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- PDB-7skj: Crystal structure of chloroplast triosephosphate isomerase from C... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7skj | ||||||
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Title | Crystal structure of chloroplast triosephosphate isomerase from Cuscuta australis | ||||||
![]() | Triosephosphate isomerase | ||||||
![]() | ISOMERASE / TIM / triosephosphate isomerase / glycolysis / gluconeogenesis / Calvin-Benson-Bassham Cycle | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Jones, G. / Vickers, C. / Patrick, W. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Crystal structure of chloroplast triosephosphate isomerase from Cuscuta australis Authors: Jones, G. / Vickers, C. / Patrick, W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 256.7 KB | Display | ![]() |
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PDB format | ![]() | 162.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 44.1 KB | Display | |
Data in CIF | ![]() | 65.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ohqS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 29568.379 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.01 Å3/Da / Density % sol: 38.7 % |
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Crystal grow | Temperature: 291.15 K / Method: vapor diffusion, sitting drop Details: 0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5, 25% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Aug 5, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9464 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→44.97 Å / Num. obs: 75939 / % possible obs: 99.8 % / Redundancy: 13.6 % / Biso Wilson estimate: 24.25 Å2 / CC1/2: 0.999 / Net I/σ(I): 16.3 |
Reflection shell | Resolution: 1.9→1.94 Å / Num. unique obs: 4271 / CC1/2: 0.928 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4OHQ Resolution: 1.9→44.97 Å / SU ML: 0.2141 / Cross valid method: FREE R-VALUE / Phase error: 22.4965 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.53 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→44.97 Å
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Refine LS restraints |
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LS refinement shell |
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