113.2592623259
250.40205068197-0.4175152082270.01031278547130.6845135219050.2936876953090.407695863695-0.006725961904730.01469990211730.000189264036081-0.297014274209-0.149776952384-0.5212258103430.2446761429360.2767542426730.07544583699060.2539358389180.09636278151590.1303566195820.2810725105980.06248918047890.29701151058512.0908266399-30.004016944715.2937181032
260.3739590568720.0725562512597-0.2518954991490.16870285437-0.112355904970.359848404291-0.08828249741780.185062271568-0.133391582322-0.1840607096350.0773630201318-0.2370495235470.118648783870.163101966961-0.2643228910730.2102870532330.0494688242610.04292766031420.2034762334210.003368854009790.220559329926-4.13642416915-35.003862917317.7680575365
270.557108862342-0.316311943546-0.06391418967840.288932990906-0.03474043298880.3135942722110.09596328844150.0123451201021-0.4340877137620.132286534667-0.00913409382252-0.4016383869230.2261201055630.187062432104-0.3584136342690.1812845304220.05654669972970.03386684135670.2199545186230.01057651346690.2410656320171.39761482402-35.879056107826.5968476063
280.1524860972960.0174280755288-0.1499676324640.488619689858-0.1716388399970.1916160857-0.06795208256910.02882845160280.0402536389691-0.132270471993-0.0018362314084-0.03985409506820.08140619409660.117311928953-0.03092380894630.1736359966960.0304976973150.02284129370450.1828447611840.007163128940730.174627950232-7.47702433875-27.599128439521.9912385201
290.1365371407850.00693128781664-0.1198386419220.166717936071-0.02522242576060.11972884127-0.028862879026-0.0228193144703-0.124596942117-0.05596754145370.0311048096328-0.188811832070.1155943902470.121302352105-0.0611194415050.1752268152470.02866683303360.02212711452670.1837610217510.006178887494580.160383028536-8.18111493017-30.376519629127.4199459288
300.717237418223-0.262548536978-0.07282740671391.17695322247-0.7114793008560.501838270920.06312999272040.112835811464-0.18781521183-0.2822732930920.03065979405360.04637520994320.2606901739940.02341701612860.01136669372810.1964808481410.01717776851620.01543086915850.185744859549-0.001082262787320.168045061472-8.87318490028-24.359677303317.0535392729
310.198054598061-0.08310178059740.01885393095380.292471026010.01079667199490.1986324458190.04492674059890.12046359613-0.0467416232454-0.1089616473-0.0056294584426-0.1360741099130.131549065640.184460250306-0.01213084786690.1787569964870.02089496509870.02942462802010.1932442881280.006369265472940.157147067993-8.01621884988-17.875977874410.0168637912
320.1011803252520.0155326057222-0.08121039500010.324424899322-0.1114002159890.08163894454120.00254969483438-0.0170601027364-0.0230779704066-0.0501726284678-0.00703974414525-0.07179613477330.04240434802480.0325977084572-0.0006096016426920.15208403870.01007274190250.01686383865710.1726343911180.007268062109980.15827190902-10.8122354072-17.515564385920.1161258805
330.669005774751-0.272646546395-0.01360785745680.25619764631-0.2790085383370.686698697153-0.04389478708210.191197645232-0.179590208902-0.592275015904-0.2868160918250.4616246505860.210731190754-0.238169273441-0.1794904696890.3249804187750.013973348732-0.1696313575150.265850983804-0.07170572633810.272142181248-37.9235071401-18.56349500337.0527285747
340.3555217373430.0675256844592-0.2600194828670.975367381479-0.2406965339090.55199398111-0.04888632639280.11352155215-0.09379293429850.08014973856540.03742779557350.2885281676350.249033119358-0.3856029195760.07272493481670.210408769054-0.03753873139160.03932901955760.2050958932080.005617302945430.25959675764-42.5145495365-40.655538108731.2488054365
350.09175369082310.00155834290521-0.03881905129780.394763207840.1172318349020.0560191476712-0.0392013081104-0.0479715867188-0.06719801965950.123336350186-0.007689369811260.352448395710.11124456384-0.127386314388-0.09843339789750.161566891366-0.02096968102050.04722191163710.2029730272650.01158821103660.257112973469-48.186391469-25.757935156138.648160133
360.229496472651-0.0679741087123-0.1171464826650.2901623545770.1322744832160.43399387663-0.05514828392790.0720571589283-0.1535462053060.1509321000190.1028907675470.6477873626430.0338376914765-0.277837473208-0.188941336750.129445197072-0.0204247170670.04661353312620.2751571614270.02200099061370.422924916111-55.7546987867-22.838356480238.2915024348
370.390038658089-0.0112484838454-0.2611332052340.6867041257130.07686886936690.354676362136-0.03290573491850.03317807354930.02276347663070.0838269909240.03226428540960.270638462501-0.00794801795721-0.192859341765-0.2974159553740.1297661955870.01502402688290.03358606059020.2001041387370.01927268975710.244167766154-46.2376741866-13.46649509336.244679569
380.210315525650.0287807532732-0.2802081362890.5270269103010.06215916285660.4025008974450.04937570599210.04592379029310.09171956446620.142816501764-0.01061049020590.178136630847-0.342639094097-0.222463169042-0.2390461807450.1519388895090.02824572260230.04298522862010.1999513445760.02527256226770.182229543144-38.8481034354-9.0696203781336.4699961528
390.1068483934020.0612704794447-0.1265119000460.473705969018-0.1463225257040.151416062169-0.0292415696867-0.02144969535910.0124065119437-0.01346258128390.004363261852630.1057958823950.105338764514-0.0561407085575-0.06279179286020.1456617313160.003574434343270.009066445583520.1766118966870.01165871169750.152920966686-34.1153235802-20.244437069931.7155280988
400.09587873364360.006482357608030.09055585427670.273975622899-0.06151555830490.2688956162610.0183628448559-0.0790177872424-0.01827554253690.03146710488660.07971717063240.1019906214-0.0312162450535-0.1347172566910.01989004828180.1471790436550.01060319643270.008603348813530.1752405730220.01357341317150.144689567528-30.5460159504-15.296920380732.8249278348
410.8390424476750.226854389834-0.1010794739321.5001785457-0.3430443268520.784799718238-0.07818088574720.156791593663-0.063084828109-0.2914427819550.05826732483130.2464312022620.107027296629-0.06945190908450.2537424321640.163535479198-0.00797127645519-0.005083836201210.1714782760680.01212413569120.17326029919-36.0882347504-25.526970123228.8467814885
420.3563831203030.0746139644414-0.0896076727160.2305582159060.02564868629790.165609381949-0.0501476928063-0.0284989671085-0.0130839810231-0.00833261738908-0.04641551278410.1930871991320.0408167561847-0.165085577292-0.01193177178090.166460488718-0.01495227840760.004857471661780.1714586241220.002536807031620.196801453401-39.9708471614-34.265481876129.7400081435
430.0776911853094-0.0303237641969-0.1069717572260.439305363026-0.02396365704820.134293177849-0.0378431240525-0.028670783897-0.03212234292960.0266389912910.009858361158430.07650933585510.023815822354-0.0177300159767-0.05627746667440.159235583221-0.003239866232380.02281874665470.1706111141430.01365107938210.162866307449-29.5720609024-30.51208975330.9761208486
441.124944020630.4647954726940.05276164038850.9904235206610.4199360346680.2077819929120.03950195381710.4747278605910.0576861479063-0.488535115508-0.04119791340050.222819304370.05572556096880.02535990209840.04890424398390.4526016058780.0126221125485-0.009536402950180.261197897531-0.05692949481520.14861357184-22.0153157925-43.93077576455.98255739807
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 35 through 58 )AA35 - 581 - 24
22chain 'A' and (resid 59 through 81 )AA59 - 8125 - 47
33chain 'A' and (resid 82 through 134 )AA82 - 13448 - 100
44chain 'A' and (resid 135 through 157 )AA135 - 157101 - 123
55chain 'A' and (resid 158 through 186 )AA158 - 186124 - 152
66chain 'A' and (resid 187 through 207 )AA187 - 207153 - 173
77chain 'A' and (resid 208 through 227 )AA208 - 227174 - 193
88chain 'A' and (resid 228 through 311 )AA228 - 311194 - 263
99chain 'A' and (resid 312 through 326 )AA312 - 326264 - 278
1010chain 'B' and (resid 35 through 58 )BB35 - 581 - 24
1111chain 'B' and (resid 59 through 81 )BB59 - 8125 - 47
1212chain 'B' and (resid 82 through 107 )BB82 - 10748 - 73
1313chain 'B' and (resid 108 through 134 )BB108 - 13474 - 100
1414chain 'B' and (resid 135 through 157 )BB135 - 157101 - 123
1515chain 'B' and (resid 158 through 186 )BB158 - 186124 - 152
1616chain 'B' and (resid 187 through 207 )BB187 - 207153 - 173
1717chain 'B' and (resid 208 through 227 )BB208 - 227174 - 193
1818chain 'B' and (resid 228 through 240 )BB228 - 240194 - 206
1919chain 'B' and (resid 241 through 265 )BB241 - 265207 - 220
2020chain 'B' and (resid 266 through 284 )BB266 - 284221 - 239
2121chain 'B' and (resid 285 through 311 )BB285 - 311240 - 266
2222chain 'B' and (resid 312 through 326 )BB312 - 326267 - 281
2323chain 'C' and (resid 35 through 58 )CC35 - 581 - 24
2424chain 'C' and (resid 59 through 81 )CC59 - 8125 - 47
2525chain 'C' and (resid 82 through 134 )CC82 - 13448 - 100
2626chain 'C' and (resid 135 through 157 )CC135 - 157101 - 123
2727chain 'C' and (resid 158 through 186 )CC158 - 186124 - 152
2828chain 'C' and (resid 187 through 207 )CC187 - 207153 - 173
2929chain 'C' and (resid 208 through 227 )CC208 - 227174 - 193
3030chain 'C' and (resid 228 through 265 )CC228 - 265194 - 219
3131chain 'C' and (resid 266 through 284 )CC266 - 284220 - 238
3232chain 'C' and (resid 285 through 308 )CC285 - 308239 - 262
3333chain 'C' and (resid 309 through 325 )CC309 - 325263 - 279
3434chain 'D' and (resid 36 through 58 )DD36 - 581 - 23
3535chain 'D' and (resid 59 through 81 )DD59 - 8124 - 46
3636chain 'D' and (resid 82 through 107 )DD82 - 10747 - 72
3737chain 'D' and (resid 108 through 157 )DD108 - 15773 - 122
3838chain 'D' and (resid 158 through 186 )DD158 - 186123 - 151
3939chain 'D' and (resid 187 through 207 )DD187 - 207152 - 172
4040chain 'D' and (resid 208 through 227 )DD208 - 227173 - 192
4141chain 'D' and (resid 228 through 265 )DD228 - 265193 - 219
4242chain 'D' and (resid 266 through 284 )DD266 - 284220 - 238
4343chain 'D' and (resid 285 through 311 )DD285 - 311239 - 265
4444chain 'D' and (resid 312 through 325 )DD312 - 325266 - 279

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