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Open data
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Basic information
Entry | Database: PDB / ID: 7q8h | |||||||||
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Title | Peptide EVCKKKK in complex with human cathepsin V C25A mutant | |||||||||
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![]() | HYDROLASE / CathepsinV / Peptidyl substrate | |||||||||
Function / homology | ![]() cathepsin V / RUNX1 regulates transcription of genes involved in differentiation of keratinocytes / Trafficking and processing of endosomal TLR / Assembly of collagen fibrils and other multimeric structures / Activation of Matrix Metalloproteinases / cysteine-type endopeptidase activator activity involved in apoptotic process / extracellular matrix disassembly / cysteine-type peptidase activity / MHC class II antigen presentation / Degradation of the extracellular matrix ...cathepsin V / RUNX1 regulates transcription of genes involved in differentiation of keratinocytes / Trafficking and processing of endosomal TLR / Assembly of collagen fibrils and other multimeric structures / Activation of Matrix Metalloproteinases / cysteine-type endopeptidase activator activity involved in apoptotic process / extracellular matrix disassembly / cysteine-type peptidase activity / MHC class II antigen presentation / Degradation of the extracellular matrix / lysosomal lumen / positive regulation of apoptotic signaling pathway / proteolysis involved in protein catabolic process / Endosomal/Vacuolar pathway / antigen processing and presentation of exogenous peptide antigen via MHC class II / immune response / cysteine-type endopeptidase activity / serine-type endopeptidase activity / extracellular space / extracellular region Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Loboda, J. / Sosnowski, P. / Tusar, L. / Vidmar, R. / Vizovisek, M. / Horvat, J. / Kosec, G. / Impens, F. / Demol, H. / Turk, B. ...Loboda, J. / Sosnowski, P. / Tusar, L. / Vidmar, R. / Vizovisek, M. / Horvat, J. / Kosec, G. / Impens, F. / Demol, H. / Turk, B. / Gevaert, K. / Turk, D. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Proteomic data and structure analysis combined reveal interplay of structural rigidity and flexibility on selectivity of cysteine cathepsins. Authors: Tusar, L. / Loboda, J. / Impens, F. / Sosnowski, P. / Van Quickelberghe, E. / Vidmar, R. / Demol, H. / Sedeyn, K. / Saelens, X. / Vizovisek, M. / Mihelic, M. / Fonovic, M. / Horvat, J. / ...Authors: Tusar, L. / Loboda, J. / Impens, F. / Sosnowski, P. / Van Quickelberghe, E. / Vidmar, R. / Demol, H. / Sedeyn, K. / Saelens, X. / Vizovisek, M. / Mihelic, M. / Fonovic, M. / Horvat, J. / Kosec, G. / Turk, B. / Gevaert, K. / Turk, D. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 200.1 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 7q8dC ![]() 7q8fC ![]() 7q8gC ![]() 7q8iC ![]() 7q8jC ![]() 7q8kC ![]() 7q8lC ![]() 7q8mC ![]() 7q8nC ![]() 7q8oC ![]() 7q8pC ![]() 7q8qC ![]() 7q9cC ![]() 7q9hC ![]() 7qffC ![]() 7qfhC ![]() 7qhjC ![]() 7qhkC ![]() 1fh0S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
-Protein / Protein/peptide , 2 types, 4 molecules AABAPAPB
#1: Protein | Mass: 24021.936 Da / Num. of mol.: 2 / Mutation: C25A, N179Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #2: Protein/peptide | Mass: 890.167 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) ![]() |
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-Non-polymers , 5 types, 432 molecules ![](data/chem/img/MPD.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/TFA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/CL.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/TFA.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-MPD / ( #4: Chemical | ChemComp-CL / | #5: Chemical | #6: Chemical | ChemComp-TFA / #7: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.72 Å3/Da / Density % sol: 54.76 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / Details: 77% MPD, 23 % of 60 mM TRIS, pH 8 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Jan 26, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→50 Å / Num. obs: 56400 / % possible obs: 99.8 % / Redundancy: 10.1 % / CC1/2: 0.998 / Net I/σ(I): 14.06 |
Reflection shell | Resolution: 1.75→1.81 Å / Num. unique obs: 5475 / CC1/2: 0.64 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1FH0 Resolution: 1.75→46.87 Å / Cor.coef. Fo:Fc: 0.9131 / Cor.coef. Fo:Fc free: 0.8845 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 16.5 / Stereochemistry target values: ML
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Solvent computation | Solvent model: MASK FLAT BULK SOLVENT MODEL / Bsol: 34.92 Å2 / ksol: 0.37 e/Å3 | |||||||||||||||||||||||||
Displacement parameters | Biso max: 186.55 Å2 / Biso mean: 27.79 Å2 / Biso min: 10.97 Å2
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Refinement step | Cycle: LAST / Resolution: 1.75→46.87 Å
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LS refinement shell | Resolution: 1.75→1.78 Å / Total num. of bins used: 20
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