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データを開く
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基本情報
登録情報 | データベース: PDB / ID: 7ts0 | ||||||||||||||||||
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タイトル | Cryo-EM structure of corticotropin releasing factor receptor 2 bound to Urocortin 1 and coupled with heterotrimeric Go protein | ||||||||||||||||||
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![]() | SIGNALING PROTEIN / GPCR / corticotropin releasing factor receptor 2 / Urocortin 1 / Go protein | ||||||||||||||||||
機能・相同性 | ![]() histone deacetylase inhibitor activity / corticotrophin-releasing factor receptor activity / corticotropin-releasing hormone receptor activity / corticotropin-releasing hormone receptor 2 binding / positive regulation of corticotropin secretion / positive regulation of behavioral fear response / negative regulation of hormone secretion / varicosity / response to auditory stimulus / drinking behavior ...histone deacetylase inhibitor activity / corticotrophin-releasing factor receptor activity / corticotropin-releasing hormone receptor activity / corticotropin-releasing hormone receptor 2 binding / positive regulation of corticotropin secretion / positive regulation of behavioral fear response / negative regulation of hormone secretion / varicosity / response to auditory stimulus / drinking behavior / positive regulation of cAMP-mediated signaling / negative regulation of appetite / positive regulation of vascular permeability / neuropeptide hormone activity / negative regulation of cell size / G-protein activation / Activation of the phototransduction cascade / Glucagon-type ligand receptors / Thromboxane signalling through TP receptor / Sensory perception of sweet, bitter, and umami (glutamate) taste / G beta:gamma signalling through PI3Kgamma / G beta:gamma signalling through CDC42 / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / Ca2+ pathway / G alpha (z) signalling events / G protein-coupled peptide receptor activity / Vasopressin regulates renal water homeostasis via Aquaporins / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / Adrenaline,noradrenaline inhibits insulin secretion / ADP signalling through P2Y purinoceptor 12 / G alpha (q) signalling events / Class B/2 (Secretin family receptors) / G alpha (i) signalling events / Thrombin signalling through proteinase activated receptors (PARs) / alkylglycerophosphoethanolamine phosphodiesterase activity / photoreceptor outer segment membrane / response to pain / spectrin binding / negative regulation of feeding behavior / positive regulation of calcium ion import / startle response / associative learning / peptide hormone binding / social behavior / photoreceptor outer segment / corticotropin-releasing hormone receptor 1 binding / positive regulation of collagen biosynthetic process / Synthesis, secretion, and deacylation of Ghrelin / neuropeptide signaling pathway / axon terminus / response to glucocorticoid / regulation of synaptic transmission, glutamatergic / cardiac muscle cell apoptotic process / positive regulation of cardiac muscle contraction / negative regulation of blood pressure / aerobic respiration / photoreceptor inner segment / positive regulation of DNA replication / positive regulation of translation / female pregnancy / long-term synaptic potentiation / sensory perception of sound / adenylate cyclase-modulating G protein-coupled receptor signaling pathway / sensory perception of taste / vasodilation / positive regulation of interleukin-6 production / neuron projection development / heterotrimeric G-protein complex / signaling receptor complex adaptor activity / GTPase binding / positive regulation of peptidyl-serine phosphorylation / response to estradiol / retina development in camera-type eye / phospholipase C-activating G protein-coupled receptor signaling pathway / cell body / positive regulation of cytosolic calcium ion concentration / cellular response to hypoxia / G alpha (s) signalling events / positive regulation of cell growth / perikaryon / response to oxidative stress / negative regulation of neuron apoptotic process / cell population proliferation / cell surface receptor signaling pathway / G protein-coupled receptor signaling pathway / negative regulation of gene expression / GTPase activity / dendrite / protein-containing complex binding / positive regulation of transcription by RNA polymerase II / extracellular region / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||||||||||||||
生物種 | ![]() ![]() ![]() synthetic construct (人工物) | ||||||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.8 Å | ||||||||||||||||||
![]() | Zhao, L.-H. / Lin, J. / Mao, C. / Zhou, X.E. / Ji, S. / Shen, D. / Xiao, P. / Melcher, K. / Zhang, Y. / Yu, X. / Xu, H.E. | ||||||||||||||||||
資金援助 | ![]() ![]()
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![]() | ![]() タイトル: Structure insights into selective coupling of G protein subtypes by a class B G protein-coupled receptor. 著者: Li-Hua Zhao / Jingyu Lin / Su-Yu Ji / X Edward Zhou / Chunyou Mao / Dan-Dan Shen / Xinheng He / Peng Xiao / Jinpeng Sun / Karsten Melcher / Yan Zhang / Xiao Yu / H Eric Xu / ![]() ![]() 要旨: The ability to couple with multiple G protein subtypes, such as G, G, or G, by a given G protein-coupled receptor (GPCR) is critical for many physiological processes. Over the past few years, the ...The ability to couple with multiple G protein subtypes, such as G, G, or G, by a given G protein-coupled receptor (GPCR) is critical for many physiological processes. Over the past few years, the cryo-EM structures for all 15 members of the medically important class B GPCRs, all in complex with G protein, have been determined. However, no structure of class B GPCRs with G has been solved to date, limiting our understanding of the precise mechanisms of G protein coupling selectivity. Here we report the structures of corticotropin releasing factor receptor 2 (CRF2R) bound to Urocortin 1 (UCN1), coupled with different classes of heterotrimeric G proteins, G and G. We compare these structures with the structure of CRF2R in complex with G to uncover the structural differences that determine the selective coupling of G protein subtypes by CRF2R. These results provide important insights into the structural basis for the ability of CRF2R to couple with multiple G protein subtypes. | ||||||||||||||||||
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
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ダウンロード
PDBx/mmCIF形式 | ![]() | 250.3 KB | 表示 | ![]() |
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PDB形式 | ![]() | 195 KB | 表示 | ![]() |
PDBx/mmJSON形式 | ![]() | ツリー表示 | ![]() | |
その他 | ![]() |
-検証レポート
文書・要旨 | ![]() | 816.9 KB | 表示 | ![]() |
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文書・詳細版 | ![]() | 821.8 KB | 表示 | |
XML形式データ | ![]() | 34.6 KB | 表示 | |
CIF形式データ | ![]() | 53.6 KB | 表示 | |
アーカイブディレクトリ | ![]() ![]() | HTTPS FTP |
-関連構造データ
関連構造データ | ![]() 26104MC ![]() 7tryC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 ( |
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類似構造データ | 類似検索 - 機能・相同性 ![]() |
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リンク
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集合体
登録構造単位 | ![]()
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要素
-タンパク質 , 4種, 4分子 PABG
#1: タンパク質 | 分子量: 63716.246 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() 参照: UniProt: Q13324 |
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#3: タンパク質 | 分子量: 40032.488 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() 発現宿主: ![]() ![]() |
#4: タンパク質 | 分子量: 43706.750 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 発現宿主: ![]() ![]() 参照: UniProt: P54311 |
#5: タンパク質 | 分子量: 7861.143 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) ![]() ![]() 発現宿主: ![]() ![]() |
-タンパク質・ペプチド / 抗体 , 2種, 2分子 UH
#2: タンパク質・ペプチド | 分子量: 4703.277 Da / 分子数: 1 / Fragment: UNP residues 83-122 / 由来タイプ: 合成 / 由来: (合成) ![]() |
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#6: 抗体 | 分子量: 26277.299 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) synthetic construct (人工物) / 発現宿主: ![]() ![]() |
-詳細
研究の焦点であるリガンドがあるか | N |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
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試料調製
構成要素 | 名称: Corticotropin releasing factor receptor 2 (CRF2R) bound to Urocortin 1 (UCN1) and coupled with G11 proteins タイプ: COMPLEX / Entity ID: all / 由来: MULTIPLE SOURCES |
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由来(天然) | 生物種: ![]() |
由来(組換発現) | 生物種: ![]() ![]() |
緩衝液 | pH: 7.5 |
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES |
急速凍結 | 凍結剤: ETHANE |
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電子顕微鏡撮影
実験機器 | ![]() モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: ![]() |
電子レンズ | モード: BRIGHT FIELD / 最大 デフォーカス(公称値): 4000 nm / 最小 デフォーカス(公称値): 1000 nm |
撮影 | 電子線照射量: 64 e/Å2 フィルム・検出器のモデル: GATAN K2 SUMMIT (4k x 4k) |
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解析
ソフトウェア | 名称: PHENIX / バージョン: 1.13_2998: / 分類: 精密化 | ||||||||||||||||||||||||
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3次元再構成 | 解像度: 2.8 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 171435 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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