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Open data
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Basic information
Entry | Database: PDB / ID: 7pud | ||||||
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Title | Bryoporin - actinoporin from moss Physcomitrium patens | ||||||
![]() | Bryoporin | ||||||
![]() | PLANT PROTEIN / Actinoporin / Membrane-binding / pore-formation / moss | ||||||
Function / homology | Sea anemone actinoporin-like / Sea anemone cytotoxic protein / Cytolysin/lectin / pore complex assembly / hemolysis in another organism / channel activity / pore complex / monoatomic cation transport / Bryoporin![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Solinc, G. / Anderluh, G. / Podobnik, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Pore-forming moss protein bryoporin is structurally and mechanistically related to actinoporins from evolutionarily distant cnidarians. Authors: Solinc, G. / Svigelj, T. / Omersa, N. / Snoj, T. / Pirc, K. / Znidarsic, N. / Yamaji-Hasegawa, A. / Kobayashi, T. / Anderluh, G. / Podobnik, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.1 KB | Display | ![]() |
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PDB format | ![]() | 39 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 442.6 KB | Display | ![]() |
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Full document | ![]() | 442.7 KB | Display | |
Data in XML | ![]() | 11 KB | Display | |
Data in CIF | ![]() | 15.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 3vwiS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21422.822 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||
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#2: Chemical | #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.97 Å3/Da / Density % sol: 37.6 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / Details: ammonium sulphate, NaCl |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Mar 10, 2015 Details: a vertical collimating mirror, a double-crystal Si(111) monochromator, a bendable focussing mirror |
Radiation | Monochromator: a vertical collimating mirror, a double-crystal Si(111) monochromator, a bendable focussing mirror Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.25→34.41 Å / Num. obs: 45262 / % possible obs: 94.78 % / Redundancy: 6 % / Biso Wilson estimate: 9.98 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.04733 / Rpim(I) all: 0.02163 / Rrim(I) all: 0.05227 / Net I/av σ(I): 21.01 / Net I/σ(I): 21.01 |
Reflection shell | Resolution: 1.251→1.295 Å / Redundancy: 4.9 % / Rmerge(I) obs: 0.4168 / Mean I/σ(I) obs: 3.6 / Num. unique obs: 3873 / CC1/2: 0.933 / CC star: 0.982 / Rpim(I) all: 0.2023 / Rrim(I) all: 0.465 / % possible all: 82.39 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 3VWI Resolution: 1.25→34.41 Å / SU ML: 0.1354 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 23.7195 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 14.67 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.25→34.41 Å
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Refine LS restraints |
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LS refinement shell |
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