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- PDB-7t25: OspA-Fab 319-44 complex structure -

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Basic information

Entry
Database: PDB / ID: 7t25
TitleOspA-Fab 319-44 complex structure
Components
  • 319-44 Fab heavy chain
  • 319-44 Fab light chain
  • Outer surface protein A
KeywordsIMMUNE SYSTEM / human monoclonal antibody
Function / homologyOuter surface lipoprotein, Borrelia / Outer surface lipoprotein domain superfamily / Borrelia lipoprotein / cell outer membrane / Prokaryotic membrane lipoprotein lipid attachment site profile. / cell surface / membrane / IODIDE ION / Outer surface protein A
Function and homology information
Biological speciesHomo sapiens (human)
Borreliella burgdorferi B31 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.25 Å
AuthorsRudolph, M.J.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)75N93019C00040 United States
CitationJournal: To Be Published
Title: Structural analysis of OspA-31944 complex
Authors: Rudolph, M.J.
History
DepositionDec 3, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Dec 14, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: 319-44 Fab heavy chain
L: 319-44 Fab light chain
A: 319-44 Fab heavy chain
B: 319-44 Fab light chain
E: Outer surface protein A
C: Outer surface protein A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)152,34843
Polymers150,1806
Non-polymers2,16737
Water16,322906
1
H: 319-44 Fab heavy chain
L: 319-44 Fab light chain
E: Outer surface protein A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)76,08820
Polymers75,0903
Non-polymers99817
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
A: 319-44 Fab heavy chain
B: 319-44 Fab light chain
C: Outer surface protein A
hetero molecules


Theoretical massNumber of molelcules
Total (without water)76,25923
Polymers75,0903
Non-polymers1,16920
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)254.208, 44.117, 143.219
Angle α, β, γ (deg.)90.000, 101.230, 90.000
Int Tables number5
Space group name H-MC121
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain C and (resid 24 through 25 or resid 28...
21(chain E and (resid 24 through 221 or resid 223 through 273))
12chain B
22(chain L and resid 1 through 213)
13(chain A and (resid 2 through 142 or resid 144...
23(chain H and (resid 2 through 142 or resid 144...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
111LEULEUASPASP(chain C and (resid 24 through 25 or resid 28...CF24 - 257 - 8
121ASNASNVALVAL(chain C and (resid 24 through 25 or resid 28...CF28 - 5811 - 41
131LEULEUASPASP(chain C and (resid 24 through 25 or resid 28...CF61 - 10544 - 88
141LEULEULYSLYS(chain C and (resid 24 through 25 or resid 28...CF24 - 2737 - 256
151LYSLYSSERSER(chain C and (resid 24 through 25 or resid 28...CF125 - 221108 - 204
161LEULEULYSLYS(chain C and (resid 24 through 25 or resid 28...CF223 - 273206 - 256
211LEULEUSERSER(chain E and (resid 24 through 221 or resid 223 through 273))EE24 - 2217 - 204
221LEULEULYSLYS(chain E and (resid 24 through 221 or resid 223 through 273))EE223 - 273206 - 256
112GLUGLUGLYGLYchain BBD1 - 2131 - 213
212GLUGLUGLYGLY(chain L and resid 1 through 213)LB1 - 2131 - 213
113METMETGLYGLY(chain A and (resid 2 through 142 or resid 144...AC2 - 1422 - 142
123LEULEUPROPRO(chain A and (resid 2 through 142 or resid 144...AC144 - 152144 - 152
133THRTHRVALVAL(chain A and (resid 2 through 142 or resid 144...AC154 - 155154 - 155
143TRPTRPPROPRO(chain A and (resid 2 through 142 or resid 144...AC157 - 216157 - 216
213METMETGLYGLY(chain H and (resid 2 through 142 or resid 144...HA2 - 1422 - 142
223LEULEUPROPRO(chain H and (resid 2 through 142 or resid 144...HA144 - 152144 - 152
233THRTHRTHRTHR(chain H and (resid 2 through 142 or resid 144...HA154154

NCS ensembles :
ID
1
2
3

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Components

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Antibody , 2 types, 4 molecules HALB

#1: Antibody 319-44 Fab heavy chain


Mass: 24192.314 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)
#2: Antibody 319-44 Fab light chain


Mass: 23302.824 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Cricetulus griseus (Chinese hamster)

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Protein / Sugars , 2 types, 4 molecules EC

#3: Protein Outer surface protein A


Mass: 27595.053 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Borreliella burgdorferi B31 (bacteria) / Strain: ATCC 35210 / B31 / CIP 102532 / DSM 4680 / Gene: ospA, BB_A15 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P0CL66
#5: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 4 types, 941 molecules

#4: Chemical...
ChemComp-CL / CHLORIDE ION / Chloride


Mass: 35.453 Da / Num. of mol.: 29 / Source method: obtained synthetically / Formula: Cl
#6: Chemical
ChemComp-IOD / IODIDE ION / Iodide


Mass: 126.904 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: I
#7: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C2H6O2
#8: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 906 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.62 Å3/Da / Density % sol: 53.1 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 200 mM ammonium iodide and 20% PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.979 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jun 26, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 2.25→50 Å / Num. obs: 74086 / % possible obs: 96.4 % / Redundancy: 4.2 % / Rmerge(I) obs: 0.132 / Rpim(I) all: 0.074 / Rrim(I) all: 0.152 / Χ2: 1.071 / Net I/σ(I): 6 / Num. measured all: 307815
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. unique obsCC1/2Rpim(I) allRrim(I) allΧ2% possible all
2.25-2.293.80.79636460.320.4720.9320.53995.3
2.29-2.3340.66936750.5850.3820.7760.47396
2.33-2.384.10.57836160.6070.330.670.42696.3
2.38-2.424.10.52537020.6890.2980.6080.44697.2
2.42-2.484.10.47936930.7060.2710.5540.47896.2
2.48-2.534.20.42936100.7420.2440.4970.48696.4
2.53-2.64.10.36837080.8180.210.4260.53396.3
2.6-2.674.10.33936470.8340.1940.3930.58796.8
2.67-2.753.90.29237030.8720.1710.3410.65396.4
2.75-2.8340.24136500.9080.1380.280.70995.4
2.83-2.9440.20836140.9320.1210.2420.79194.7
2.94-3.053.60.17535080.960.1060.2060.95692.1
3.05-3.1940.15237470.9670.0890.1771.1396.7
3.19-3.364.50.12937620.980.0710.1481.33998.6
3.36-3.574.40.10937540.9840.060.1261.65298
3.57-3.854.40.09637820.980.0530.1111.86498
3.85-4.234.30.08237480.990.0450.0941.93896.9
4.23-4.854.20.06937790.9940.0390.081.98296.6
4.85-6.14.10.06836790.9940.0370.0781.86594
6.1-505.10.06340630.9960.0310.0711.76699.5

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Phasing

PhasingMethod: molecular replacement

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Processing

Software
NameVersionClassification
SCALEPACKdata scaling
PHASERphasing
PHENIX1.11.1_2575refinement
PDB_EXTRACT3.27data extraction
DENZOdata reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 4ZD3, 1FJ1
Resolution: 2.25→46.949 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 2.09 / Phase error: 24.02 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2326 3576 4.95 %
Rwork0.1912 68734 -
obs0.1933 72310 96.14 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 123.06 Å2 / Biso mean: 37.45 Å2 / Biso min: 10.54 Å2
Refinement stepCycle: final / Resolution: 2.25→46.949 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms10189 0 66 906 11161
Biso mean--41.72 37.79 -
Num. residues----1340
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00410433
X-RAY DIFFRACTIONf_angle_d0.69414133
X-RAY DIFFRACTIONf_chiral_restr0.0481649
X-RAY DIFFRACTIONf_plane_restr0.0041777
X-RAY DIFFRACTIONf_dihedral_angle_d13.1236335
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11C2265X-RAY DIFFRACTION7.274TORSIONAL
12E2265X-RAY DIFFRACTION7.274TORSIONAL
21B2035X-RAY DIFFRACTION7.274TORSIONAL
22L2035X-RAY DIFFRACTION7.274TORSIONAL
31A1888X-RAY DIFFRACTION7.274TORSIONAL
32H1888X-RAY DIFFRACTION7.274TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.25-2.27960.29441290.2421245690
2.2796-2.31080.30411420.2381265996
2.3108-2.34390.30061270.2222263997
2.3439-2.37880.26321360.2219258096
2.3788-2.4160.27021350.2258263798
2.416-2.45560.26931280.2156264896
2.4556-2.4980.29561320.2217258997
2.498-2.54340.29341260.229262696
2.5434-2.59230.28451340.2231269597
2.5923-2.64520.24161360.2204256896
2.6452-2.70270.30771290.2179266996
2.7027-2.76560.26261170.2072265496
2.7656-2.83470.22741730.2038251595
2.8347-2.91140.2461270.2036262795
2.9114-2.9970.23511150.2118256393
2.997-3.09370.26871490.2167253994
3.0937-3.20430.26161460.2068266298
3.2043-3.33250.24951320.1979274699
3.3325-3.48420.2211390.185266798
3.4842-3.66780.23561420.1769273798
3.6678-3.89750.20531410.166265598
3.8975-4.19820.19281580.1575266197
4.1982-4.62040.17271450.1415267696
4.6204-5.28820.17241500.1446270097
5.2882-6.65950.20941340.1875264894
6.6595-46.9490.22671540.2052291899
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
18.67150.15715.20162.33351.54047.3083-0.1217-0.07840.31660.06450.1401-0.2378-0.3559-0.10430.00860.1688-0.00660.02340.1028-0.01330.214567.669226.07648.8578
24.4709-0.3532-1.23643.1135-1.32641.607-0.08540.14060.14620.04270.10210.0134-0.05220.01030.00850.1539-0.01070.01680.10190.01920.128872.741214.766651.0308
36.033-0.4533-1.07356.3805-0.62915.65380.13210.7113-0.2021-0.3184-0.2457-0.37470.56430.43140.12660.2147-0.01980.07410.17890.04520.223278.838714.743143.0362
43.348-1.56411.20251.7525-0.97122.6770.05760.08430.0483-0.01090.0073-0.0565-0.05490.0309-0.10260.1583-0.0390.01780.121-0.01330.152570.67719.426849.1135
53.8868-0.5108-2.13540.5984-0.60534.64080.01090.07890.01020.0342-0.03490.0373-0.04750.19720.04550.1512-0.0135-0.00990.09050.00330.170939.584423.833348.1713
69.4554.7217-3.65464.3559-3.56464.4944-0.2993-0.2873-0.25120.2803-0.11770.43420.1288-0.62140.22480.19710.0013-0.02280.193-0.00730.170129.381921.376855.4401
77.53251.7973-0.83835.53140.24225.2821-0.1205-0.02370.3076-0.0618-0.04250.4579-0.3809-0.46910.22810.27190.05460.00950.1425-0.01330.26733.637630.391450.6083
87.6876-3.9903-3.97433.16152.56935.71170.14610.1076-0.66220.058-0.22750.423-0.1833-0.60550.06380.1898-0.0468-0.00860.1620.02010.341457.171-3.469855.8594
93.19250.3327-1.16843.1225-0.30373.95790.0203-0.1185-0.08280.0834-0.00790.006-0.0146-0.0577-0.01760.14360.00330.00720.10850.02050.183865.35180.828358.957
102.49011.08621.59690.46660.71121.02220.0251-0.5252-0.1290.1372-0.04850.1376-0.0281-0.02230.00910.21260.03370.00510.2920.08810.301541.67660.218658.7031
112.4104-1.75761.52394.3711-4.22597.4337-0.07970.2375-0.03210.2049-0.0051-0.1124-0.2622-0.01320.11170.1383-0.0620.00590.1419-0.01740.206332.297912.946643.8116
123.8415-1.57694.71593.8028-0.68536.24920.08560.1361-0.4762-0.0979-0.17580.3420.4513-0.20850.06030.269-0.0740.10610.2795-0.05430.380335.85519.269735.7204
133.1006-1.90563.89163.7549-4.52057.5477-0.19950.03490.0216-0.0172-0.0687-0.0817-0.26670.00470.28950.17450.02220.08870.1546-0.03230.200233.575611.639743.3453
146.0213-6.27765.40118.5662-4.85655.64650.1495-0.0294-0.5925-0.23150.25190.66740.2124-0.3431-0.48120.1693-0.05570.06040.23940.02810.272925.21225.874242.9219
156.3518-0.57115.18883.7852-0.56468.2598-0.02520.37310.25090.2456-0.0188-0.2558-0.4040.20010.05240.1454-0.03240.02790.1474-0.00810.221546.187145.832316.938
162.15190.70441.02542.37830.04231.8562-0.0481-0.05850.10670.03770.0108-0.06680.07640.05170.02890.13990.0587-0.00270.1786-0.0180.164641.090438.962520.6067
176.3695-1.63192.56864.5788-1.55446.0205-0.0964-0.5681-0.45680.28810.20730.41270.2477-0.3848-0.11650.17910.01420.08920.183-0.01490.201333.689535.294126.6565
182.96621.06071.29382.32551.5433.06460.0003-0.00790.11320.0570.0847-0.08440.10280.0422-0.06690.16620.03360.01950.11110.01210.176842.00340.100420.9044
195.8110.9477-0.82263.05380.70383.93860.2067-0.82470.5878-0.1410.1379-0.1973-0.07340.3054-0.27290.1722-0.0009-0.05340.13150.00890.093974.298348.363125.7962
203.15090.1668-2.61562.09960.63685.72290.0460.04840.007-0.1254-0.06780.0146-0.0299-0.31180.01830.12360.0157-0.00840.14520.0220.203672.123444.472620.2661
216.3863-2.89850.07074.13912.08932.97450.24470.4640.0338-0.4666-0.19-0.5918-0.1440.7553-0.04010.24890.05610.03210.27710.0460.235483.100242.81514.7082
224.1676-4.8349-2.27917.10671.96133.15190.0602-0.02140.9126-0.35060.085-0.685-0.31030.1697-0.1910.2889-0.00660.04070.2060.06870.247674.938653.221717.719
236.60121.8163-0.27283.1173-2.01972.0430.1362-0.0275-0.487-0.0056-0.0077-0.5317-0.28930.1504-0.16170.17760.02890.03060.1202-0.03420.285755.770917.063815.5059
245.9585-0.4445-1.51434.26490.07134.0068-0.1654-0.31980.14970.09950.09390.2560.13350.05420.12880.2123-0.0085-0.01290.0808-0.03620.240643.234314.687817.0777
259.29413.70722.94225.57643.06334.7138-0.05650.34840.4577-0.4550.0661-0.1622-0.61070.18560.01510.1713-0.01530.05160.17670.00330.242850.020928.11311.2296
263.29871.9173-1.46351.6379-0.00272.9925-0.05940.1856-0.0883-0.12860.1192-0.09850.21650.0112-0.01530.20130.01660.03540.1413-0.03340.207846.133218.41946.6703
272.38761.6483-3.39914.7104-1.51916.18950.00210.3754-0.0138-0.42240.1007-0.15250.10970.417-0.03690.1510.0045-0.03230.2588-0.02640.214555.072723.09078.9092
287.1592-0.451-0.66080.76410.1102-0.0199-0.1755-0.0647-0.1166-0.02590.1032-0.0970.11070.02910.10030.23290.01240.01690.1729-0.00780.175957.112822.001516.8893
294.3373-3.2165-2.44156.23696.33688.8251-0.0715-0.16840.376-0.54360.2679-0.3624-0.76920.4491-0.24750.1855-0.04770.02750.20610.02380.167184.330943.717228.2132
301.10871.8361.96454.9995.57647.69050.1724-0.1799-0.0287-0.016-0.34320.19370.1201-0.16880.14050.12510.00670.05160.17970.01240.258777.749330.118726.2911
316.24091.71184.83274.16880.71763.9264-0.0778-0.2092-0.34190.4587-0.0796-0.0151-0.08620.7466-0.04480.23390.05220.06140.3410.10960.340576.969332.460135.5314
323.12122.1572.3664.52333.31034.00830.1205-0.1221-0.20660.2631-0.0333-0.25240.06740.1732-0.04810.1868-0.00980.04430.19880.08750.181181.188232.500327.9108
332.96470.66360.55772.99870.59351.6132-0.069-0.21990.449-0.283-0.12940.530.0103-0.2320.18120.6961-0.075-0.36170.82810.02820.7692119.887515.3237102.1619
343.4212-0.72072.98842.0851-0.34515.3922-0.031-0.40480.0070.53520.0152-0.3611-0.01580.0790.03170.2723-0.0346-0.04110.35820.01240.365698.370.706873.4965
352.1854-0.73111.87037.4539-1.10133.576-0.00940.15170.02960.3563-0.0265-0.72270.12660.32120.0640.33690.0924-0.1320.4441-0.07560.4213-7.370437.3828-34.5055
362.5686-0.60852.23891.76740.18024.54070.24860.2897-0.2522-0.6803-0.1890.5310.1065-0.1225-0.06760.38370.0756-0.14360.34-0.03060.392113.593722.0792-5.4325
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'H' and (resid 2 through 25 )H2 - 25
2X-RAY DIFFRACTION2chain 'H' and (resid 26 through 52 )H26 - 52
3X-RAY DIFFRACTION3chain 'H' and (resid 53 through 68 )H53 - 68
4X-RAY DIFFRACTION4chain 'H' and (resid 69 through 114 )H69 - 114
5X-RAY DIFFRACTION5chain 'H' and (resid 115 through 178 )H115 - 178
6X-RAY DIFFRACTION6chain 'H' and (resid 179 through 197 )H179 - 197
7X-RAY DIFFRACTION7chain 'H' and (resid 198 through 220 )H198 - 220
8X-RAY DIFFRACTION8chain 'L' and (resid 1 through 18 )L1 - 18
9X-RAY DIFFRACTION9chain 'L' and (resid 19 through 103 )L19 - 103
10X-RAY DIFFRACTION10chain 'L' and (resid 104 through 114 )L104 - 114
11X-RAY DIFFRACTION11chain 'L' and (resid 115 through 151 )L115 - 151
12X-RAY DIFFRACTION12chain 'L' and (resid 152 through 164 )L152 - 164
13X-RAY DIFFRACTION13chain 'L' and (resid 165 through 199 )L165 - 199
14X-RAY DIFFRACTION14chain 'L' and (resid 200 through 214 )L200 - 214
15X-RAY DIFFRACTION15chain 'A' and (resid 2 through 12 )A2 - 12
16X-RAY DIFFRACTION16chain 'A' and (resid 13 through 52 )A13 - 52
17X-RAY DIFFRACTION17chain 'A' and (resid 53 through 68 )A53 - 68
18X-RAY DIFFRACTION18chain 'A' and (resid 69 through 114 )A69 - 114
19X-RAY DIFFRACTION19chain 'A' and (resid 115 through 138 )A115 - 138
20X-RAY DIFFRACTION20chain 'A' and (resid 139 through 178 )A139 - 178
21X-RAY DIFFRACTION21chain 'A' and (resid 179 through 197 )A179 - 197
22X-RAY DIFFRACTION22chain 'A' and (resid 198 through 216 )A198 - 216
23X-RAY DIFFRACTION23chain 'B' and (resid 1 through 18 )B1 - 18
24X-RAY DIFFRACTION24chain 'B' and (resid 19 through 33 )B19 - 33
25X-RAY DIFFRACTION25chain 'B' and (resid 34 through 49 )B34 - 49
26X-RAY DIFFRACTION26chain 'B' and (resid 50 through 76 )B50 - 76
27X-RAY DIFFRACTION27chain 'B' and (resid 77 through 91 )B77 - 91
28X-RAY DIFFRACTION28chain 'B' and (resid 92 through 114 )B92 - 114
29X-RAY DIFFRACTION29chain 'B' and (resid 115 through 129 )B115 - 129
30X-RAY DIFFRACTION30chain 'B' and (resid 130 through 151 )B130 - 151
31X-RAY DIFFRACTION31chain 'B' and (resid 152 through 164 )B152 - 164
32X-RAY DIFFRACTION32chain 'B' and (resid 165 through 213 )B165 - 213
33X-RAY DIFFRACTION33chain 'E' and (resid 24 through 127 )E24 - 127
34X-RAY DIFFRACTION34chain 'E' and (resid 128 through 273 )E128 - 273
35X-RAY DIFFRACTION35chain 'C' and (resid 24 through 116 )C24 - 116
36X-RAY DIFFRACTION36chain 'C' and (resid 117 through 273 )C117 - 273

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