ジャーナル: Science / 年: 2022 タイトル: Structural basis of SARS-CoV-2 Omicron immune evasion and receptor engagement. 著者: Matthew McCallum / Nadine Czudnochowski / Laura E Rosen / Samantha K Zepeda / John E Bowen / Alexandra C Walls / Kevin Hauser / Anshu Joshi / Cameron Stewart / Josh R Dillen / Abigail E ...著者: Matthew McCallum / Nadine Czudnochowski / Laura E Rosen / Samantha K Zepeda / John E Bowen / Alexandra C Walls / Kevin Hauser / Anshu Joshi / Cameron Stewart / Josh R Dillen / Abigail E Powell / Tristan I Croll / Jay Nix / Herbert W Virgin / Davide Corti / Gyorgy Snell / David Veesler / 要旨: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant of concern evades antibody-mediated immunity that comes from vaccination or infection with earlier variants due to ...The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant of concern evades antibody-mediated immunity that comes from vaccination or infection with earlier variants due to accumulation of numerous spike mutations. To understand the Omicron antigenic shift, we determined cryo-electron microscopy and x-ray crystal structures of the spike protein and the receptor-binding domain bound to the broadly neutralizing sarbecovirus monoclonal antibody (mAb) S309 (the parent mAb of sotrovimab) and to the human ACE2 receptor. We provide a blueprint for understanding the marked reduction of binding of other therapeutic mAbs that leads to dampened neutralizing activity. Remodeling of interactions between the Omicron receptor-binding domain and human ACE2 likely explains the enhanced affinity for the host receptor relative to the ancestral virus.
A: Spike glycoprotein B: Spike glycoprotein C: Spike glycoprotein D: S2L20 Fab light chain E: S2L20 Fab heavy chain F: S2L20 Fab light chain G: S2L20 Fab heavy chain H: S2L20 Fab light chain I: S2L20 Fab heavy chain J: S309 Fab Heavy Chain K: S309 Fab light chain L: S309 Fab Heavy Chain M: S309 Fab light chain N: S309 Fab Heavy Chain O: S309 Fab light chain ヘテロ分子
There is an insertion of three residues EPE between residues R214 and D215 that are numbered E9215, ...There is an insertion of three residues EPE between residues R214 and D215 that are numbered E9215, P9216, E9217.