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- PDB-7r1h: Crystal structure of a flavodiiron protein D52K mutant in the red... -

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Basic information

Entry
Database: PDB / ID: 7r1h
TitleCrystal structure of a flavodiiron protein D52K mutant in the reduced state from Escherichia coli
ComponentsAnaerobic nitric oxide reductase flavorubredoxin
KeywordsOXIDOREDUCTASE / flavodiiron / nitrosative stress / ROS / FMN / iron
Function / homology
Function and homology information


nitric oxide reductase activity / oxidoreductase activity, acting on other nitrogenous compounds as donors / nitric oxide catabolic process / response to nitric oxide / FMN binding / electron transfer activity / oxidoreductase activity / iron ion binding / protein-containing complex / identical protein binding / cytoplasm
Similarity search - Function
Anaerobic nitric oxide reductase flavorubredoxin / Rubredoxin-oxygen oxidoreductase / ODP domain / ODP family beta lactamase / Rubredoxin domain / Rubredoxin / Rubredoxin-like domain / Rubredoxin-like domain profile. / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase ...Anaerobic nitric oxide reductase flavorubredoxin / Rubredoxin-oxygen oxidoreductase / ODP domain / ODP family beta lactamase / Rubredoxin domain / Rubredoxin / Rubredoxin-like domain / Rubredoxin-like domain profile. / Metallo-beta-lactamase superfamily / Metallo-beta-lactamase / Flavodoxin / Flavodoxin-like domain profile. / Flavodoxin/nitric oxide synthase / Ribonuclease Z/Hydroxyacylglutathione hydrolase-like / Flavoprotein-like superfamily
Similarity search - Domain/homology
: / FLAVIN MONONUCLEOTIDE / ISOPROPYL ALCOHOL / OXYGEN ATOM / OXYGEN MOLECULE / Anaerobic nitric oxide reductase flavorubredoxin
Similarity search - Component
Biological speciesEscherichia coli K-12 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.947 Å
AuthorsBorges, P.T. / Teixeira, M. / Romao, C.V. / Frazao, C.
Funding support Portugal, 1items
OrganizationGrant numberCountry
Foundation for Science and Technology (FCT) Portugal
CitationJournal: Acta Crystallogr D Struct Biol / Year: 2026
Title: Structures of variants of Escherichia coli flavodiiron-type nitric oxide reductase reveal changes in the di-iron site.
Authors: Borges, P.T. / Folgosa, F. / Martins, M.C. / Gotthard, G. / van der Linden, P. / Carpentier, P. / Teixeira, M. / Frazao, C. / Romao, C.V.
History
DepositionFeb 3, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 15, 2023Provider: repository / Type: Initial release
Revision 1.1May 1, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model / struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id ..._struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id
Revision 2.0Jun 17, 2026Group: Advisory / Atomic model ...Advisory / Atomic model / Author supporting evidence / Data collection / Database references / Derived calculations / Other / Refinement description / Source and taxonomy / Structure summary
Category: atom_site / atom_site_anisotrop ...atom_site / atom_site_anisotrop / cell / citation / citation_author / entity / entity_src_gen / pdbx_audit_support / pdbx_contact_author / pdbx_distant_solvent_atoms / pdbx_entity_instance_feature / pdbx_entry_details / pdbx_initial_refinement_model / pdbx_nonpoly_scheme / pdbx_refine_tls_group / pdbx_struct_assembly / pdbx_struct_special_symmetry / pdbx_validate_close_contact / refine / refine_hist / refine_ls_restr / refine_ls_restr_ncs / refine_ls_shell / reflns / struct_ncs_dom / struct_ncs_dom_lim
Item: _atom_site_anisotrop.U[1][2] / _atom_site_anisotrop.U[1][3] ..._atom_site_anisotrop.U[1][2] / _atom_site_anisotrop.U[1][3] / _atom_site_anisotrop.U[2][3] / _cell.angle_beta / _cell.length_a / _cell.length_b / _cell.length_c / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _entity.src_method / _entity_src_gen.gene_src_strain / _entity_src_gen.pdbx_gene_src_scientific_name / _pdbx_audit_support.country / _pdbx_distant_solvent_atoms.auth_seq_id / _pdbx_entry_details.has_protein_modification / _pdbx_initial_refinement_model.accession_code / _pdbx_initial_refinement_model.source_name / _pdbx_initial_refinement_model.type / _pdbx_nonpoly_scheme.asym_id / _pdbx_nonpoly_scheme.auth_seq_num / _pdbx_nonpoly_scheme.ndb_seq_num / _pdbx_nonpoly_scheme.pdb_seq_num / _pdbx_nonpoly_scheme.pdb_strand_id / _pdbx_refine_tls_group.beg_auth_asym_id / _pdbx_refine_tls_group.beg_auth_seq_id / _pdbx_refine_tls_group.end_auth_asym_id / _pdbx_refine_tls_group.end_auth_seq_id / _pdbx_struct_assembly.details / _pdbx_struct_special_symmetry.auth_seq_id / _refine.B_iso_max / _refine.B_iso_mean / _refine.B_iso_min / _refine.details / _refine.ls_R_factor_R_free / _refine.ls_R_factor_R_work / _refine.ls_R_factor_obs / _refine.ls_d_res_high / _refine_hist.cycle_id / _refine_hist.d_res_high / _refine_hist.number_atoms_total / _refine_hist.pdbx_B_iso_mean_ligand / _refine_hist.pdbx_B_iso_mean_solvent / _refine_hist.pdbx_number_atoms_ligand / _refine_hist.pdbx_number_atoms_protein / _refine_hist.pdbx_number_residues_total / _refine_ls_restr.dev_ideal / _refine_ls_restr.number / _refine_ls_restr.type / _refine_ls_shell.R_factor_R_free_error / _refine_ls_shell.d_res_high / _reflns.d_resolution_high
Provider: author / Type: Coordinate replacement

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Anaerobic nitric oxide reductase flavorubredoxin
B: Anaerobic nitric oxide reductase flavorubredoxin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)109,87412
Polymers108,6142
Non-polymers1,26010
Water13,007722
1
A: Anaerobic nitric oxide reductase flavorubredoxin
hetero molecules

A: Anaerobic nitric oxide reductase flavorubredoxin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)109,84612
Polymers108,6142
Non-polymers1,23210
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_555-x,y,-z1
Buried area6810 Å2
ΔGint-78 kcal/mol
Surface area28540 Å2
MethodPISA
2
B: Anaerobic nitric oxide reductase flavorubredoxin
hetero molecules

B: Anaerobic nitric oxide reductase flavorubredoxin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)109,90212
Polymers108,6142
Non-polymers1,28810
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
crystal symmetry operation2_555-x,y,-z1
Buried area6950 Å2
ΔGint-72 kcal/mol
Surface area28560 Å2
MethodPISA
Unit cell
Length a, b, c (Å)88.954, 64.861, 146.747
Angle α, β, γ (deg.)90.00, 91.53, 90.00
Int Tables number5
Space group name H-MI121
Components on special symmetry positions
IDModelComponents
11A-611-

HOH

21A-927-

HOH

31B-879-

HOH

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Components

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Protein , 1 types, 2 molecules AB

#1: Protein Anaerobic nitric oxide reductase flavorubredoxin / FlRd / FlavoRb


Mass: 54307.098 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli K-12 (bacteria) / Strain: K-12 / Gene: norV, flrD, ygaI, ygaJ, ygaK, b2710, JW2680 / Production host: Escherichia coli (E. coli) / References: UniProt: Q46877

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Non-polymers , 6 types, 732 molecules

#2: Chemical ChemComp-FMN / FLAVIN MONONUCLEOTIDE / RIBOFLAVIN MONOPHOSPHATE


Mass: 456.344 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C17H21N4O9P / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-FE / FE (III) ION


Mass: 55.845 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Fe / Feature type: SUBJECT OF INVESTIGATION
#4: Chemical ChemComp-O / OXYGEN ATOM


Mass: 15.999 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: O / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-OXY / OXYGEN MOLECULE


Mass: 31.999 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: O2 / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical ChemComp-IPA / ISOPROPYL ALCOHOL / 2-PROPANOL


Mass: 60.095 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C3H8O / Feature type: SUBJECT OF INVESTIGATION
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 722 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 1.94 Å3/Da / Density % sol: 36.64 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 5.6
Details: 0.1 M sodium citrate pH 5.6, 20-23% w/v PEG 4000 and 23-25% v/v 2-propanol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P14 (MX2) / Wavelength: 0.9763 Å
DetectorType: DECTRIS EIGER2 S 16M / Detector: PIXEL / Date: Nov 21, 2015
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 1.947→75.18 Å / Num. obs: 60345 / % possible obs: 98.2 % / Redundancy: 2.3 % / Biso Wilson estimate: 19.02 Å2 / CC1/2: 0.969 / Rpim(I) all: 0.083 / Net I/σ(I): 5.5
Reflection shellResolution: 1.96→2.05 Å / Num. unique obs: 8881 / CC1/2: 0.692 / Rpim(I) all: 0.357

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Processing

Software
NameVersionClassification
PHENIXdev_1436refinement
XSCALEdata scaling
PDB_EXTRACT3.27data extraction
XDSdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: FlRd

Resolution: 1.947→75.157 Å / SU ML: 0.18 / Cross valid method: FREE R-VALUE / σ(F): 32.04 / Phase error: 16.98 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.1869 60345 100 %
Rwork0.1869 60345 -
obs0.1869 60345 98.2 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Refinement stepCycle: LAST / Resolution: 1.947→75.157 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6496 0 8 722 7226
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0096691
X-RAY DIFFRACTIONf_angle_d0.659070
X-RAY DIFFRACTIONf_dihedral_angle_d11.3032425
X-RAY DIFFRACTIONf_chiral_restr0.026988
X-RAY DIFFRACTIONf_plane_restr0.0031172
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
1.947-1.96880.309515810.30951581X-RAY DIFFRACTION77
1.9688-1.99190.280118730.28011873X-RAY DIFFRACTION94
1.9919-2.01620.27820680.2782068X-RAY DIFFRACTION98
2.0162-2.04180.266219740.26621974X-RAY DIFFRACTION99
2.0418-2.06860.256920480.25692048X-RAY DIFFRACTION99
2.0686-2.0970.257319800.25731980X-RAY DIFFRACTION99
2.097-2.12690.248520280.24852028X-RAY DIFFRACTION99
2.1269-2.15870.239520340.23952034X-RAY DIFFRACTION99
2.1587-2.19240.223119720.22311972X-RAY DIFFRACTION99
2.1924-2.22840.213820520.21382052X-RAY DIFFRACTION99
2.2284-2.26680.201520050.20152005X-RAY DIFFRACTION99
2.2668-2.3080.204920020.20492002X-RAY DIFFRACTION99
2.308-2.35240.211520400.21152040X-RAY DIFFRACTION100
2.3524-2.40040.193120530.19312053X-RAY DIFFRACTION100
2.4004-2.45260.180820240.18082024X-RAY DIFFRACTION99
2.4526-2.50970.187320130.18732013X-RAY DIFFRACTION99
2.5097-2.57240.180120280.18012028X-RAY DIFFRACTION99
2.5724-2.6420.182220170.18222017X-RAY DIFFRACTION99
2.642-2.71970.184820430.18482043X-RAY DIFFRACTION99
2.7197-2.80750.184420290.18442029X-RAY DIFFRACTION99
2.8075-2.90790.176820110.17682011X-RAY DIFFRACTION99
2.9079-3.02430.172420280.17242028X-RAY DIFFRACTION99
3.0243-3.16190.169120140.16912014X-RAY DIFFRACTION99
3.1619-3.32870.167620490.16762049X-RAY DIFFRACTION99
3.3287-3.53720.15420320.1542032X-RAY DIFFRACTION99
3.5372-3.81030.151420640.15142064X-RAY DIFFRACTION99
3.8103-4.19370.144620410.14462041X-RAY DIFFRACTION99
4.1937-4.80050.144220470.14422047X-RAY DIFFRACTION99
4.8005-6.04770.168220670.16822067X-RAY DIFFRACTION99
6.0477-75.1570.195721280.19572128X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.0054-0.0046-0.0140.0341-0.02550.05240.0735-0.0963-0.0828-0.13050.016-0.02070.1085-0.08380.00090.1104-0.00710.00170.14550.0070.2083-28.5419-36.4746-10.0972
20.00610.0022-0.00490.00250.00170.00690.16520.0126-0.0147-0.04470.058-0.11060.0769-0.06300.1611-0.01010.01950.18780.05360.2168-11.0809-25.5157-4.8926
30.01220.0349-0.01590.102-0.01790.0554-0.0282-0.0273-0.12780.0548-0.00110.00060.0774-0.1278-0.00160.1259-0.0152-0.0110.12380.01950.1523-29.7392-30.8581-14.1935
40.10850.04980.03970.0753-0.02660.0830.01340.083-0.2025-0.1191-0.0123-0.08240.22140.0588-0.08360.23310.05610.00020.0558-0.03970.1725-25.0082-37.3287-22.1478
50.1771-0.0464-0.09130.1303-0.0270.0569-0.1182-0.1446-0.056-0.01480.0769-0.09470.2116-0.0193-0.00790.14740.03330.03620.11340.01080.152-26.6179-26.134-20.9577
60.03630.05510.00490.1022-0.05130.1220.00980.0540.0529-0.04280.0180.0388-0.01170.02490.02360.10210.0128-0.00710.09090.02360.0774-27.8859-18.8427-25.0325
70.1190.0453-0.08030.0663-0.05720.0791-0.0199-0.1708-0.1028-0.0269-0.0481-0.0199-0.0798-0.0787-0.07030.0399-0.02460.01380.12450.04890.1281-32.0469-25.1157-7.2651
80.01770.0075-0.01660.024-0.01240.02210.0728-0.1843-0.02940.08350.0954-0.0610.05760.03410.00480.1060.00150.02880.18610.03960.1656-21.6986-26.36194.4332
90.0323-0.00530.03580.0518-0.060.0630.0146-0.0769-0.0189-0.0362-0.022-0.0436-0.028-0.0873-0.00480.08650.01910.00780.1360.0290.0958-32.1299-19.5028-1.294
100.0163-0.03730.07230.1758-0.07950.4691-0.009-0.11940.0494-0.02570.03170.0669-0.0118-0.23540.05170.04450.0520.07510.20320.00560.1036-26.4876-15.97518.7476
110.15410.24670.03690.50460.0090.0145-0.0292-0.3545-0.23790.02050.1159-0.1750.1083-0.21150.09160.1235-0.0059-0.01610.21990.06180.0723-4.851-19.593619.3896
120.06410.03050.03860.0225-0.03920.0854-0.1006-0.0728-0.018-0.0250.0804-0.052-0.0150.00170.01160.08690.01560.01940.1019-0.01470.1205-4.2024-17.13088.3793
130.13960.08560.09420.05640.10250.3425-0.0389-0.02350.2037-0.16750.0028-0.02180.0068-0.1503-0.00820.16290.0478-0.00760.0920.02130.1672-12.7962-9.61626.1881
140.4191-0.1638-0.03030.3578-0.23850.329-0.2096-0.19350.3315-0.0227-0.0328-0.0669-0.2323-0.025-0.22740.16090.0337-0.01830.0671-0.08140.15841.5989-7.606913.6068
150.2690.2961-0.07490.3656-0.13610.1179-0.0726-0.28480.10190.1118-0.1648-0.1874-0.189-0.1205-0.46450.14660.12030.0450.2738-0.1768-0.0083-2.8975-12.720426.9424
160.0224-0.05780.00640.0888-0.02290.0045-0.0407-0.03950.02890.02140.0753-0.0558-0.18730.027900.21010.02090.01860.15420.03270.1482-5.4841-30.542429.8588
170.0091-0.0023-0.00180.0055-0.00280.0009-0.0267-0.0989-0.08710.04880.0858-0.011-0.124-0.0163-00.21690.05310.01320.1344-0.01580.184-1.306-41.480312.0971
180.0557-0.0692-0.00230.08820.02780.01940.03640.03580.0340.03780.02210.0841-0.1771-0.039700.24510.00270.01270.1585-0.00640.121-2.5024-36.15332.8979
190.0187-0.0014-0.0060.0378-0.02120.0133-0.12190.07610.1895-0.06620.1526-0.295-0.18470.17790.00010.2813-0.0129-0.01580.18490.0080.23326.7862-29.746132.8542
200.0966-0.0299-0.00140.0774-0.02120.13550.00620.05550.18130.0241-0.0602-0.0008-0.0528-0.2013-0.00870.13440.010.01570.12590.03330.14444.8877-40.899333.5416
210.0829-0.02870.03470.13020.10520.09360.0235-0.0253-0.096-0.00920.153-0.08530.07270.05670.08350.1708-0.0083-0.03010.14090.06340.12337.7943-48.196936.6574
220.18850.1292-0.05790.11640.02550.10910.0589-0.0846-0.0553-0.04560.06970.0873-0.0246-0.230.07460.15590.04370.01680.18840.04550.0886-9.7772-41.82331.3513
230.00860.003-0.0030.00810.00930.0092-0.01770.08640.0285-0.0449-0.05650.0395-0.1121-0.173800.1830.0136-0.02490.21770.04810.1809-14.7016-40.524316.6831
240.05970.0068-0.06360.11050.06710.13590.0859-0.10380.0396-0.13360.0626-0.0740.0132-0.25890.07030.127-0.0403-0.0190.21670.0850.1119-15.0014-47.39928.6023
250.1633-0.29320.12560.8452-0.1450.1256-0.1097-0.2286-0.02680.28180.0118-0.09390.0756-0.15430.01080.0626-0.03150.04710.37190.10760.2117-20.882-50.87318.6163
260.1007-0.08130.00160.14810.0530.07670.07910.07390.0802-0.15360.1404-0.0285-0.2073-0.48420.10830.1503-0.0028-0.04070.24870.06390.1451-19.2168-47.3406-5.4501
270.04650.00760.06320.03090.03750.10210.07430.02420.0022-0.00830.0802-0.07170.0972-0.11190.00420.1474-0.0222-0.01560.11980.00690.1435-9.3442-49.786-0.5222
280.15540.03110.01430.03420.03720.0869-0.042-0.0807-0.11510.0655-0.0135-0.14860.2295-0.0383-0.03120.2883-0.1004-0.09460.09570.12580.2065-11.7973-57.33098.0372
290.0312-0.04110.08490.0277-0.09520.25990.06220.003-0.2691-0.06610.09870.00470.3485-0.08050.18780.2306-0.1411-0.0301-0.1470.03790.1684-11.0027-59.3377-8.1403
300.0240.0680.0190.33620.08220.02510.1386-0.0973-0.2081-0.269-0.08180.13530.1993-0.32990.06670.1263-0.2693-0.190.32970.04210.1221-24.8283-54.1648-10.8713
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1(chain A and resid 2:20)
2X-RAY DIFFRACTION2(chain A and resid 21:28)
3X-RAY DIFFRACTION3(chain A and resid 29:52)
4X-RAY DIFFRACTION4(chain A and resid 53:69)
5X-RAY DIFFRACTION5(chain A and resid 70:91)
6X-RAY DIFFRACTION6(chain A and resid 92:131)
7X-RAY DIFFRACTION7(chain A and resid 132:186)
8X-RAY DIFFRACTION8(chain A and resid 187:194)
9X-RAY DIFFRACTION9(chain A and resid 195:239)
10X-RAY DIFFRACTION10(chain A and resid 240:251)
11X-RAY DIFFRACTION11(chain A and resid 252:291)
12X-RAY DIFFRACTION12(chain A and resid 292:327)
13X-RAY DIFFRACTION13(chain A and resid 328:340)
14X-RAY DIFFRACTION14(chain A and resid 341:375)
15X-RAY DIFFRACTION15(chain A and resid 376:400)
16X-RAY DIFFRACTION16(chain B and resid 2:20)
17X-RAY DIFFRACTION17(chain B and resid 21:28)
18X-RAY DIFFRACTION18(chain B and resid 29:52)
19X-RAY DIFFRACTION19(chain B and resid 53:69)
20X-RAY DIFFRACTION20(chain B and resid 70:91)
21X-RAY DIFFRACTION21(chain B and resid 92:131)
22X-RAY DIFFRACTION22(chain B and resid 132:186)
23X-RAY DIFFRACTION23(chain B and resid 187:194)
24X-RAY DIFFRACTION24(chain B and resid 195:239)
25X-RAY DIFFRACTION25(chain B and resid 240:251)
26X-RAY DIFFRACTION26(chain B and resid 252:291)
27X-RAY DIFFRACTION27(chain B and resid 292:327)
28X-RAY DIFFRACTION28(chain B and resid 328:340)
29X-RAY DIFFRACTION29(chain B and resid 341:375)
30X-RAY DIFFRACTION30(chain B and resid 376:400)

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