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- PDB-7qng: Structure of a MHC I-Tapasin-ERp57 complex -

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Basic information

Entry
Database: PDB / ID: 7qng
TitleStructure of a MHC I-Tapasin-ERp57 complex
Components
  • Beta-2-microglobulin
  • H-2 class I histocompatibility antigen, D-B alpha chain
  • Protein disulfide-isomerase A3
  • Tapasin
KeywordsIMMUNE SYSTEM / MHC I / chaperone complex / endoplasmic reticulum / peptide editing
Function / homology
Function and homology information


MHC class Ib protein complex assembly / peptide antigen stabilization / Tapasin-ERp57 complex / Calnexin/calreticulin cycle / MHC class I protein complex binding / TAP1 binding / TAP2 binding / protein disulfide-isomerase / disulfide oxidoreductase activity / protein folding in endoplasmic reticulum ...MHC class Ib protein complex assembly / peptide antigen stabilization / Tapasin-ERp57 complex / Calnexin/calreticulin cycle / MHC class I protein complex binding / TAP1 binding / TAP2 binding / protein disulfide-isomerase / disulfide oxidoreductase activity / protein folding in endoplasmic reticulum / regulation of protein complex stability / phospholipase C activity / retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum / positive regulation of extrinsic apoptotic signaling pathway / cellular response to interleukin-7 / TAP complex binding / protein disulfide isomerase activity / MHC class I protein binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent / protein-disulfide reductase activity / beta-2-microglobulin binding / extrinsic apoptotic signaling pathway / protein folding chaperone / phagocytic vesicle / endoplasmic reticulum-Golgi intermediate compartment membrane / response to endoplasmic reticulum stress / positive regulation of ferrous iron binding / positive regulation of transferrin receptor binding / positive regulation of receptor binding / early endosome lumen / Nef mediated downregulation of MHC class I complex cell surface expression / DAP12 interactions / negative regulation of receptor binding / antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent / antigen processing and presentation of endogenous peptide antigen via MHC class Ib / lumenal side of endoplasmic reticulum membrane / cellular response to iron ion / Endosomal/Vacuolar pathway / Antigen Presentation: Folding, assembly and peptide loading of class I MHC / peptide binding / cellular response to iron(III) ion / antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent / negative regulation of forebrain neuron differentiation / regulation of erythrocyte differentiation / ER to Golgi transport vesicle membrane / peptide antigen assembly with MHC class I protein complex / regulation of iron ion transport / response to molecule of bacterial origin / MHC class I peptide loading complex / HFE-transferrin receptor complex / T cell mediated cytotoxicity / antigen processing and presentation of endogenous peptide antigen via MHC class I / positive regulation of T cell cytokine production / MHC class I protein complex / multicellular organismal-level iron ion homeostasis / platelet aggregation / negative regulation of neurogenesis / peptide antigen assembly with MHC class II protein complex / positive regulation of receptor-mediated endocytosis / MHC class II protein complex / cellular response to nicotine / positive regulation of T cell mediated cytotoxicity / specific granule lumen / recycling endosome membrane / phagocytic vesicle membrane / positive regulation of cellular senescence / peptide antigen binding / negative regulation of epithelial cell proliferation / antigen processing and presentation of exogenous peptide antigen via MHC class II / Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell / Interferon gamma signaling / positive regulation of immune response / Modulation by Mtb of host immune system / sensory perception of smell / unfolded protein binding / melanosome / positive regulation of T cell activation / protein folding / positive regulation of protein binding / tertiary granule lumen / DAP12 signaling / negative regulation of neuron projection development / MHC class II protein complex binding / late endosome membrane / iron ion transport / ER-Phagosome pathway / early endosome membrane / T cell differentiation in thymus / protein refolding / regulation of gene expression / protein-containing complex assembly / protein homotetramerization / intracellular iron ion homeostasis / adaptive immune response / amyloid fibril formation / molecular adaptor activity / learning or memory / immune response / Amyloid fiber formation / endoplasmic reticulum lumen
Similarity search - Function
Protein disulfide-isomerase A3, first redox inactive TRX-like domain b / Tapasin / Protein disulphide isomerase / Thioredoxin-like domain / Disulphide isomerase / : / Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. ...Protein disulfide-isomerase A3, first redox inactive TRX-like domain b / Tapasin / Protein disulphide isomerase / Thioredoxin-like domain / Disulphide isomerase / : / Thioredoxin / Thioredoxin, conserved site / Thioredoxin family active site. / Thioredoxin domain profile. / Thioredoxin domain / MHC class I, alpha chain, C-terminal / MHC_I C-terminus / MHC class I alpha chain, alpha1 alpha2 domains / Class I Histocompatibility antigen, domains alpha 1 and 2 / Beta-2-Microglobulin / MHC class I-like antigen recognition-like / MHC class I-like antigen recognition-like superfamily / MHC classes I/II-like antigen recognition protein / Immunoglobulin/major histocompatibility complex, conserved site / Immunoglobulins and major histocompatibility complex proteins signature. / Immunoglobulin C-Type / Immunoglobulin C1-set / Immunoglobulin C1-set domain / Thioredoxin-like superfamily / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Tapasin / H-2 class I histocompatibility antigen, D-B alpha chain / Protein disulfide-isomerase A3 / Beta-2-microglobulin
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å
AuthorsMueller, I.K. / Thomas, C. / Trowitzsch, S. / Tampe, R.
Funding support Germany, European Union, 2items
OrganizationGrant numberCountry
German Research Foundation (DFG)TA157/12-1 Germany
European Research Council (ERC)789121European Union
CitationJournal: Nat Commun / Year: 2022
Title: Structure of an MHC I-tapasin-ERp57 editing complex defines chaperone promiscuity.
Authors: Muller, I.K. / Winter, C. / Thomas, C. / Spaapen, R.M. / Trowitzsch, S. / Tampe, R.
History
DepositionDec 20, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 28, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tapasin
B: Protein disulfide-isomerase A3
C: H-2 class I histocompatibility antigen, D-B alpha chain
D: Beta-2-microglobulin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)146,3975
Polymers145,8104
Non-polymers5871
Water93752
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10410 Å2
ΔGint-20 kcal/mol
Surface area58080 Å2
Unit cell
Length a, b, c (Å)74.760, 168.530, 187.050
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number18
Space group name H-MP22121
Space group name HallP22ab(z,x,y)
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y+1/2,-z+1/2
#4: -x,-y+1/2,z+1/2

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Components

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Protein , 4 types, 4 molecules ABCD

#1: Protein Tapasin / TPN / TPSN / NGS-17 / TAP-associated protein / TAP-binding protein


Mass: 44881.969 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: TAPBP, NGS17, TAPA / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: O15533
#2: Protein Protein disulfide-isomerase A3 / 58 kDa glucose-regulated protein / 58 kDa microsomal protein / p58 / Disulfide isomerase ER-60 / ...58 kDa glucose-regulated protein / 58 kDa microsomal protein / p58 / Disulfide isomerase ER-60 / Endoplasmic reticulum resident protein 57 / ER protein 57 / ERp57 / Endoplasmic reticulum resident protein 60 / ER protein 60 / ERp60


Mass: 56830.199 Da / Num. of mol.: 1 / Mutation: C60A
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PDIA3, ERP57, ERP60, GRP58 / Production host: Spodoptera frugiperda (fall armyworm) / References: UniProt: P30101, protein disulfide-isomerase
#3: Protein H-2 class I histocompatibility antigen, D-B alpha chain / H-2D(B)


Mass: 32218.895 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: H2-D1 / Production host: Escherichia coli (E. coli) / References: UniProt: P01899
#4: Protein Beta-2-microglobulin


Mass: 11879.356 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: B2M, CDABP0092, HDCMA22P / Production host: Escherichia coli (E. coli) / References: UniProt: P61769

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Sugars / Non-polymers , 2 types, 53 molecules

#5: Polysaccharide beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta- ...beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 586.542 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
DManpb1-4DGlcpNAcb1-4DGlcpNAcb1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/2,3,2/[a2122h-1b_1-5_2*NCC/3=O][a1122h-1b_1-5]/1-1-2/a4-b1_b4-c1WURCSPDB2Glycan 1.1.0
[][D-1-deoxy-GlcpNAc]{[(4+1)][b-D-GlcpNAc]{[(4+1)][b-D-Manp]{}}}LINUCSPDB-CARE
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 52 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.04 Å3/Da / Density % sol: 69.59 %
Crystal growTemperature: 291.15 K / Method: vapor diffusion, sitting drop
Details: 100 mM Gly-Gly, AMPD, pH 8.5, 300 mM lithium sulfate, 300 mM sodium sulfate, 300 mM potassium sulfate, 20% (v/v) PEG8000, 40% (v/v) 1,5-pentanediol

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 1.033 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 1, 2020
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.033 Å / Relative weight: 1
ReflectionResolution: 2.7→48 Å / Num. obs: 65719 / % possible obs: 99.76 % / Redundancy: 5.9 % / Biso Wilson estimate: 87.61 Å2 / CC1/2: 0.998 / Net I/σ(I): 14.9
Reflection shellResolution: 2.7→2.797 Å / Num. unique obs: 6491 / CC1/2: 0.443

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Processing

Software
NameVersionClassification
PDB-REDOrefinement
PHENIX1.20.1_4487refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6ENY

6eny


Resolution: 2.7→48 Å / SU ML: 0.4301 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 25.0761
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2289 3284 5 %
Rwork0.1983 62368 -
obs0.1999 65719 99.76 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 106.17 Å2
Refinement stepCycle: LAST / Resolution: 2.7→48 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9621 0 48 52 9721
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00949942
X-RAY DIFFRACTIONf_angle_d1.356513507
X-RAY DIFFRACTIONf_chiral_restr0.08151425
X-RAY DIFFRACTIONf_plane_restr0.00491774
X-RAY DIFFRACTIONf_dihedral_angle_d12.31713698
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.7-2.740.36851400.3332673X-RAY DIFFRACTION99.86
2.74-2.780.32891420.31952696X-RAY DIFFRACTION99.79
2.78-2.830.32891410.31242662X-RAY DIFFRACTION100
2.83-2.880.48541420.42062709X-RAY DIFFRACTION99.96
2.88-2.930.43421400.40932655X-RAY DIFFRACTION99.96
2.93-2.990.38061420.34732699X-RAY DIFFRACTION99.93
2.99-3.050.3241390.29662646X-RAY DIFFRACTION99.89
3.05-3.110.29751440.26522721X-RAY DIFFRACTION99.79
3.11-3.190.30621400.25062670X-RAY DIFFRACTION100
3.19-3.270.28091420.23512697X-RAY DIFFRACTION99.82
3.27-3.350.29531420.23692681X-RAY DIFFRACTION99.89
3.35-3.450.29121400.26382678X-RAY DIFFRACTION99.65
3.45-3.560.27311430.25112716X-RAY DIFFRACTION99.9
3.56-3.690.28191420.20882693X-RAY DIFFRACTION99.93
3.69-3.840.23341410.19032695X-RAY DIFFRACTION99.75
3.84-4.010.20261430.18932716X-RAY DIFFRACTION99.79
4.01-4.220.20761430.18112704X-RAY DIFFRACTION99.93
4.23-4.490.18691430.15892725X-RAY DIFFRACTION99.83
4.49-4.840.17381450.14342734X-RAY DIFFRACTION99.9
4.84-5.320.17871450.15362760X-RAY DIFFRACTION99.83
5.32-6.090.20031450.17652751X-RAY DIFFRACTION99.76
6.09-7.670.20891470.19082797X-RAY DIFFRACTION99.56
7.67-480.20231530.16742890X-RAY DIFFRACTION98.42
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.09891198150.6139797495460.3657597657547.33771276860.4972921724412.92376667952-0.0405762598555-0.1538401292690.08608880077970.348167541171-0.154469485806-0.505790321279-0.2959531832350.2706213693830.2237680246580.457320040189-0.0230114989151-0.07348625316620.6076255595550.07481744926510.5846380049641.23443008571.547090390732.8117343186
25.44674111821-0.6465718507953.163342975143.3701987374-0.5481408165645.701063811470.110143605938-0.0467723093304-0.1640969926650.140343427021-0.0827971288741-0.2449088705250.2233866071170.235103080211-0.02718947262390.400132573267-0.001738388149690.07329669628680.3931084474370.03820498947950.45884148011534.397924122854.085386262223.4211643095
37.53456325566-2.06034147781.136869629257.09618753112-3.182805799427.24347370265-0.1085062807920.7851911493410.434225959908-0.7726552269580.2096397624380.586835288493-0.295701556293-0.555584523277-0.107158014240.606479757996-0.0485144855341-0.08197367454340.6475186142340.01212694087030.5506759637265.5979509998670.4681383787-6.25705231943
44.72327063975.98148198517-4.520326599261.99995415368-3.475765986485.621636046020.347914966331-0.257482238279-0.159537184514-1.50173432874-1.21325944486-5.734042785521.35403517761-0.1103538452320.8377593969252.707869747650.5805213726230.07082804812371.696159079980.03167314322322.334313875153.5265655309861.4599316799-21.5864070561
54.481069418760.65450752668-1.031543826017.36893130782-3.242095017733.39889671486-0.137227065748-0.7248003678830.1170599082751.99201610852-0.0133123487917-0.218717448125-0.8155806935090.1709959261420.1409569092281.514921921350.176611044327-0.1505614488080.9335119409970.1004174458640.66741345224941.877194935654.463370762263.2779037225
60.8778459322040.7788008932421.44249560412.597789441692.231369594456.0791190639-0.09097496227010.1205214329250.105380093114-0.723717314027-0.1191078657470.481067713254-0.735319913510.02180986787410.2359903738540.9892630470090.143715885996-0.1951200764110.5977261091120.04080817344150.70711397971541.282401812721.411244675162.3354016758
72.592877134170.4709774130750.8960476881563.78018725434-1.401333616781.748157303440.7604372971730.673734866615-0.89934696869-0.728879824518-0.353947938911-1.238085350742.109327569351.2695516373-0.3123684297451.63254981610.588507369578-0.1529390896991.11617825836-0.1277170829341.1453733597443.808608411129.123217754916.5005260206
87.560603903711.951723666510.58513861155.87957105682-0.7269265827483.141279641230.188610961441-0.8687350224480.2277830700920.784503600878-0.09240254594090.914805589897-0.325874187376-0.703361651282-0.1155155782950.6297644159030.1363553086190.1390361440540.881656318887-0.0460848627570.6841268767812.3187371514366.945289383236.9536203299
96.84650425319-0.1823907112850.06422293961728.40215645785-1.994308260754.88558147362-0.027413840229-0.347219086985-0.6925155814490.2053345065070.02624215107070.1058767318320.13015582952-0.06864013407750.02739668402960.4690357005440.02914816235610.05560560729790.6416698366410.02362856845320.576375155918.329388933958.596385856134.6925568971
105.042487926641.12710904933-0.04015138227991.13712283835-1.04590623434.837013840470.495822877765-1.1483167091-1.340243883370.1864175340340.009860003395160.3404687542410.637857268276-1.17722352735-0.4362491907920.8345565088690.0372619131396-0.03894479525990.7237594904620.1682720511261.33326252646-1.3731697309149.832069025229.2334814876
117.33992458619-0.6178203908420.7726260135153.56326298214-0.2712158536487.492612496240.3866836647940.824256740212-1.17519850964-0.544527260611-0.07474252997240.5653646757391.25909428905-0.417432416985-0.2938715167070.824109981815-0.219531087478-0.1756906376420.828367735849-0.1082443868820.969299306712-3.3126424878946.92154792494.62243383732
125.26264892882-0.1738889236755.204155662723.77898417824-0.8853711163365.28403719631-1.053535360270.876982065044-0.427126252623-0.7806453466870.529951958927-0.0533563572566-0.5469033337230.09087158518050.5185518486880.519151665663-0.01187433823020.01207364667060.7700253103640.209498608970.79508804222511.208345671570.300082551217.922813288
136.96296938192-4.073441428565.768988346755.62903048626-0.9942068614119.38232136206-0.000452856647550.0814290517838-1.63692947356-0.088521777380.3880128192350.8325326171780.353217520707-0.965712985917-0.3191246026080.634645879502-0.06769879864780.06010577253680.7937943333750.07294262879560.781779766964-4.7171947429863.569542007110.7895801591
144.7817933649-3.438070172021.654385461953.827703583710.6335585853483.02159559581.1213216429-0.000905801040848-0.90144247736-0.633771272930.7342273999082.437418588650.689002923901-1.60966240217-1.834554327110.823019159186-0.226380854515-0.1421164583541.265647509760.1123200815091.55947032275-19.912943078358.99766878697.85169831987
150.0936584370245-0.4788895683330.2140421186823.301011854660.4719960776942.931487031290.515646295795-0.46363462880.325460569915-0.261926869535-0.2571435931880.9227330371850.320980211434-1.30026713302-0.2032145875260.45974556983-0.131034078652-0.04474473434331.135995484580.1609133713110.927106262046-8.0539142374164.973628495714.8220917214
162.00932068728-1.97506757991.985290657574.99943605348-1.678710929882.03410186195-0.432137379423-0.1964867953131.13361505864-0.2220337076010.116744161105-0.0022118201191-0.932732056017-0.4933679276810.2967408722170.6856835195460.1188719593150.03522813921110.8728003099740.1326740967060.851426524667-2.8893959311274.58294901516.4451562008
179.23388352735-2.450701531854.621588829884.82401264616-3.006646183697.80268436421-0.4008981903170.48266166594-0.03057477507890.7177328780250.3275129185271.18279133473-1.25778155259-2.039368802230.03609185548510.5957714886840.1990580201280.1016373507691.048901557990.1407188070091.24275137587-14.250435551276.007746993114.2253830292
183.24241391109-2.581987740293.398814659672.83653123248-0.5606524130559.42947044587-1.00826370143-1.112726908211.264274680241.400198161731.1604024850.404346014244-0.119291221193-0.881275364491-0.1143358793380.7951740908610.04120491243380.07875807684270.6721766656080.001936488046961.09937832073-2.6941612847866.948794892926.6438195984
199.32892438877-2.60826513445.358171536282.27764375939-0.9948756256993.3382796114-0.5258858052010.1370367183990.400062837539-0.01158941638750.2368366174350.743207861212-0.128474134108-0.9645011091720.03220143046080.558210491993-0.03559548835680.03198609898711.08662756430.1311150340441.00829322474-8.2530040338766.350487698414.6467818367
206.734659554067.423444054475.596882899338.139837980696.142480102374.65379667293-0.0941115243685-0.1341553278081.00940416451-0.150647468846-0.17588453650.567952197435-0.411535539127-0.6646735906980.405138850850.5213374342610.115441327295-0.03805944916071.15069455490.1604277895690.906813115317-6.0565383366273.81538839689.7475282462
212.998154552570.6328863097173.520117178374.723001272011.445924138017.52357208294-0.2728654818710.586159309066-0.0219232161656-0.2459887295740.1874015896610.118555945272-0.126011861296-0.3358022401160.1759477192950.453153701740.00529604263633-0.01438819560950.9151852918060.1032605248210.80543620184-2.4565129861470.0211719046.69867966187
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 1 through 143 )AA1 - 1431 - 143
22chain 'A' and (resid 144 through 269 )AA144 - 269144 - 269
33chain 'A' and (resid 270 through 380 )AA270 - 380270 - 375
44chain 'A' and (resid 381 through 381 )AB3811
55chain 'B' and (resid 1 through 108 )BF1 - 1081 - 105
66chain 'B' and (resid 109 through 334 )BF109 - 334106 - 331
77chain 'B' and (resid 335 through 467 )BF335 - 467332 - 464
88chain 'C' and (resid 1 through 103 )CG1 - 1031 - 101
99chain 'C' and (resid 104 through 151 )CG104 - 151102 - 149
1010chain 'C' and (resid 152 through 194 )CG152 - 194150 - 192
1111chain 'C' and (resid 195 through 276 )CG195 - 276193 - 274
1212chain 'D' and (resid 0 through 5 )DH0 - 51 - 6
1313chain 'D' and (resid 6 through 11 )DH6 - 117 - 12
1414chain 'D' and (resid 12 through 19 )DH12 - 1913 - 20
1515chain 'D' and (resid 20 through 30 )DH20 - 3021 - 31
1616chain 'D' and (resid 31 through 41 )DH31 - 4132 - 42
1717chain 'D' and (resid 42 through 51 )DH42 - 5143 - 52
1818chain 'D' and (resid 52 through 56 )DH52 - 5653 - 57
1919chain 'D' and (resid 57 through 77 )DH57 - 7758 - 78
2020chain 'D' and (resid 78 through 83 )DH78 - 8379 - 84
2121chain 'D' and (resid 84 through 99 )DH84 - 9985 - 100

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