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- PDB-7ryn: CD1a-sulfatide-gdTCR complex -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 7ryn
TitleCD1a-sulfatide-gdTCR complex
Components
  • (T cell receptor ...) x 2
  • Beta-2-microglobulin
  • T-cell surface glycoprotein CD1a
KeywordsIMMUNE SYSTEM / CD1 / TCR / lipid antigen
Function / homology
Function and homology information


exogenous lipid antigen binding / antigen processing and presentation, endogenous lipid antigen via MHC class Ib / lipopeptide binding / endogenous lipid antigen binding / antigen processing and presentation, exogenous lipid antigen via MHC class Ib / alpha-beta T cell receptor complex / T cell receptor complex / immunoglobulin complex / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains ...exogenous lipid antigen binding / antigen processing and presentation, endogenous lipid antigen via MHC class Ib / lipopeptide binding / endogenous lipid antigen binding / antigen processing and presentation, exogenous lipid antigen via MHC class Ib / alpha-beta T cell receptor complex / T cell receptor complex / immunoglobulin complex / Translocation of ZAP-70 to Immunological synapse / Phosphorylation of CD3 and TCR zeta chains / alpha-beta T cell activation / Generation of second messenger molecules / Co-inhibition by PD-1 / negative regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / transferrin transport / cellular response to iron ion / Endosomal/Vacuolar pathway / response to bacterium / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide antigen assembly with MHC class II protein complex / cellular response to iron(III) ion / MHC class II protein complex / negative regulation of forebrain neuron differentiation / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / regulation of erythrocyte differentiation / HFE-transferrin receptor complex / response to molecule of bacterial origin / MHC class I peptide loading complex / T cell mediated cytotoxicity / positive regulation of T cell cytokine production / antigen processing and presentation of endogenous peptide antigen via MHC class I / antigen processing and presentation of exogenous peptide antigen via MHC class II / positive regulation of immune response / MHC class I protein complex / positive regulation of T cell activation / peptide antigen binding / positive regulation of receptor-mediated endocytosis / negative regulation of neurogenesis / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / multicellular organismal-level iron ion homeostasis / specific granule lumen / phagocytic vesicle membrane / recycling endosome membrane / Interferon gamma signaling / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / negative regulation of epithelial cell proliferation / MHC class II protein complex binding / Modulation by Mtb of host immune system / late endosome membrane / sensory perception of smell / positive regulation of cellular senescence / tertiary granule lumen / Downstream TCR signaling / DAP12 signaling / T cell differentiation in thymus / T cell receptor signaling pathway / negative regulation of neuron projection development / ER-Phagosome pathway / protein refolding / early endosome membrane / protein homotetramerization / adaptive immune response / amyloid fibril formation / intracellular iron ion homeostasis / learning or memory / endosome membrane / immune response / membrane raft / endoplasmic reticulum lumen / Amyloid fiber formation / Golgi membrane / lysosomal membrane / innate immune response / external side of plasma membrane / focal adhesion / Neutrophil degranulation / SARS-CoV-2 activates/modulates innate and adaptive immune responses / structural molecule activity / endoplasmic reticulum / Golgi apparatus / protein homodimerization activity / extracellular space / extracellular exosome / extracellular region / identical protein binding / membrane / plasma membrane / cytosol
Similarity search - Function
: / : / MHC-I family domain / T-cell receptor alpha chain, constant domain / Domain of unknown function (DUF1968) / : / Immunoglobulin V-Type / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like ...: / : / MHC-I family domain / T-cell receptor alpha chain, constant domain / Domain of unknown function (DUF1968) / : / Immunoglobulin V-Type / Beta-2-Microglobulin / : / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / Immunoglobulin V-set domain / MHC classes I/II-like antigen recognition protein / Immunoglobulin V-set domain / : / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
Chem-CIS / DI(HYDROXYETHYL)ETHER / T cell receptor gamma variable 4 / T cell receptor delta variable 1 / T cell receptor alpha chain constant / T cell receptor beta constant 1 / T-cell surface glycoprotein CD1a / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å
AuthorsWegrecki, M. / Le Nours, J. / Rossjohn, J.
Funding support Australia, 1items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia) Australia
CitationJournal: Nat Commun / Year: 2022
Title: Atypical sideways recognition of CD1a by autoreactive gamma delta T cell receptors.
Authors: Wegrecki, M. / Ocampo, T.A. / Gunasinghe, S.D. / von Borstel, A. / Tin, S.Y. / Reijneveld, J.F. / Cao, T.P. / Gully, B.S. / Le Nours, J. / Moody, D.B. / Van Rhijn, I. / Rossjohn, J.
History
DepositionAug 25, 2021Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 18, 2022Provider: repository / Type: Initial release
Revision 1.1Jul 13, 2022Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.3Oct 30, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: T-cell surface glycoprotein CD1a
B: Beta-2-microglobulin
C: T cell receptor gamma variable 4,T cell receptor beta constant 1
D: T cell receptor delta variable 1,T cell receptor alpha chain constant
hetero molecules


Theoretical massNumber of molelcules
Total (without water)98,58715
Polymers96,8054
Non-polymers1,78211
Water25214
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: surface plasmon resonance
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)234.633, 42.206, 123.065
Angle α, β, γ (deg.)90.000, 114.288, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z
Components on special symmetry positions
IDModelComponents
11C-301-

SO4

21C-301-

SO4

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Components

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Protein , 2 types, 2 molecules AB

#1: Protein T-cell surface glycoprotein CD1a / T-cell surface antigen T6/Leu-6 / hTa1 thymocyte antigen


Mass: 32593.562 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: CD1A / Production host: Homo sapiens (human) / References: UniProt: P06126
#2: Protein Beta-2-microglobulin


Mass: 12602.105 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Homo sapiens (human) / References: UniProt: P61769

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T cell receptor ... , 2 types, 2 molecules CD

#3: Protein T cell receptor gamma variable 4,T cell receptor beta constant 1


Mass: 27987.078 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRGV4, TCRGV4, TRBC1 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A0C4DH28, UniProt: P01850
#4: Protein T cell receptor delta variable 1,T cell receptor alpha chain constant


Mass: 23622.590 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TRDV1, TRAC, TCRA / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: A0A1B0GX56, UniProt: P01848

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Sugars , 1 types, 1 molecules

#7: Sugar ChemComp-NAG / 2-acetamido-2-deoxy-beta-D-glucopyranose / N-acetyl-beta-D-glucosamine / 2-acetamido-2-deoxy-beta-D-glucose / 2-acetamido-2-deoxy-D-glucose / 2-acetamido-2-deoxy-glucose / N-ACETYL-D-GLUCOSAMINE


Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Formula: C8H15NO6
IdentifierTypeProgram
DGlcpNAcbCONDENSED IUPAC CARBOHYDRATE SYMBOLGMML 1.0
N-acetyl-b-D-glucopyranosamineCOMMON NAMEGMML 1.0
b-D-GlcpNAcIUPAC CARBOHYDRATE SYMBOLPDB-CARE 1.0
GlcNAcSNFG CARBOHYDRATE SYMBOLGMML 1.0

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Non-polymers , 5 types, 24 molecules

#5: Chemical ChemComp-CIS / (15Z)-N-((1S,2R,3E)-2-HYDROXY-1-{[(3-O-SULFO-BETA-D-GALACTOPYRANOSYL)OXY]METHYL}HEPTADEC-3-ENYL)TETRACOS-15-ENAMIDE / (2S,3R,4E)-N-NERVONIC-1-[BETA-D-(3-SULFATE)-GALACTOPYRANOSYL]-2-AMINO-OCTADECENE-3-OL / CIS-TETRACOSENOYL SULFATIDE


Mass: 890.301 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C48H91NO11S / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C2H6O2
#8: Chemical ChemComp-PEG / DI(HYDROXYETHYL)ETHER


Mass: 106.120 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O3
#9: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
#10: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 14 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.95 Å3/Da / Density % sol: 58.3 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / Details: 15% PEG 4000, 0.15M Ammonium Sulfate, 0.1M MES pH6

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.95373 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 18, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.95373 Å / Relative weight: 1
ReflectionResolution: 2.7→42.93 Å / Num. obs: 31003 / % possible obs: 99.9 % / Redundancy: 11.4 % / Biso Wilson estimate: 60.41 Å2 / CC1/2: 0.991 / Net I/σ(I): 14.2
Reflection shellResolution: 2.7→2.93 Å / Num. unique obs: 4097 / CC1/2: 0.909

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7RYM

7rym


Resolution: 2.7→42.93 Å / SU ML: 0.3877 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 34.0219
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2683 1569 5.07 %
Rwork0.2321 29380 -
obs0.234 30949 99.9 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 99.53 Å2
Refinement stepCycle: LAST / Resolution: 2.7→42.93 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5961 0 116 14 6091
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00236234
X-RAY DIFFRACTIONf_angle_d0.568496
X-RAY DIFFRACTIONf_chiral_restr0.0424929
X-RAY DIFFRACTIONf_plane_restr0.00331081
X-RAY DIFFRACTIONf_dihedral_angle_d13.05372127
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.7-2.790.40411540.35592636X-RAY DIFFRACTION99.86
2.79-2.890.40341550.31742601X-RAY DIFFRACTION99.96
2.89-30.34811450.29172667X-RAY DIFFRACTION99.93
3-3.140.29981200.2982626X-RAY DIFFRACTION99.89
3.14-3.30.2821350.27692663X-RAY DIFFRACTION99.82
3.3-3.510.27811550.23262665X-RAY DIFFRACTION99.75
3.51-3.780.27931180.23072650X-RAY DIFFRACTION100
3.78-4.160.28311450.21522667X-RAY DIFFRACTION99.96
4.16-4.760.2181540.18042681X-RAY DIFFRACTION99.96
4.76-60.2461420.21182713X-RAY DIFFRACTION99.93
6-42.930.2391460.22452811X-RAY DIFFRACTION99.8
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.287439986311.42876242642-1.386768579922.10264686764-2.73968598048.35238438019-0.3465279732040.253082374026-0.06334846461930.04754373144490.2033132904620.126263378121-0.604663483773-0.8947902018980.2490069858250.5824806383260.11601511292-0.0141372368130.4567001574670.00620165033820.88263652761516.12718.281-3.882
23.443080242590.891317933132-0.2911443550164.73309000844-2.41572363676.21555801356-0.2321573632510.235710201443-0.864177126136-0.5192878524220.2806342088360.01075491674180.756173852662-0.636646459606-0.02429487854530.509729836163-0.0223458661228-0.01773532597740.434528157571-0.07541752762050.62601587006118.8473.882-5.303
34.854570834681.51325097023-0.4459785628623.197464797720.07632865150421.99700172823-0.0124800773515-0.0645823098368-0.183380249302-0.1496774586360.0592331634518-0.6305229289540.0942337082396-0.109442775627-0.01328480758560.617666411301-0.02180739410630.08214953975050.2417610516080.02115745748790.87045721371751.55328.455-1.414
44.51063261437-4.70134990345-3.829283288726.66795436380.937132567028.437822048590.461534298862-0.0177178539369-1.385991970750.281723847197-0.335553924982-0.006452607148991.08449308239-1.05419763025-0.1363084422710.666203963123-0.139937307012-0.1760388308560.5165635435120.1936385270931.0501636268933.1747.62412.835
50.996575213715-0.9309919989161.610443722894.72676816006-4.680040537435.167438111230.60499309012-0.158473097202-0.49195042838-1.67970390910.3680371478111.009533637120.9150052076490.220015985999-0.2661453777090.5086977569940.04130822621080.08666738355610.3804195975220.1292794713590.49338031334640.33424.2189.1
63.831251189-2.653224986321.963873923085.81314137173.588572967577.15096158473-0.20337091090.6035415138290.222059056319-0.145796087387-0.2328566668840.277716662258-0.1790501984210.5655721064630.47536166930.691227946063-0.04214811433930.0704181775540.2564912876790.06863297023980.85073038962443.14840.3396.979
71.431175168740.8579298841890.4000150700331.12474985627-0.2043046668991.423621878450.115574637084-0.2037433470020.436713632271-0.148527884169-0.2197609265922.09322956916-0.197635791816-0.5580001881350.07623202359180.3243204558510.0779217875091-0.09847900221490.4570488129510.0005970281708120.5257375894736.35129.3237.894
89.556858065873.983194570221.376085973913.90269694201-3.609710082968.588739893690.4545966468-0.76900231145-0.4162092936761.38888394005-0.200252809408-0.6946182191920.487526045313-0.316612873565-0.2752640073880.5378353496440.0041465330593-0.2284107790550.4875073923550.1350372623230.62436810071828.22214.64412.685
97.53604622023-1.30564102152-0.09134066014262.41820540407-3.162054982987.827513440940.604253941578-1.92022296946-0.8753701806411.595685292480.395846582398-0.1300919015820.2101609958570.465955312095-0.5893137144910.528301608403-0.00842480290641-0.03306436964840.53546422954-0.2033384189520.83242918733632.82731.4320.004
103.856231640744.435404368572.419883183596.529321107254.033107731334.071662959230.0967060379778-0.364026032977-0.2047178518290.307676797710.0468883656251-0.221622784599-0.0420820225145-0.268651522924-0.2146331507730.5687407082160.04981617950610.0307752464810.418540367130.1239283278510.54894869496729.79123.7716.706
115.03608654254.795377191143.567689958984.66326859853.569631286052.863407052151.1799049192-1.761172670321.208889061451.04139287586-0.988393170613-0.2273552558470.3716753867821.369567386780.03857641611760.890949565675-0.0789185657156-0.1136670761690.724040553607-0.1507631942620.89151381392343.58840.04919.414
128.792295532265.8366915867-0.7365039094924.406195081960.5495834874031.958618748560.328205566297-1.91954360219-1.285982157541.07693893530.0194816490371-0.0912704635212-0.2978733994570.496350066861-0.197229635440.7797820059720.0333529722138-0.02859869085320.4811266337050.231803041320.6756963640634.38919.6219.826
139.99467613636.32034161612-1.073930759864.10120660628-0.09714543178592.70712744414-0.213465657877-0.3713427171481.000993449050.202111656289-0.176730490699-1.76789277945-0.813550136024-0.3572116745720.6047577293690.647899470137-0.0301288711095-0.1268428803550.5554128590770.05420933255531.1541995831644.34428.78415.918
144.673913768133.162324772552.131085580117.866393243091.564472710766.03459712926-0.0182331471489-0.1294629079270.0775892390751-0.297832164178-0.05406468720830.8179426371660.0206260422082-0.1078571644410.08679390905220.6347207116310.04162041715060.09862842141560.6403351592850.06529470033180.645573880037-1.6343.73824.592
152.434319821890.377601345425-0.5534365218880.7845975817810.1835456353444.997141874140.477974643034-0.789791869588-0.1304321823091.37411948489-0.2045835104360.187215258786-0.0229532724706-0.476439022515-0.1597169184261.00178935144-0.004210580403170.2923501147370.9045625103830.1741170938940.900709990482-5.0594.09743.085
162.77854411161-1.07402633949-0.8806460875761.954491324940.7624348114015.32159895244-0.126224322869-0.5322575360510.08420082896930.7578436440020.2043176673860.511905442208-0.4712670522940.71363212425-0.09749554711611.50684448007-0.1988919829180.52625478471.550805348430.227760737221.14869909441-14.7831.05853.533
178.51734406947-4.14515416009-8.829202284449.033725236174.981107748699.194385862570.027591031136-0.198923864955-0.07064365397010.6805473870480.369243938301-1.462810272580.70723932526-0.383574142816-0.0008910934184361.15340387128-0.0514718748395-0.1815254799032.114231386890.3962737795721.0631572678416.8164.94643.639
18-0.0529128313474-0.0388635986713-0.01768831596052.9136455236-1.151431909270.4481755315040.242637656669-1.190565238520.2476862765920.0918936483789-0.263067200615-0.8837745112150.9455381144550.967668555719-0.1777815615641.04994333079-0.159772305176-0.08506842801332.123559632790.5358106185431.0915968100321.2911.4638.678
193.72115978439-2.10141996283-4.297237794896.73203885996-1.744417852488.047132562450.63770598897-2.219224760060.6655758649790.466588937559-0.954243802953-0.0384698173674-0.445544818371.029420117780.3521251633150.879607288513-0.3555198493420.2256314948611.56415006267-0.2647008605950.95499612066210.28814.66637.104
204.413691245033.24679882972-6.11098136686.93893068738-2.318298560359.479886845370.894491983935-2.24525618431-0.9220834177391.21383364331-0.808070400101-1.59913133271-0.7496045032131.326874275060.02852178157270.926445485514-0.353069022722-0.1135076928161.811230711630.118802440060.91213769030122.17418.13240.263
211.421595795241.68092383887-1.400932458891.83236416999-2.134563238534.188411840420.965243501705-2.58614969320.8772674997580.829182005127-1.103286148380.373768911177-0.0971823057050.9779649371620.07827445376110.935840097102-0.358611302808-0.001844789109781.82907851146-0.02232344585540.82312463368412.44612.06437.034
221.50030757072-1.46146692065-2.425354895343.133382911690.3755318760795.422595787790.181859296226-1.030850035220.2187340601860.2734268006280.1143184125690.3009177185050.568956829588-0.174302887435-0.2488674264421.27666823392-0.2795319010460.1681786285392.43209014560.1650606318741.17600970789-1.7770.38165.175
231.6373207303-2.633687272612.199926770878.13556208575-1.703384827335.243439972110.3834413620010.5736979104552.258802331140.5558548865480.112200996250.588681201164-1.678113036511.029295261-1.03970846622.27867231615-0.4121195018870.2335725294261.794345622660.2947681091041.47881117182-2.7094.7161.365
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 7:81 )A7 - 81
2X-RAY DIFFRACTION2( CHAIN A AND RESID 82:176 )A82 - 176
3X-RAY DIFFRACTION3( CHAIN A AND RESID 177:280 )A177 - 280
4X-RAY DIFFRACTION4( CHAIN B AND RESID 1:6 )B1 - 6
5X-RAY DIFFRACTION5( CHAIN B AND RESID 7:12 )B7 - 12
6X-RAY DIFFRACTION6( CHAIN B AND RESID 13:20 )B13 - 20
7X-RAY DIFFRACTION7( CHAIN B AND RESID 21:29 )B21 - 29
8X-RAY DIFFRACTION8( CHAIN B AND RESID 30:37 )B30 - 37
9X-RAY DIFFRACTION9( CHAIN B AND RESID 38:47 )B38 - 47
10X-RAY DIFFRACTION10( CHAIN B AND RESID 48:72 )B48 - 72
11X-RAY DIFFRACTION11( CHAIN B AND RESID 73:78 )B73 - 78
12X-RAY DIFFRACTION12( CHAIN B AND RESID 79:91 )B79 - 91
13X-RAY DIFFRACTION13( CHAIN B AND RESID 92:99 )B92 - 99
14X-RAY DIFFRACTION14( CHAIN C AND RESID 11:92 )C11 - 92
15X-RAY DIFFRACTION15( CHAIN C AND RESID 93:143 )C93 - 143
16X-RAY DIFFRACTION16( CHAIN C AND RESID 144:248 )C144 - 248
17X-RAY DIFFRACTION17( CHAIN D AND RESID 2:15 )D2 - 15
18X-RAY DIFFRACTION18( CHAIN D AND RESID 16:32 )D16 - 32
19X-RAY DIFFRACTION19( CHAIN D AND RESID 33:53 )D33 - 53
20X-RAY DIFFRACTION20( CHAIN D AND RESID 54:79 )D54 - 79
21X-RAY DIFFRACTION21( CHAIN D AND RESID 80:109 )D80 - 109
22X-RAY DIFFRACTION22( CHAIN D AND RESID 110:159 )D110 - 159
23X-RAY DIFFRACTION23( CHAIN D AND RESID 160:181 )D160 - 181

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