Component-ID: _ / Ens-ID: 1 / Beg auth comp-ID: PRO / Beg label comp-ID: PRO / End auth comp-ID: THR / End label comp-ID: THR / Refine code: _ / Auth seq-ID: 11 - 123 / Label seq-ID: 3 - 115
Dom-ID
Auth asym-ID
Label asym-ID
1
A
A
2
B
B
-
Components
#1: Protein
Transthyretin / ATTR / Prealbumin / TBPA
Mass: 13090.689 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Evidence for alternative conformation(s) for loop A:100-103 at crystal packing interface. Density not convincing enough to model; set occupancy to 50%. Source: (gene. exp.) Homo sapiens (human) / Gene: TTR, PALB / Production host: Escherichia coli (E. coli) / References: UniProt: P02766
Mass: 18.015 Da / Num. of mol.: 158 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 2.26 Å3/Da / Density % sol: 45.66 %
Crystal grow
Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.5 Details: TTR:SB11 complex at 5.2mg/ml in 10mM sodium phosphate pH7.6 and 100mM potassium chloride was mixed 1:1 with 1.3-1.6M sodium citrate and 3.5% glycerol.
-
Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source
Source: SYNCHROTRON / Site: MAX IV / Beamline: BioMAX / Wavelength: 0.97993 Å
Detector
Type: DECTRIS EIGER2 X 16M / Detector: PIXEL / Date: Oct 18, 2018
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Method to determine structure: MOLECULAR REPLACEMENT Starting model: in-house model Resolution: 1.45→42.56 Å / Cor.coef. Fo:Fc: 0.978 / Cor.coef. Fo:Fc free: 0.975 / SU B: 2.219 / SU ML: 0.037 / SU R Cruickshank DPI: 0.058 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.058 / ESU R Free: 0.053 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.1633
2199
5.1 %
RANDOM
Rwork
0.1328
-
-
-
obs
0.1344
40701
99.89 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK
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