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- PDB-7s07: Crystal structure of Epstein-Barr virus glycoprotein gH/gL/gp42-p... -

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Basic information

Entry
Database: PDB / ID: 7s07
TitleCrystal structure of Epstein-Barr virus glycoprotein gH/gL/gp42-peptide in complex with human neutralizing antibodies 769B10 and 769C2
Components
  • (Envelope glycoprotein ...) x 2
  • 769B10 Fab heavy chain
  • 769B10 Fab light chain
  • 769C2 Fab Heavy chain
  • 769C2 Fab Light chain
  • Soluble gp42
KeywordsIMMUNE SYSTEM/VIRAL PROTEIN / Antibody / Fab / IMMUNE SYSTEM / gH/gL / Epstein-Barr virus / herpesvirus / IMMUNE SYSTEM-VIRAL PROTEIN complex
Function / homology
Function and homology information


host cell endosome membrane / host cell Golgi apparatus / carbohydrate binding / symbiont entry into host cell / fusion of virus membrane with host plasma membrane / viral envelope / host cell plasma membrane / virion membrane / membrane
Similarity search - Function
Herpesvirus glycoprotein L, rhadinovirus-type / Herpesvirus glycoprotein L, rhadinovirus-type superfamily / Viral glycoprotein L / Envelope glycoprotein L / Herpesvirus glycoprotein H main domain / Herpesvirus glycoprotein H / Herpesvirus glycoprotein H, C-terminal / Herpesvirus glycoprotein H, C-terminal domain superfamily / Herpesvirus glycoprotein H C-terminal domain / C-type lectin-like/link domain superfamily / C-type lectin fold
Similarity search - Domain/homology
Envelope glycoprotein L / Envelope glycoprotein H / Glycoprotein 42
Similarity search - Component
Biological speciesHuman gammaherpesvirus 4 (Epstein-Barr virus)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.29 Å
AuthorsChen, W.-H. / Kanekiyo, M. / Cohen, J.I. / Joyce, M.G.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)1ZIAAI000978-13 United States
CitationJournal: Immunity / Year: 2022
Title: Epstein-Barr virus gH/gL has multiple sites of vulnerability for virus neutralization and fusion inhibition.
Authors: Chen, W.H. / Kim, J. / Bu, W. / Board, N.L. / Tsybovsky, Y. / Wang, Y. / Hostal, A. / Andrews, S.F. / Gillespie, R.A. / Choe, M. / Stephens, T. / Yang, E.S. / Pegu, A. / Peterson, C.E. / ...Authors: Chen, W.H. / Kim, J. / Bu, W. / Board, N.L. / Tsybovsky, Y. / Wang, Y. / Hostal, A. / Andrews, S.F. / Gillespie, R.A. / Choe, M. / Stephens, T. / Yang, E.S. / Pegu, A. / Peterson, C.E. / Fisher, B.E. / Mascola, J.R. / Pittaluga, S. / McDermott, A.B. / Kanekiyo, M. / Joyce, M.G. / Cohen, J.I.
History
DepositionAug 30, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 9, 2022Provider: repository / Type: Initial release
Revision 1.1Nov 16, 2022Group: Database references / Category: citation
Item: _citation.journal_volume / _citation.page_first / _citation.page_last
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Envelope glycoprotein H
B: Envelope glycoprotein L
C: Soluble gp42
X: 769C2 Fab Heavy chain
Y: 769C2 Fab Light chain
H: 769B10 Fab heavy chain
L: 769B10 Fab light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)183,37511
Polymers182,4907
Non-polymers8854
Water0
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration, Purification by Gel-filtration column S200
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)90.695, 130.368, 93.612
Angle α, β, γ (deg.)90.000, 104.590, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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Envelope glycoprotein ... , 2 types, 2 molecules AB

#1: Protein Envelope glycoprotein H / gH


Mass: 72758.508 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human gammaherpesvirus 4 (Epstein-Barr virus)
Gene: gH, BXLF2 / Production host: Homo sapiens (human) / References: UniProt: P03231
#2: Protein Envelope glycoprotein L / gL


Mass: 10626.060 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human gammaherpesvirus 4 (Epstein-Barr virus)
Gene: gL, BKRF2 / Production host: Homo sapiens (human) / References: UniProt: P03212

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Antibody , 4 types, 4 molecules XYHL

#4: Antibody 769C2 Fab Heavy chain


Mass: 23840.705 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#5: Antibody 769C2 Fab Light chain


Mass: 22782.020 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#6: Antibody 769B10 Fab heavy chain


Mass: 25264.258 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#7: Antibody 769B10 Fab light chain


Mass: 23452.006 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)

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Protein/peptide / Sugars , 2 types, 5 molecules C

#3: Protein/peptide Soluble gp42


Mass: 3766.208 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human gammaherpesvirus 4 (Epstein-Barr virus)
Gene: BZLF2 / Production host: Homo sapiens (human) / References: UniProt: P0C6Z5
#8: Sugar
ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE / N-Acetylglucosamine


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.98 Å3/Da / Density % sol: 58.68 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop
Details: 0.1M Bis-Tris pH6.5, 20% PEG3350, 7.5% Glycerol, 0.7% Dioxane

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.987 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Jun 20, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.987 Å / Relative weight: 1
ReflectionResolution: 3.29→50 Å / Num. obs: 41284 / % possible obs: 75.6 % / Redundancy: 4.5 % / CC1/2: 0.65 / Net I/σ(I): 5.5
Reflection shellResolution: 3.29→3.39 Å / Num. unique obs: 2941 / CC1/2: 0.35

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Processing

Software
NameVersionClassification
PHENIX1.17.1_3660refinement
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5W0K

5w0k


Resolution: 3.29→48.58 Å / SU ML: 0.44 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 29.5 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2909 3045 8.86 %
Rwork0.243 31311 -
obs0.2473 34356 54.66 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 150.16 Å2 / Biso mean: 50.5178 Å2 / Biso min: 14.54 Å2
Refinement stepCycle: final / Resolution: 3.29→48.58 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12637 0 56 0 12693
Biso mean--39.19 --
Num. residues----1651
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 22

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
3.29-3.340.795750.413648532
3.35-3.40.523160.36271071134
3.4-3.460.3258280.33341852137
3.46-3.520.4076240.33733636013
3.52-3.590.3286510.318845050118
3.59-3.660.3339580.339464470225
3.66-3.740.3588700.307778485429
3.74-3.830.3309930.281687096335
3.83-3.920.3153960.27991038113439
3.92-4.030.30291080.27591094120243
4.03-4.150.29961270.24721355148251
4.15-4.280.29761580.23391545170360
4.28-4.430.27931720.21971729190166
4.44-4.610.27361770.20981858203572
4.61-4.820.28381970.21372112230980
4.82-5.080.25352180.21192197241584
5.08-5.390.2992250.24262315254089
5.39-5.810.31852450.2632440268595
5.81-6.390.31042440.28432535277997
6.39-7.310.35322460.28572571281799
7.32-9.20.24662470.22192563281098
9.21-48.580.24912500.21152535278597
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.5242-0.20340.56240.38880.18741.00070.02980.2526-0.2293-0.00160.0859-0.17550.26130.1928-0.11060.27740.01770.00560.302-0.02950.187123.769-3.40445.279
21.69270.39231.4058-0.07620.72310.51930.0075-0.03390.0068-0.02910.07810.09440.0594-0.0285-0.05570.1826-0.02750.06110.3186-0.02050.2108-2.209-1.35638.002
32.7341-0.25141.2852.642-0.04553.7378-0.1588-0.11810.8314-0.13870.02350.1587-0.4622-0.00290.06090.28220.0266-0.00580.2647-0.01240.5143-28.96910.87928.18
40.2083-0.6809-0.97742.12383.02264.2775-0.1203-0.15750.3507-0.31770.5801-0.2717-0.23510.0731-0.4330.36710.10180.3260.9791-0.15710.928452.8646.00850.253
53.64580.98081.28333.7075-1.86423.0893-0.1985-0.05540.10550.5833-0.2828-0.21510.42850.79480.43070.18710.06170.02580.4839-0.14890.526148.081-3.20758.111
69.9319-8.3224-1.76737.57182.83063.27290.56330.6031.0236-0.4699-0.0297-2.10430.06561.5331-0.43820.44920.4325-0.14310.93960.20961.116361.607-7.66250.02
71.2939-0.6678-0.24635.23371.56642.4197-0.396-0.026-0.09150.67870.49670.1250.24570.14770.0030.18060.036-0.06620.4389-0.00450.142537.246-5.81956.618
82.00054.33977.02191.85973.01044.87720.7272-0.4978-0.80550.2332-0.2285-0.08750.8173-0.3601-0.4610.44650.2198-0.06350.6074-0.1080.2994-31.265-9.83122.378
96.76495.92014.30799.8167-2.72342.0025-0.14480.7310.2869-0.45930.2969-0.6385-0.05150.0248-0.14340.1519-0.02330.01190.3714-0.12150.7198-18.304-9.87917.884
100.05460.3474-0.1173.48991.23573.22460.0195-0.61520.05150.08440.03190.11780.1577-0.2937-0.0390.30620.2328-0.35260.5657-0.17250.0261-4.349-16.12518.191
119.2336-9.85132.59652.00081.20949.9246-0.3373-1.3381-1.13880.98260.03820.76380.5318-0.36580.26560.57-0.11410.40420.9431-0.35020.98538.171-19.18520.439
120.1519-0.40350.00051.0544-0.0018-0.00050.29881.9208-0.0373-0.8794-0.36120.36590.011-1.35640.04251.0543-0.13410.06110.74690.04460.280513.876-12.01723.546
132.79271.22850.1734.7235-2.08771.1358-0.1764-0.06490.4677-0.16510.00040.1055-0.24740.09930.21590.5971-0.3674-0.08650.58070.12450.891421.85-2.69729.263
140.9643-0.9257-0.46110.91150.01553.75930.4283-0.1690.2301-0.2584-0.39830.5334-0.4337-0.6128-0.0680.38370.09850.10320.81410.00950.478749.255-24.65890.173
153.5215-1.83510.42083.8865-0.22424.0687-0.14-0.4267-0.19790.36270.28750.60010.1424-0.404-0.16990.20640.0554-0.06580.50740.01720.537645.308-21.87281.166
161.2305-1.04071.85521.126-1.7563.41920.2044-0.0671-0.40410.03770.11080.56070.1107-0.2085-0.21560.36110.0499-0.03980.5019-0.23030.642358.188-31.09791.342
172.5855-0.31051.40273.56350.45923.80080.24170.01350.005-0.19080.07290.17250.34830.6649-0.46190.42640.219-0.08270.5662-0.12380.733969.278-34.72199.43
186.0381-3.9372-1.99413.942-0.75253.71250.36220.46070.63030.00390.28970.44310.0496-0.627-0.47780.45860.60440.20370.5973-0.25210.919362.56-30.586106.763
191.8853-1.2249-0.21740.79620.14170.02380.22520.63670.57470.90870.1806-0.82190.27160.5435-0.3940.97350.1791-0.24980.9892-0.07150.78877.991-38.177113.512
205.1322-0.11791.62440.1462-0.00330.4877-0.27070.0459-0.4305-0.2957-0.2037-0.71550.22030.41120.06380.78990.45840.40540.4726-0.4030.600265.701-35.54865.673
212.2292-1.14360.16534.1561-2.45131.6153-0.02460.3158-0.4292-0.27250.2124-0.1894-0.08130.5525-0.32410.38350.22570.01460.6935-0.12860.368860.471-23.99366.676
220.47240.2147-0.92742.6827-0.77031.87140.31570.2313-0.8338-0.525-0.2065-0.00610.09270.8899-0.1410.59210.3603-0.14851.0437-0.46880.60369.243-27.11165.501
230.72440.0197-0.09331.0735-0.17820.18510.15120.211-0.61830.2348-0.00960.04720.3168-0.255-0.05220.50710.4508-0.1710.1455-0.30071.096467.239-40.45386.674
245.5237-0.1742-0.04963.0967-0.68425.21820.8211-0.1183-1.4831-0.76-1.20230.55660.33980.06110.2960.57960.1175-0.11570.6148-0.17170.931968.888-50.78191.698
251.9774-1.6171-0.49851.86692.02584.7940.2215-0.0256-0.2819-0.4295-0.70040.30380.1995-0.44930.5420.61650.4085-0.17610.3891-0.13650.816469.646-45.1993.355
260.1102-0.5043-0.64752.35232.98713.7725-1.03150.07750.48530.1726-0.51490.10041.82260.411.44340.94780.0743-0.0440.66520.01291.026673.875-57.036101.697
272.2981-0.0838-4.07561.1295-2.582.001-0.4381-0.8404-0.13260.0718-0.1035-0.27360.24051.15010.55580.95590.3496-0.26951.01090.46491.651780.535-52.23796.846
286.91540.5249-0.11740.22310.26260.3620.1507-0.3626-0.0790.2557-0.18890.17180.4747-0.186-0.03791.16930.1635-0.38780.4452-0.11710.089128.13318.1387.901
292.6950.97650.51532.53060.87084.0704-0.1930.371-0.23630.56680.14030.2090.60570.13230.06820.59580.00950.00340.1626-0.01160.257125.28915.13575.321
306.6643-0.8831-2.01342.7132-1.13065.7871-0.0788-0.0456-0.86250.2498-0.2472-0.83790.67350.46150.24080.7484-0.009-0.20370.38780.09170.207729.15915.70582.698
310.73940.56430.90371.06510.97411.7601-0.70550.17930.34890.91410.0721-0.5272-0.4740.09490.14650.85030.0266-0.14550.27120.2714-0.264329.00133.41783.653
327.07971.2786-2.66081.9367-2.76164.83710.3538-1.0878-0.28950.4290.1314-0.0452-0.3064-0.2011-0.53030.70360.0387-0.23890.6130.05690.67235.72640.24598.279
331.90812.14270.78123.2942-1.22665.30880.0616-0.690.570.86010.43930.8759-0.3209-0.6225-0.13450.68960.203-0.06450.6593-0.12750.253733.31146.30592.756
341.6944-0.0067-2.13450.17430.11543.8044-0.3983-0.38390.55980.24250.18490.4757-1.1530.26720.20450.5381-0.034-0.87640.55080.11710.35738.21844.93892.837
353.142-0.4492-5.18480.06390.73688.5117-0.0028-0.71520.5234-0.0347-0.1782-0.2455-0.57231.21130.07411.7922-0.02760.32610.9651-0.23690.782934.3759.468104.001
362.8495-0.092-0.93181.21950.96083.54260.5561-0.12640.10960.9193-0.05620.11590.09810.0392-0.39811.92120.3246-0.19520.8314-0.11880.38838.54743.119104.797
371.9999222.00012.00021.99980.5303-0.13990.9860.56021.1023-1.2361-1.13080.8996-1.62881.54560.09760.24210.7552-0.06610.72348.97660.27499.118
383.611-3.2487-3.64536.04624.46354.1859-0.10280.2108-0.1116-0.08720.2809-0.65150.0060.3133-0.16180.15780.3695-0.08140.5648-0.04410.389922.43640.27167.22
391.8585-0.59260.07282.61221.81941.39570.10160.24120.0095-0.6188-0.0567-0.3496-0.39020.3024-0.12010.10030.02680.10220.51690.08420.455920.16831.25163.383
405.39871.41520.50074.97093.70845.14680.1412-0.24830.22590.4566-0.38640.35340.1299-0.21650.23130.15120.1440.13480.79-0.090.204412.61129.64272.91
410.6682-0.7959-0.41992.64661.53940.89860.12560.2457-0.3483-0.0686-0.34420.7344-0.1011-0.27090.2950.3021-0.0796-0.1080.3025-0.0180.366412.45338.63568.262
423.17061.09960.65951.49981.45033.8127-0.11530.3883-0.43460.15870.1043-0.16610.20120.2387-0.1256-0.16740.0799-0.08580.40790.20190.23724.15227.16767.375
430.69-0.441-0.37580.26810.24590.1903-0.2817-0.2150.19770.2966-0.0125-0.1021-0.17460.02390.24180.44790.0368-0.07770.1653-0.00590.605124.91554.36678.104
445.7659-3.52044.94364.0099-5.54327.60.2705-0.128-0.40150.62640.6267-0.62580.02390.1582-0.47880.73130.0234-0.43240.6134-0.20750.670949.48351.7395.835
456.2638-1.4510.41613.93930.46354.68730.31770.754-0.16440.082-0.0742-0.3073-0.37380.6564-0.24530.39850.0831-0.01780.3664-0.01290.21739.4553.21680.448
469.9955-2.26271.00023.2622-0.14742.5750.22450.2718-0.14030.35350.26310.5081-0.2846-0.1565-0.28920.59790.11710.10280.462-0.07060.301828.87548.74384.144
478.93810.45093.88141.22310.93862.151-0.58860.56960.85950.56750.5447-0.2804-0.32880.4850.04110.87640.1583-0.32450.4350.07160.724350.93352.00586.48
486.4839-0.86953.71280.1154-0.48092.0769-0.3270.00590.57360.1905-0.1752-0.423-0.40380.26850.47430.80280.0104-0.20930.58940.17651.013744.12961.35482.104
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 18:216 )A18 - 216
2X-RAY DIFFRACTION2( CHAIN A AND RESID 217:453 )A217 - 453
3X-RAY DIFFRACTION3( CHAIN A AND RESID 454:674 )A454 - 674
4X-RAY DIFFRACTION4( CHAIN B AND RESID 27:41 )B27 - 41
5X-RAY DIFFRACTION5( CHAIN B AND RESID 42:64 )B42 - 64
6X-RAY DIFFRACTION6( CHAIN B AND RESID 65:77 )B65 - 77
7X-RAY DIFFRACTION7( CHAIN B AND RESID 78:124 )B78 - 124
8X-RAY DIFFRACTION8( CHAIN C AND RESID 47:51 )C47 - 51
9X-RAY DIFFRACTION9( CHAIN C AND RESID 52:56 )C52 - 56
10X-RAY DIFFRACTION10( CHAIN C AND RESID 57:61 )C57 - 61
11X-RAY DIFFRACTION11( CHAIN C AND RESID 62:66 )C62 - 66
12X-RAY DIFFRACTION12( CHAIN C AND RESID 67:71 )C67 - 71
13X-RAY DIFFRACTION13( CHAIN C AND RESID 72:79 )C72 - 79
14X-RAY DIFFRACTION14( CHAIN X AND RESID 1:17 )X1 - 17
15X-RAY DIFFRACTION15( CHAIN X AND RESID 18:92 )X18 - 92
16X-RAY DIFFRACTION16( CHAIN X AND RESID 93:146 )X93 - 146
17X-RAY DIFFRACTION17( CHAIN X AND RESID 147:188 )X147 - 188
18X-RAY DIFFRACTION18( CHAIN X AND RESID 189:207 )X189 - 207
19X-RAY DIFFRACTION19( CHAIN X AND RESID 208:212 )X208 - 212
20X-RAY DIFFRACTION20( CHAIN Y AND RESID 2:18 )Y2 - 18
21X-RAY DIFFRACTION21( CHAIN Y AND RESID 19:56 )Y19 - 56
22X-RAY DIFFRACTION22( CHAIN Y AND RESID 57:86 )Y57 - 86
23X-RAY DIFFRACTION23( CHAIN Y AND RESID 87:148 )Y87 - 148
24X-RAY DIFFRACTION24( CHAIN Y AND RESID 149:166 )Y149 - 166
25X-RAY DIFFRACTION25( CHAIN Y AND RESID 167:180 )Y167 - 180
26X-RAY DIFFRACTION26( CHAIN Y AND RESID 181:195 )Y181 - 195
27X-RAY DIFFRACTION27( CHAIN Y AND RESID 196:207 )Y196 - 207
28X-RAY DIFFRACTION28( CHAIN H AND RESID 1:22 )H1 - 22
29X-RAY DIFFRACTION29( CHAIN H AND RESID 23:70 )H23 - 70
30X-RAY DIFFRACTION30( CHAIN H AND RESID 71:93 )H71 - 93
31X-RAY DIFFRACTION31( CHAIN H AND RESID 94:140 )H94 - 140
32X-RAY DIFFRACTION32( CHAIN H AND RESID 141:153 )H141 - 153
33X-RAY DIFFRACTION33( CHAIN H AND RESID 154:169 )H154 - 169
34X-RAY DIFFRACTION34( CHAIN H AND RESID 170:181 )H170 - 181
35X-RAY DIFFRACTION35( CHAIN H AND RESID 182:194 )H182 - 194
36X-RAY DIFFRACTION36( CHAIN H AND RESID 195:210 )H195 - 210
37X-RAY DIFFRACTION37( CHAIN H AND RESID 211:211 )H211
38X-RAY DIFFRACTION38( CHAIN L AND RESID 1:18 )L1 - 18
39X-RAY DIFFRACTION39( CHAIN L AND RESID 19:38 )L19 - 38
40X-RAY DIFFRACTION40( CHAIN L AND RESID 39:58 )L39 - 58
41X-RAY DIFFRACTION41( CHAIN L AND RESID 59:84 )L59 - 84
42X-RAY DIFFRACTION42( CHAIN L AND RESID 85:102 )L85 - 102
43X-RAY DIFFRACTION43( CHAIN L AND RESID 103:115 )L103 - 115
44X-RAY DIFFRACTION44( CHAIN L AND RESID 116:130 )L116 - 130
45X-RAY DIFFRACTION45( CHAIN L AND RESID 131:158 )L131 - 158
46X-RAY DIFFRACTION46( CHAIN L AND RESID 159:172 )L159 - 172
47X-RAY DIFFRACTION47( CHAIN L AND RESID 173:193 )L173 - 193
48X-RAY DIFFRACTION48( CHAIN L AND RESID 194:214 )L194 - 214

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