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- PDB-7tpb: p120RasGAP SH3 domain in complex with DLC1 RhoGAP domain -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 7tpb
Titlep120RasGAP SH3 domain in complex with DLC1 RhoGAP domain
Components
  • Ras GTPase-activating protein 1
  • Rho GTPase-activating protein 7
KeywordsSIGNALING PROTEIN / SH3 domain / RhoGAP domain / complex / GTPase
Function / homology
Function and homology information


negative regulation of Ras protein signal transduction / GTPase activator activity / positive regulation of GTPase activity / MAPK cascade
Similarity search - Function
Ras GTPase-activating protein 1 / RasGAP, SH3 domain / Ras GTPase-activating protein 1, C-terminal SH2 domain / Ras GTPase-activating protein / Ras GTPase-activating protein, conserved site / Ras GTPase-activating proteins domain signature. / GTPase-activator protein for Ras-like GTPase / Ras GTPase-activating proteins profile. / GTPase-activator protein for Ras-like GTPases / Ras GTPase-activating domain ...Ras GTPase-activating protein 1 / RasGAP, SH3 domain / Ras GTPase-activating protein 1, C-terminal SH2 domain / Ras GTPase-activating protein / Ras GTPase-activating protein, conserved site / Ras GTPase-activating proteins domain signature. / GTPase-activator protein for Ras-like GTPase / Ras GTPase-activating proteins profile. / GTPase-activator protein for Ras-like GTPases / Ras GTPase-activating domain / Rho GTPase activation protein / C2 domain / Protein kinase C conserved region 2 (CalB) / C2 domain / C2 domain profile. / PH domain / C2 domain superfamily / PH domain profile. / Pleckstrin homology domain. / Pleckstrin homology domain / SH3 domain / SH2 domain / Src homology 2 (SH2) domain profile. / Src homology 2 domains / SH2 domain / Src homology 3 domains / SH2 domain superfamily / SH3-like domain superfamily / Src homology 3 (SH3) domain profile. / SH3 domain / PH-like domain superfamily
Similarity search - Domain/homology
cDNA FLJ61389, highly similar to Ras GTPase-activating protein 1 / Isoform 6 of Rho GTPase-activating protein 7
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 3.2 Å
AuthorsStiegler, A.L. / Boggon, T.J. / Chau, J.E. / Vish, K.J.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)1R01NS117609 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)1R01GM138411 United States
CitationJournal: Nat Commun / Year: 2022
Title: SH3 domain regulation of RhoGAP activity: Crosstalk between p120RasGAP and DLC1 RhoGAP.
Authors: Chau, J.E. / Vish, K.J. / Boggon, T.J. / Stiegler, A.L.
History
DepositionJan 25, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 3, 2022Provider: repository / Type: Initial release
Revision 1.1Aug 24, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ras GTPase-activating protein 1
B: Rho GTPase-activating protein 7
C: Ras GTPase-activating protein 1
D: Rho GTPase-activating protein 7
E: Ras GTPase-activating protein 1
F: Rho GTPase-activating protein 7
G: Ras GTPase-activating protein 1
H: Rho GTPase-activating protein 7


Theoretical massNumber of molelcules
Total (without water)134,6468
Polymers134,6468
Non-polymers00
Water0
1
A: Ras GTPase-activating protein 1
B: Rho GTPase-activating protein 7


Theoretical massNumber of molelcules
Total (without water)33,6622
Polymers33,6622
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1610 Å2
ΔGint-6 kcal/mol
Surface area13020 Å2
MethodPISA
2
C: Ras GTPase-activating protein 1
D: Rho GTPase-activating protein 7


Theoretical massNumber of molelcules
Total (without water)33,6622
Polymers33,6622
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1600 Å2
ΔGint-6 kcal/mol
Surface area13220 Å2
MethodPISA
3
E: Ras GTPase-activating protein 1
F: Rho GTPase-activating protein 7


Theoretical massNumber of molelcules
Total (without water)33,6622
Polymers33,6622
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1640 Å2
ΔGint-7 kcal/mol
Surface area13060 Å2
MethodPISA
4
G: Ras GTPase-activating protein 1
H: Rho GTPase-activating protein 7


Theoretical massNumber of molelcules
Total (without water)33,6622
Polymers33,6622
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1560 Å2
ΔGint-6 kcal/mol
Surface area12880 Å2
MethodPISA
Unit cell
Length a, b, c (Å)143.745, 143.745, 152.776
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number146
Space group name H-MH3
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and resid 283 through 339)
21(chain C and resid 283 through 339)
31(chain E and resid 283 through 339)
41(chain G and resid 283 through 339)
12(chain B and (resid 1070 through 1088 or resid 1090...
22(chain D and (resid 1070 through 1088 or resid 1090...
32(chain F and (resid 1070 through 1088 or resid 1090...
42(chain H and (resid 1070 through 1088 or resid 1090...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111ARGARGVALVAL(chain A and resid 283 through 339)AA283 - 3398 - 64
211ARGARGVALVAL(chain C and resid 283 through 339)CC283 - 3398 - 64
311ARGARGVALVAL(chain E and resid 283 through 339)EE283 - 3398 - 64
411ARGARGVALVAL(chain G and resid 283 through 339)GG283 - 3398 - 64
112TYRTYRGLYGLY(chain B and (resid 1070 through 1088 or resid 1090...BB1070 - 108815 - 33
122PROPROSERSER(chain B and (resid 1070 through 1088 or resid 1090...BB1090 - 112035 - 65
132ILEILEILEILE(chain B and (resid 1070 through 1088 or resid 1090...BB112267
142ALAALALEULEU(chain B and (resid 1070 through 1088 or resid 1090...BB1124 - 123969 - 184
152PROPROCYSCYS(chain B and (resid 1070 through 1088 or resid 1090...BB1258 - 1280203 - 225
212TYRTYRGLYGLY(chain D and (resid 1070 through 1088 or resid 1090...DD1070 - 108815 - 33
222PROPROSERSER(chain D and (resid 1070 through 1088 or resid 1090...DD1090 - 112035 - 65
232ILEILEILEILE(chain D and (resid 1070 through 1088 or resid 1090...DD112267
242ALAALACYSCYS(chain D and (resid 1070 through 1088 or resid 1090...DD1124 - 128069 - 225
312TYRTYRGLYGLY(chain F and (resid 1070 through 1088 or resid 1090...FF1070 - 108815 - 33
322PROPROSERSER(chain F and (resid 1070 through 1088 or resid 1090...FF1090 - 112035 - 65
332ILEILEILEILE(chain F and (resid 1070 through 1088 or resid 1090...FF112267
342ALAALACYSCYS(chain F and (resid 1070 through 1088 or resid 1090...FF1124 - 128069 - 225
412TYRTYRGLYGLY(chain H and (resid 1070 through 1088 or resid 1090...HH1070 - 108815 - 33
422PROPROSERSER(chain H and (resid 1070 through 1088 or resid 1090...HH1090 - 112035 - 65
432ILEILEILEILE(chain H and (resid 1070 through 1088 or resid 1090...HH112267
442ALAALACYSCYS(chain H and (resid 1070 through 1088 or resid 1090...HH1124 - 128069 - 225

NCS ensembles :
ID
1
2

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Components

#1: Protein
Ras GTPase-activating protein 1


Mass: 7622.630 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Plasmid: pET / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B4DTL8
#2: Protein
Rho GTPase-activating protein 7 / Deleted in liver cancer 1 protein / DLC-1 / HP protein / Rho-type GTPase-activating protein 7 / ...Deleted in liver cancer 1 protein / DLC-1 / HP protein / Rho-type GTPase-activating protein 7 / START domain-containing protein 12 / StARD12 / StAR-related lipid transfer protein 12


Mass: 26038.930 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: DLC1, ARHGAP7, KIAA1723, STARD12 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q96QB1-6

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.26 Å3/Da / Density % sol: 45.48 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.4 / Details: 0.2 M Magnesium nitrate, 16.5% PEG 3350 / Temp details: ambient temperature

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER2 S 16M / Detector: PIXEL / Date: Jul 11, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 3.2→50 Å / Num. obs: 19702 / % possible obs: 100 % / Redundancy: 9.1 % / Biso Wilson estimate: 80.44 Å2 / Rmerge(I) obs: 0.256 / Rpim(I) all: 0.09 / Rrim(I) all: 0.272 / Χ2: 1.055 / Net I/σ(I): 5.3 / Num. measured all: 179535
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
3.2-3.317.81.719520.3320.6571.8241.038100
3.31-3.458.61.07719960.6710.3911.1461.04100
3.45-3.68.90.79619570.8410.2820.8451.049100
3.6-3.799.90.53919800.9040.180.5691.0899.9
3.79-4.039.80.41419510.9550.1390.4371.084100
4.03-4.349.30.31519720.970.1090.3341.075100
4.34-4.788.40.23319770.9750.0850.2491.041100
4.78-5.478.60.19119770.9810.0690.2041.058100
5.47-6.8910.40.17719740.9910.0580.1861.02799.9
6.89-509.20.12219660.9920.0420.131.04899.8

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
SCALEPACKdata scaling
PHASER2.8.2phasing
PHENIX1.19.2refinement
PDB_EXTRACT3.27data extraction
HKL-20002.3.8data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3KUQ, 2J05

3kuq

2j05


Resolution: 3.2→48.25 Å / SU ML: 0.44 / Cross valid method: THROUGHOUT / σ(F): 1.98 / Phase error: 31.22 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2597 920 4.74 %
Rwork0.2125 18492 -
obs0.2149 19412 99.93 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 201.34 Å2 / Biso mean: 85.5158 Å2 / Biso min: 41.43 Å2
Refinement stepCycle: final / Resolution: 3.2→48.25 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8096 0 0 0 8096
Num. residues----1010
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A684X-RAY DIFFRACTION2.414TORSIONAL
12C684X-RAY DIFFRACTION2.414TORSIONAL
13E684X-RAY DIFFRACTION2.414TORSIONAL
14G684X-RAY DIFFRACTION2.414TORSIONAL
21B2252X-RAY DIFFRACTION2.414TORSIONAL
22D2252X-RAY DIFFRACTION2.414TORSIONAL
23F2252X-RAY DIFFRACTION2.414TORSIONAL
24H2252X-RAY DIFFRACTION2.414TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 7 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all
3.2-3.370.33221220.300726582780
3.37-3.580.31871310.26726482779
3.58-3.860.27141250.233426332758
3.86-4.240.26431270.216426672794
4.24-4.860.26481270.185126342761
4.86-6.110.26041420.221626252767
6.12-48.250.21961460.177626272773
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
15.67433.73772.12337.02672.93821.5412-1.74423.0903-0.0837-1.72231.0848-1.64880.07460.09170.30480.6417-0.331-0.02290.9805-0.49130.68447.834256.007297.62
24.2722-5.38860.17145.5810.59328.79890.1466-0.55650.20480.68150.79780.17740.41950.5618-0.8070.6811-0.1024-0.11960.63560.03960.71893.448559.5374102.3639
34.8944-1.4106-0.34743.76421.32572.9373-0.5342-1.0403-0.54290.21850.07980.18320.35240.40540.31730.6049-0.0128-0.10460.80380.02970.58584.464455.7971102.651
45.32880.2051-5.11589.59483.81598.33820.28880.74090.15331.1466-0.06040.6181-0.4654-0.0879-0.20090.6975-0.05870.00260.75950.15930.5032-30.081472.1401113.0788
56.8277-2.3767-1.34835.479-0.11485.3237-0.30020.31040.3869-0.45070.35720.38190.268-0.35920.07050.4468-0.0941-0.12580.6739-0.01740.3478-22.75165.421396.4412
62.28550.2387-3.05566.48642.89388.98690.00091.00940.3531-0.5245-0.09650.2092-1.56440.02790.20750.7798-0.1228-0.19390.75110.02980.4117-24.577269.030198.7736
75.1211-6.10082.34198.57770.07464.634-0.44250.1139-0.37641.12910.3785-0.76630.17110.25380.01810.6288-0.0797-0.15380.60350.07780.6158-11.449560.5357119.2074
81.95370.36042.17936.87760.90765.4059-1.16180.3177-3.11190.33650.86610.08480.4796-0.9801-0.25411.1922-0.0941-0.1520.6990.04670.7649-14.337149.2398121.1649
95.3419-0.67040.55064.63121.39652.67940.47530.1103-0.51660.0078-0.0862-0.20440.568-0.0501-0.26480.6725-0.1414-0.18670.38810.04060.3939-15.526357.9289106.5778
104.3543-3.47132.16933.2485-0.93262.97151.17290.4715-0.98622.0765-0.3775-0.91160.56090.9561-0.60860.6614-0.1343-0.19730.7263-0.00780.5014-7.753455.7112113.2531
115.79570.7196.02938.04251.21276.43890.4159-1.4282.25981.1216-1.22251.34951.2144-0.45020.85221.0075-0.17380.42760.98080.07531.1084-27.022772.061154.0928
123.7082-3.23372.66971.9487-2.47856.3118-0.2784-0.31031.05620.4171-0.04090.26510.1708-0.31580.32960.6827-0.02540.03770.4661-0.15810.9702-20.457870.846151.0509
139.047-2.4387-4.85763.04181.00286.28640.550.18130.7706-0.2047-0.60550.48241.4154-0.5215-0.05590.8528-0.0881-0.09840.64680.14020.5569-24.12569.2524150.8132
142.025-1.2972-1.17199.83273.90352.1248-0.4589-0.48151.1484-1.11480.0942-0.2963-0.9046-0.13180.24130.69160.1149-0.22080.8143-0.13350.825410.331452.95139.4324
157.8234-0.95480.64175.4091.52875.63750.07950.08850.06260.9182-0.1873-0.67770.61160.4030.04860.94250.0484-0.33760.45940.00640.59020.984954.1372156.3654
164.60932.5628-2.72495.77820.33014.1404-0.9346-1.32531.1733-1.18481.6831-1.87570.70160.8015-0.53091.39940.045-0.23310.8927-0.07520.470610.514450.9975156.3866
174.60731.1282-3.16966.51351.14859.48840.09180.56330.13340.4327-0.41170.2353-1.3449-0.09630.39670.8638-0.0084-0.29240.47920.02490.5486-0.389558.8576146.9388
184.9686-3.3082-2.24955.56590.74549.1244-0.0171.0797-0.3025-0.8382-0.02040.8440.457-0.0732-0.01381.0618-0.1085-0.39030.6480.15990.6919-17.079754.7927132.1116
194.59911.0548-0.13256.3465-2.23691.9070.34633.129-1.303-0.4146-1.35070.15523.22410.27760.38591.2418-0.5607-0.272-1.1832-0.27350.4289-7.899547.7746146.5027
203.0581-1.8826-3.24353.0953-1.30077.75540.7234-0.47920.42520.4412-0.2618-0.0742-0.1361-0.4805-0.1120.7486-0.1336-0.20770.5420.0050.7036-11.62562.1187146.395
219.2299-2.88981.58551.3446-1.93788.14660.0749-0.06230.1965-1.67380.44290.3756-0.3084-1.636-0.63251.23460.03-0.17650.57020.13890.5138-16.742457.9394140.3162
226.4337-0.6814-4.13023.4007-2.11874.1903-0.0906-1.79331.5089-0.4843-1.5133-1.65761.62162.7988-0.09190.5465-0.3155-0.34910.5928-0.58061.014926.680111.4661128.2253
236.2498-0.2327-0.37094.95681.24863.9312-0.06251.5695-2.004-0.26361.10820.6074-0.48930.9167-1.02820.6773-0.0855-0.04040.8154-0.12841.29320.235912.7378124.696
245.1181-5.4365-0.75495.46810.56776.34650.6131.0021-0.2734-0.1547-0.7249-0.64040.9790.00230.11480.7009-0.01210.07670.6266-0.15510.858423.786914.4224124.5332
253.6352.52261.67854.41491.22673.9143-0.32241.8153-0.8188-1.61170.17590.3815-0.10540.26360.1540.73570.10690.08040.66070.0860.6703-13.257925.1966115.6894
262.30170.20013.06924.1593-3.10556.95480.43071.16060.1714-0.9933-0.58080.6426-0.1294-0.21650.30831.0390.254-0.0811.1412-0.14440.5821-9.470733.4335112.1857
272.5792-0.12223.03775.22530.64345.07470.238-0.12870.9287-0.1493-0.31340.1556-0.8190.27680.27160.833-0.00230.07870.59240.0460.4255-2.781135.7189127.7499
282.1688-1.15011.89625.2501-0.88296.4421-0.477-0.9329-2.0618-0.38850.12370.03130.4318-0.102-0.03520.6380.0105-0.0460.5129-0.05980.1547-0.938824.3667131.95
294.7204-0.24892.56891.6998-3.04128.32490.15780.18540.1022-0.0968-0.12530.1247-0.3863-0.4898-0.00550.80780.12020.03890.3631-0.00090.438-4.084926.854124.1835
304.6601-2.3630.74512.1712-1.99413.2812-0.40932.9658-2.6945-0.98170.7589-1.24841.12312.180.59141.1582-0.00250.42931.4492-0.5440.982416.937424.4619105.78
317.3219-8.39517.02941.7337-5.61339.1801-1.17681.47511.77291.7957-0.292-3.3733-0.38130.0404-0.3981.4577-0.38290.28790.8755-0.20420.181816.113534.0412106.761
328.295-1.5025.77443.29340.32358.4264-0.3102-0.45350.7975-0.2699-0.5616-0.3298-1.1519-0.5740.63790.7483-0.15980.17320.5356-0.07620.54927.23835.7265120.9382
338.8972.58652.39522.7238-2.36767.8650.4803-0.5898-0.1638-0.2107-0.2857-0.9281-1.03320.1285-0.42910.45840.0217-0.0830.6762-0.04560.535111.151221.3784120.351
344.21872.15071.44256.83091.03137.8274-0.25640.4357-1.3219-1.45171.8565-2.0277-0.19280.6267-1.19920.8362-0.060.15860.8854-0.23290.647816.31924.0359114.1945
357.1634-3.3859-1.84214.0739-0.19840.9273.10921.62221.4267-1.2725-1.1691-1.14-0.6756-1.20740.39321.03630.0992-0.01860.86680.09411.2135-12.221131.128977.2769
368.2845-6.14131.35587.31071.71945.67271.38190.92912.1962-0.8876-0.5877-3.1111-0.22981.67851.23431.1454-0.71950.37671.25350.28051.3324-3.338922.458767.2428
373.18321.0127-1.4519.0669-6.24714.19150.7753-0.4362-0.99431.09290.6923.029-0.4316-0.9585-0.96771.0925-0.0442-0.04060.6862-0.13311.205-1.299619.623473.8391
384.5542-3.37374.21924.5701-0.44738.087-1.0058-0.19950.063-0.10.81070.1293-0.4418-0.44990.22290.8541-0.09650.24130.70040.0540.813-4.879327.998277.6427
394.9621.4512.9471.9857-2.92553.25830.83560.18211.01891.075-0.97741.766-0.48110.98050.38790.9917-0.11570.04391.377-0.25870.650227.76016.137186.3259
407.62874.55566.74563.82544.18366.01691.07341.786-0.5361.8480.2288-0.70980.9720.8449-0.78520.7615-0.05710.08771.3017-0.24860.902631.778614.728289.5919
418.9441-0.6982-1.93276.08340.1835.9674-0.3612-0.86790.479-0.18560.5915-0.8433-0.58012.5750.19040.5278-0.2490.21340.9718-0.29350.651829.318521.239474.0191
4210.01530.3146-1.18034.4283-2.6466.9303-0.34061.1549-1.593-1.13090.1097-0.79370.32780.0301-0.00590.5668-0.19180.21640.7807-0.33080.702619.362615.386369.7997
434.9516-4.88065.11684.7585-4.36289.32060.15450.0583-1.64561.7874-0.1850.1293-0.51142.13140.02351.0195-0.13050.20371.2537-0.01340.85931.483211.891871.5689
445.3701-0.549-6.10694.2414-1.95877.6568-1.1806-0.4836-1.0006-0.16250.06660.15381.17570.22960.57350.5295-0.19810.22360.6871-0.20360.848818.664215.831880.7803
458.8081-2.98551.11011.7866-3.28228.4461-1.2354-1.1750.684-0.22881.1926-0.4068-0.8588-0.25230.05581.2243-0.01790.19780.8014-0.29030.715511.283831.611995.1631
465.9693-0.97520.23023.7711-4.31694.2597-0.8712-0.30090.35810.3637-1.1406-1.23040.10611.6141.84461.0981-0.25290.10670.8305-0.0460.688922.576728.86680.7622
478.2081-2.3413.87196.53870.88687.070.3041-0.0117-0.0824-0.2307-0.42510.9384-1.22330.2585-0.15590.7083-0.06350.18620.4621-0.24150.6549.151222.425281.1332
487.3366-1.11010.94918.48991.53747.25590.537-0.7103-0.54741.8297-0.7593-0.1859-1.5549-1.1882-0.03291.15310.0580.21370.792-0.06760.44547.716628.04787.2229
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid 282 through 291 )A282 - 291
2X-RAY DIFFRACTION2chain 'A' and (resid 292 through 313 )A292 - 313
3X-RAY DIFFRACTION3chain 'A' and (resid 314 through 339 )A314 - 339
4X-RAY DIFFRACTION4chain 'B' and (resid 1070 through 1092 )B0
5X-RAY DIFFRACTION5chain 'B' and (resid 1093 through 1129 )B0
6X-RAY DIFFRACTION6chain 'B' and (resid 1130 through 1155 )B0
7X-RAY DIFFRACTION7chain 'B' and (resid 1156 through 1176 )B0
8X-RAY DIFFRACTION8chain 'B' and (resid 1177 through 1194 )B0
9X-RAY DIFFRACTION9chain 'B' and (resid 1195 through 1259 )B0
10X-RAY DIFFRACTION10chain 'B' and (resid 1260 through 1280 )B0
11X-RAY DIFFRACTION11chain 'C' and (resid 281 through 291 )C281 - 291
12X-RAY DIFFRACTION12chain 'C' and (resid 292 through 313 )C292 - 313
13X-RAY DIFFRACTION13chain 'C' and (resid 314 through 339 )C314 - 339
14X-RAY DIFFRACTION14chain 'D' and (resid 1070 through 1092 )D0
15X-RAY DIFFRACTION15chain 'D' and (resid 1093 through 1129 )D0
16X-RAY DIFFRACTION16chain 'D' and (resid 1130 through 1142 )D0
17X-RAY DIFFRACTION17chain 'D' and (resid 1143 through 1163 )D0
18X-RAY DIFFRACTION18chain 'D' and (resid 1164 through 1194 )D0
19X-RAY DIFFRACTION19chain 'D' and (resid 1195 through 1218 )D0
20X-RAY DIFFRACTION20chain 'D' and (resid 1219 through 1259 )D0
21X-RAY DIFFRACTION21chain 'D' and (resid 1260 through 1282 )D0
22X-RAY DIFFRACTION22chain 'E' and (resid 281 through 291 )E281 - 291
23X-RAY DIFFRACTION23chain 'E' and (resid 292 through 313 )E292 - 313
24X-RAY DIFFRACTION24chain 'E' and (resid 314 through 339 )E314 - 339
25X-RAY DIFFRACTION25chain 'F' and (resid 1070 through 1079 )F0
26X-RAY DIFFRACTION26chain 'F' and (resid 1080 through 1092 )F0
27X-RAY DIFFRACTION27chain 'F' and (resid 1093 through 1105 )F0
28X-RAY DIFFRACTION28chain 'F' and (resid 1106 through 1129 )F0
29X-RAY DIFFRACTION29chain 'F' and (resid 1130 through 1163 )F0
30X-RAY DIFFRACTION30chain 'F' and (resid 1164 through 1178 )F0
31X-RAY DIFFRACTION31chain 'F' and (resid 1179 through 1194 )F0
32X-RAY DIFFRACTION32chain 'F' and (resid 1195 through 1218 )F0
33X-RAY DIFFRACTION33chain 'F' and (resid 1219 through 1259 )F0
34X-RAY DIFFRACTION34chain 'F' and (resid 1260 through 1280 )F0
35X-RAY DIFFRACTION35chain 'G' and (resid 281 through 286 )G281 - 286
36X-RAY DIFFRACTION36chain 'G' and (resid 287 through 291 )G287 - 291
37X-RAY DIFFRACTION37chain 'G' and (resid 292 through 305 )G292 - 305
38X-RAY DIFFRACTION38chain 'G' and (resid 306 through 339 )G306 - 339
39X-RAY DIFFRACTION39chain 'H' and (resid 1070 through 1079 )H0
40X-RAY DIFFRACTION40chain 'H' and (resid 1080 through 1092 )H0
41X-RAY DIFFRACTION41chain 'H' and (resid 1093 through 1105 )H0
42X-RAY DIFFRACTION42chain 'H' and (resid 1106 through 1129 )H0
43X-RAY DIFFRACTION43chain 'H' and (resid 1130 through 1142 )H0
44X-RAY DIFFRACTION44chain 'H' and (resid 1143 through 1163 )H0
45X-RAY DIFFRACTION45chain 'H' and (resid 1164 through 1194 )H0
46X-RAY DIFFRACTION46chain 'H' and (resid 1195 through 1218 )H0
47X-RAY DIFFRACTION47chain 'H' and (resid 1219 through 1259 )H0
48X-RAY DIFFRACTION48chain 'H' and (resid 1260 through 1280 )H0

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