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- PDB-7s5j: Solution NMR structure of substrate bound peptidase domain from PCAT1 -
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Open data
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Basic information
Entry | Database: PDB / ID: 7s5j | ||||||
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Title | Solution NMR structure of substrate bound peptidase domain from PCAT1 | ||||||
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![]() | PEPTIDE BINDING PROTEIN / C39 peptidase domain CtA substrate | ||||||
Function / homology | ![]() ABC-type bacteriocin transporter activity / ATPase-coupled lipid transmembrane transporter activity / cysteine-type peptidase activity / ATP hydrolysis activity / proteolysis / ATP binding / membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing | ||||||
![]() | Bhattacharya, S. / Palillo, A. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural and dynamic studies of the peptidase domain from Clostridium thermocellum PCAT1. Authors: Bhattacharya, S. / Palillo, A. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1 MB | Display | ![]() |
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PDB format | ![]() | 889.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 408 KB | Display | ![]() |
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Full document | ![]() | 639.3 KB | Display | |
Data in XML | ![]() | 66 KB | Display | |
Data in CIF | ![]() | 86.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7n87C C: citing same article ( |
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Similar structure data | |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 16635.389 Da / Num. of mol.: 1 / Fragment: N-terminal residues, 1-148 / Mutation: C24A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372 Gene: Cthe_0534 / Plasmid: PMCSG20 / Production host: ![]() ![]() |
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#2: Protein/peptide | Mass: 2672.960 Da / Num. of mol.: 1 / Fragment: sequence database residues 7-30 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372 Gene: Cthe_0535 / Plasmid: pMCSG7 / Production host: ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
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Sample |
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Sample conditions | Ionic strength: 150 mM NaCl mM / Label: conditions_1 / pH: 7.0 / Pressure: 1 atm / Temperature: 288 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing / Software ordinal: 7 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 500 / Conformers submitted total number: 20 |