- PDB-7s44: Crystal structure of an N-acetyltransferase, C80T mutant, from He... -
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Open data
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Basic information
Entry
Database: PDB / ID: 7s44
Title
Crystal structure of an N-acetyltransferase, C80T mutant, from Helicobacter pullorum in the presence of Coenzyme A and dTDP-3-amino-3,6-dideoxy-D-galactose
Components
N-acetyltransferase
Keywords
TRANSFERASE
Function / homology
OXIDIZED COENZYME A / Chem-T3F
Function and homology information
Biological species
Helicobacter pullorum (bacteria)
Method
X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.4 Å
Mass: 18.015 Da / Num. of mol.: 220 / Source method: isolated from a natural source / Formula: H2O
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Details
Has ligand of interest
Y
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.56 Å3/Da / Density % sol: 51.99 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 11-16% PEG-3350, 200 mM KCl, 5 mM dTDP-3-amino-3,6-dideoxy-D-galactose, 5 mM coenzyme A, 100 mM HEPPS
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Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source
Source: SEALED TUBE / Type: BRUKER D8 QUEST / Wavelength: 1.5418 Å
Detector
Type: Bruker PHOTON II / Detector: PIXEL / Date: Nov 10, 2020
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
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