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- PDB-7qup: D. melanogaster 13-protofilament microtubule -

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Basic information

Entry
Database: PDB / ID: 7qup
TitleD. melanogaster 13-protofilament microtubule
Components
  • Tubulin alpha-1 chain
  • Tubulin beta-1 chain
KeywordsCELL CYCLE / Cytyoskeleton / microtubules / cytomotive filaments
Function / homology
Function and homology information


HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / astral microtubule / Neutrophil degranulation / lysosome localization / structural constituent of cytoskeleton / microtubule cytoskeleton organization ...HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand / COPI-mediated anterograde transport / COPI-independent Golgi-to-ER retrograde traffic / COPI-dependent Golgi-to-ER retrograde traffic / Kinesins / astral microtubule / Neutrophil degranulation / lysosome localization / structural constituent of cytoskeleton / microtubule cytoskeleton organization / spindle / mitotic cell cycle / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / microtubule / hydrolase activity / GTPase activity / centrosome / GTP binding / perinuclear region of cytoplasm / nucleus / metal ion binding / cytoplasm
Similarity search - Function
Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain ...Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / Tubulin alpha-1 chain / Tubulin beta-1 chain
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsWagstaff, J. / Planelles-Herrero, V.J. / Derivery, E. / Lowe, J.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
Wellcome Trust202754/Z/16/Z United Kingdom
Medical Research Council (MRC, United Kingdom)U105184326 United Kingdom
CitationJournal: Sci Adv / Year: 2023
Title: Diverse cytomotive actins and tubulins share a polymerization switch mechanism conferring robust dynamics.
Authors: James Mark Wagstaff / Vicente José Planelles-Herrero / Grigory Sharov / Aisha Alnami / Frank Kozielski / Emmanuel Derivery / Jan Löwe /
Abstract: Protein filaments are used in myriads of ways to organize other molecules within cells. Some filament-forming proteins couple the hydrolysis of nucleotides to their polymerization cycle, thus ...Protein filaments are used in myriads of ways to organize other molecules within cells. Some filament-forming proteins couple the hydrolysis of nucleotides to their polymerization cycle, thus powering the movement of other molecules. These filaments are termed cytomotive. Only members of the actin and tubulin protein superfamilies are known to form cytomotive filaments. We examined the basis of cytomotivity via structural studies of the polymerization cycles of actin and tubulin homologs from across the tree of life. We analyzed published data and performed structural experiments designed to disentangle functional components of these complex filament systems. Our analysis demonstrates the existence of shared subunit polymerization switches among both cytomotive actins and tubulins, i.e., the conformation of subunits switches upon assembly into filaments. These cytomotive switches can explain filament robustness, by enabling the coupling of kinetic and structural polarities required for cytomotive behaviors and by ensuring that single cytomotive filaments do not fall apart.
History
DepositionJan 18, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 2, 2022Provider: repository / Type: Initial release
Revision 1.1Apr 19, 2023Group: Database references / Refinement description
Category: citation / citation_author / pdbx_initial_refinement_model
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Oct 4, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / em_3d_fitting_list
Item: _em_3d_fitting_list.accession_code / _em_3d_fitting_list.initial_refinement_model_id ..._em_3d_fitting_list.accession_code / _em_3d_fitting_list.initial_refinement_model_id / _em_3d_fitting_list.source_name / _em_3d_fitting_list.type

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
10A: Tubulin alpha-1 chain
10B: Tubulin beta-1 chain
10C: Tubulin alpha-1 chain
10D: Tubulin beta-1 chain
10E: Tubulin alpha-1 chain
11A: Tubulin alpha-1 chain
11B: Tubulin beta-1 chain
11C: Tubulin alpha-1 chain
11D: Tubulin beta-1 chain
11E: Tubulin alpha-1 chain
12A: Tubulin alpha-1 chain
12B: Tubulin beta-1 chain
12C: Tubulin alpha-1 chain
12D: Tubulin beta-1 chain
12E: Tubulin alpha-1 chain
13A: Tubulin alpha-1 chain
13B: Tubulin beta-1 chain
13C: Tubulin alpha-1 chain
13D: Tubulin beta-1 chain
13E: Tubulin alpha-1 chain
1A: Tubulin alpha-1 chain
1B: Tubulin beta-1 chain
1C: Tubulin alpha-1 chain
1D: Tubulin beta-1 chain
1E: Tubulin alpha-1 chain
2A: Tubulin alpha-1 chain
2B: Tubulin beta-1 chain
2C: Tubulin alpha-1 chain
2D: Tubulin beta-1 chain
2E: Tubulin alpha-1 chain
3A: Tubulin alpha-1 chain
3B: Tubulin beta-1 chain
3C: Tubulin alpha-1 chain
3D: Tubulin beta-1 chain
3E: Tubulin alpha-1 chain
4A: Tubulin alpha-1 chain
4B: Tubulin beta-1 chain
4C: Tubulin alpha-1 chain
4D: Tubulin beta-1 chain
4E: Tubulin alpha-1 chain
5A: Tubulin alpha-1 chain
5B: Tubulin beta-1 chain
5C: Tubulin alpha-1 chain
5D: Tubulin beta-1 chain
5E: Tubulin alpha-1 chain
6A: Tubulin alpha-1 chain
6B: Tubulin beta-1 chain
6C: Tubulin alpha-1 chain
6D: Tubulin beta-1 chain
6E: Tubulin alpha-1 chain
7A: Tubulin alpha-1 chain
7B: Tubulin beta-1 chain
7C: Tubulin alpha-1 chain
7D: Tubulin beta-1 chain
7E: Tubulin alpha-1 chain
8A: Tubulin alpha-1 chain
8B: Tubulin beta-1 chain
8C: Tubulin alpha-1 chain
8D: Tubulin beta-1 chain
8E: Tubulin alpha-1 chain
9A: Tubulin alpha-1 chain
9B: Tubulin beta-1 chain
9C: Tubulin alpha-1 chain
9D: Tubulin beta-1 chain
9E: Tubulin alpha-1 chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)3,330,720169
Polymers3,297,84565
Non-polymers32,875104
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

#1: Protein ...
Tubulin alpha-1 chain


Mass: 52772.324 Da / Num. of mol.: 39
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: alphaTub84B, tubA84B, CG1913 / Production host: Drosophila melanogaster (fruit fly) / References: UniProt: P06603
#2: Protein ...
Tubulin beta-1 chain / Beta-1-tubulin


Mass: 47681.707 Da / Num. of mol.: 26 / Source method: isolated from a natural source / Source: (natural) Drosophila melanogaster (fruit fly) / References: UniProt: Q24560
#3: Chemical...
ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 39 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#4: Chemical...
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 39 / Source method: obtained synthetically / Formula: Mg
#5: Chemical...
ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 26 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: FILAMENT / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: Microtubule / Type: COMPLEX / Entity ID: #1-#2 / Source: RECOMBINANT
Molecular weightValue: 1.170 MDa / Experimental value: NO
Source (natural)Organism: Drosophila melanogaster (fruit fly)
Source (recombinant)Organism: Drosophila melanogaster (fruit fly)
Buffer solutionpH: 6.9
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: GOLD / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 1000 nm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 32 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k)

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Processing

SoftwareName: UCSF ChimeraX / Version: 1.4/v9 / Classification: model building / URL: https://www.rbvi.ucsf.edu/chimerax/ / Os: macOS / Type: package
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
3D reconstructionResolution: 3.8 Å / Resolution method: FSC 0.5 CUT-OFF / Num. of particles: 39594 / Algorithm: BACK PROJECTION
Details: Resolution was determined against the atomic model, suing FSC 0.5
Symmetry type: POINT
Atomic model buildingProtocol: OTHER / Space: REAL
Atomic model buildingPDB-ID: 3J1T

3j1t


Accession code: 3J1T / Source name: PDB / Type: experimental model

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