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Open data
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Basic information
Entry | Database: PDB / ID: 7sbe | ||||||
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Title | Structure of the K. lactis telomerase RNA binding domain | ||||||
![]() | Telomerase reverse transcriptase | ||||||
![]() | RNA BINDING PROTEIN / Telomerase | ||||||
Function / homology | ![]() telomerase catalytic core complex / : / telomeric DNA binding / telomere maintenance via telomerase / RNA-directed DNA polymerase / chromosome, telomeric region / nucleolus / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Skordalakes, E. / Tzfati, Y. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the K. lactis telomerase RNA binding domain Authors: Skordalakes, E. / Tzfati, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 106.3 KB | Display | ![]() |
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PDB format | ![]() | 80.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 436.2 KB | Display | ![]() |
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Full document | ![]() | 448.7 KB | Display | |
Data in XML | ![]() | 18.6 KB | Display | |
Data in CIF | ![]() | 23.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2r4gS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 27902.664 Da / Num. of mol.: 2 / Fragment: RNA binding domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.49 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / Details: 14% PEG3350 and 200 mM Na.citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 10, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 2.65→20 Å / Num. obs: 15880 / % possible obs: 99.3 % / Redundancy: 5.4 % / CC1/2: 0.99 / Net I/σ(I): 5.4 |
Reflection shell | Resolution: 2.65→2.72 Å / Num. unique obs: 1177 / CC1/2: 0.58 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 2R4G Resolution: 2.65→19.71 Å / SU ML: 0.47 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 38.12 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 183.96 Å2 / Biso mean: 91.4427 Å2 / Biso min: 41 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.65→19.71 Å
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 6
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