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Yorodumi- PDB-7qrs: Structural insight into the Scribble PDZ domains interaction with... -
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-Basic information
Entry | Database: PDB / ID: 7qrs | ||||||
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Title | Structural insight into the Scribble PDZ domains interaction with the oncogenic Human T-cell lymphotrophic virus-1 (HTLV-1) Tax1 | ||||||
Components |
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Keywords | VIRAL PROTEIN / Human T lymphotrophic virus-1 / HTLV-1 / cell polarity / isothermal titration calorimetry / Tax1 / PDZ / scribble | ||||||
Function / homology | Function and homology information symbiont-mediated perturbation of host exit from mitosis / : / neurotransmitter receptor transport postsynaptic membrane to endosome / symbiont-mediated perturbation of host cell cycle G0/G1 transition checkpoint / extrinsic component of postsynaptic density membrane / establishment of T cell polarity / apoptotic process involved in morphogenesis / establishment of apical/basal cell polarity / cochlear nucleus development / synaptic vesicle targeting ...symbiont-mediated perturbation of host exit from mitosis / : / neurotransmitter receptor transport postsynaptic membrane to endosome / symbiont-mediated perturbation of host cell cycle G0/G1 transition checkpoint / extrinsic component of postsynaptic density membrane / establishment of T cell polarity / apoptotic process involved in morphogenesis / establishment of apical/basal cell polarity / cochlear nucleus development / synaptic vesicle targeting / Scrib-APC-beta-catenin complex / astrocyte cell migration / polarized epithelial cell differentiation / myelin sheath abaxonal region / neurotransmitter receptor transport, endosome to postsynaptic membrane / protein localization to adherens junction / cell-cell contact zone / mammary gland duct morphogenesis / post-anal tail morphogenesis / establishment or maintenance of epithelial cell apical/basal polarity / activation of GTPase activity / regulation of postsynaptic neurotransmitter receptor internalization / auditory receptor cell stereocilium organization / RND2 GTPase cycle / RND3 GTPase cycle / positive regulation of receptor recycling / positive chemotaxis / receptor clustering / RHOJ GTPase cycle / RHOQ GTPase cycle / negative regulation of activated T cell proliferation / symbiont-mediated perturbation of host cell cycle G1/S transition checkpoint / CDC42 GTPase cycle / synaptic vesicle endocytosis / negative regulation of mitotic cell cycle / immunological synapse / signaling adaptor activity / regulation of mRNA stability / Asymmetric localization of PCP proteins / neural tube closure / adherens junction / wound healing / cell-cell adhesion / SH3 domain binding / cell migration / cell-cell junction / positive regulation of type II interferon production / presynapse / cell junction / lamellipodium / basolateral plasma membrane / cellular response to lipopolysaccharide / cell population proliferation / host cell cytoplasm / postsynaptic density / cadherin binding / positive regulation of apoptotic process / negative regulation of gene expression / glutamatergic synapse / host cell nucleus / positive regulation of DNA-templated transcription / DNA binding / extracellular exosome / nucleoplasm / metal ion binding / plasma membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Human T-cell leukemia virus type I | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.77 Å | ||||||
Authors | Javorsky, A. / Soares da Costa, T.P. / Mackie, E.R. / Humbert, P.O. / Kvansakul, M. | ||||||
Funding support | 1items
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Citation | Journal: Febs J. / Year: 2023 Title: Structural insight into the Scribble PDZ domains interaction with the oncogenic Human T-cell lymphotrophic virus-1 (HTLV-1) Tax1 PBM. Authors: Javorsky, A. / Maddumage, J.C. / Mackie, E.R.R. / Soares da Costa, T.P. / Humbert, P.O. / Kvansakul, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7qrs.cif.gz | 114.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7qrs.ent.gz | 72 KB | Display | PDB format |
PDBx/mmJSON format | 7qrs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/qr/7qrs ftp://data.pdbj.org/pub/pdb/validation_reports/qr/7qrs | HTTPS FTP |
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-Related structure data
Related structure data | 7qrtC 7qs8C 5vwcS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 12380.901 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: SCRIB, CRIB1, KIAA0147, LAP4, SCRB1, VARTUL / Production host: Escherichia coli (E. coli) / References: UniProt: Q14160 #2: Protein/peptide | Mass: 1048.172 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Human T-cell leukemia virus type I / Strain: isolate Caribbea HS-35 subtype A / Gene: tax / Production host: synthetic construct (others) / References: UniProt: P14079 #3: Chemical | ChemComp-ACT / #4: Chemical | ChemComp-GOL / | #5: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.86 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop Details: 0.1M Zinc chloride, 0.1M Sodium acetate ph5, 20% w/v PEG 1500 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 1, 2020 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 1.77→46.1 Å / Num. obs: 25599 / % possible obs: 99.3 % / Redundancy: 2.7 % / Biso Wilson estimate: 31.33 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.056 / Net I/σ(I): 16.48 |
Reflection shell | Resolution: 1.77→1.8 Å / Rmerge(I) obs: 0.76 / Num. unique obs: 2557 / CC1/2: 0.43 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 5VWC Resolution: 1.77→28.14 Å / SU ML: 0.2592 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 33.7626 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 42.51 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.77→28.14 Å
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Refine LS restraints |
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LS refinement shell |
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