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Yorodumi- PDB-7u6p: Structure of an intellectual disability-associated ornithine deca... -
+Open data
-Basic information
Entry | Database: PDB / ID: 7u6p | ||||||||||||
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Title | Structure of an intellectual disability-associated ornithine decarboxylase variant G84R | ||||||||||||
Components | Ornithine decarboxylase | ||||||||||||
Keywords | HYDROLASE / ornithine decarboxylase / intellectual disability-associated variant | ||||||||||||
Function / homology | Function and homology information putrescine biosynthetic process from ornithine / ornithine decarboxylase / ornithine decarboxylase activity / Metabolism of polyamines / polyamine metabolic process / Regulation of ornithine decarboxylase (ODC) / regulation of protein catabolic process / kidney development / response to virus / cell population proliferation ...putrescine biosynthetic process from ornithine / ornithine decarboxylase / ornithine decarboxylase activity / Metabolism of polyamines / polyamine metabolic process / Regulation of ornithine decarboxylase (ODC) / regulation of protein catabolic process / kidney development / response to virus / cell population proliferation / positive regulation of cell population proliferation / protein homodimerization activity / cytosol / cytoplasm Similarity search - Function | ||||||||||||
Biological species | Homo sapiens (human) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | ||||||||||||
Authors | Zhou, X.E. / Schultz, C.R. / Powell, K.S. / Henrickson, A. / Lamp, J. / Brunzelle, J.S. / Demeler, B. / Vega, I.E. / Bachmann, A.S. / Melcher, K. | ||||||||||||
Funding support | United States, Canada, 3items
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Citation | Journal: Acs Omega / Year: 2022 Title: Structure and Enzymatic Activity of an Intellectual Disability-Associated Ornithine Decarboxylase Variant, G84R. Authors: Zhou, X.E. / Schultz, C.R. / Suino Powell, K. / Henrickson, A. / Lamp, J. / Brunzelle, J.S. / Demeler, B. / Vega, I.E. / Bachmann, A.S. / Melcher, K. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7u6p.cif.gz | 176.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7u6p.ent.gz | 138.2 KB | Display | PDB format |
PDBx/mmJSON format | 7u6p.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/u6/7u6p ftp://data.pdbj.org/pub/pdb/validation_reports/u6/7u6p | HTTPS FTP |
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-Related structure data
Related structure data | 7u6uC 7s3fS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 47341.754 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: G84R variant / Source: (gene. exp.) Homo sapiens (human) / Gene: ODC1 / Production host: Escherichia coli (E. coli) / References: UniProt: P11926, ornithine decarboxylase #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.62 Å3/Da / Density % sol: 53.14 % |
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Crystal grow | Temperature: 278 K / Method: vapor diffusion, sitting drop Details: 0.2 M sodium acetate trihydrate pH 7.0, 20% w/v polyethylene glycol 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-D / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 6, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→45.6 Å / Num. obs: 42293 / % possible obs: 99.9 % / Redundancy: 9.4 % / CC1/2: 0.993 / Net I/σ(I): 8.5 |
Reflection shell | Resolution: 2.35→2.43 Å / Rmerge(I) obs: 1.35 / Num. unique obs: 4104 / CC1/2: 0.87 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7S3F Resolution: 2.35→42.464 Å / SU ML: 0.25 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 21.5 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 106.87 Å2 / Biso mean: 37.6127 Å2 / Biso min: 10.64 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.35→42.464 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0
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