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- PDB-7pbh: Structural characterization of Thorarchaeota profilin indicates a... -

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Basic information

Entry
Database: PDB / ID: 7pbh
TitleStructural characterization of Thorarchaeota profilin indicates a eukaryotic-like fold but with an extended N-terminus
ComponentsProfilin
KeywordsPROTEIN BINDING / Thor archaea Profilin
Function / homologyProfilin / Profilin / Profilin superfamily / actin binding / Uncharacterized protein
Function and homology information
Biological speciesCandidatus Thorarchaeota (archaea)
MethodSOLUTION NMR / simulated annealing
AuthorsCelestine, C.
Funding support1items
OrganizationGrant numberCountry
Wenner-Gren Foundation
CitationJournal: Adv Biol (Weinh) / Year: 2022
Title: Structural Characterization of a Thorarchaeota Profilin Indicates Eukaryotic-Like Features but with an Extended N-Terminus.
Authors: Inturi, R. / Lara, S. / Derweesh, M. / Chi, C.N.
History
DepositionAug 2, 2021Deposition site: PDBE / Processing site: PDBE
Revision 1.0Aug 10, 2022Provider: repository / Type: Initial release
Revision 1.1Feb 21, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / citation / citation_author
Item: _citation.journal_abbrev / _citation.journal_id_CSD ..._citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: Profilin


Theoretical massNumber of molelcules
Total (without water)15,7841
Polymers15,7841
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: NMR Distance Restraints
TypeNameSymmetry operationNumber
identity operation1_5551
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 100target function
RepresentativeModel #1target function

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Components

#1: Protein Profilin


Mass: 15783.573 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Candidatus Thorarchaeota (archaea) / Gene: EU537_07650 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A524EIQ6

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDSample stateSpectrometer-IDType
111isotropic12D 1H-15N HSQC
121isotropic12D 1H-13C HSQC
131isotropic13D 1H-13C NOESY
141isotropic13D 1H-15N NOESY
151isotropic13D HBHA(CO)NH
161isotropic13D HN(COCA)CB
171isotropic13D HN(CA)CB

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Sample preparation

DetailsType: solution
Contents: 3 mM [U-13C; U-15N] 13C-15N-Thor-profilin, 90% H2O/10% D2O
Label: 13C-15N-Thor-profilin / Solvent system: 90% H2O/10% D2O
SampleConc.: 3 mM / Component: 13C-15N-Thor-profilin / Isotopic labeling: [U-13C; U-15N]
Sample conditionsIonic strength: 50 mM / Label: 13C-15N-Thor-profilin / pH: 6.5 / Pressure: 1 bar / Temperature: 298 K

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NMR measurement

NMR spectrometerType: Bruker AVANCE NEO / Manufacturer: Bruker / Model: AVANCE NEO / Field strength: 600 MHz

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Processing

NMR software
NameDeveloperClassification
CYANAGuntert P.structure calculation
CYANAGuntert, Mumenthaler and Wuthrichrefinement
CcpNmr AnalysisCCPNpeak picking
RefinementMethod: simulated annealing / Software ordinal: 2
NMR representativeSelection criteria: target function
NMR ensembleConformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20

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