Mass: 18635.123 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: First 8 residues are not shown and correspond to sequence MKHHHHHH Source: (gene. exp.) Chikungunya virus (strain S27-African prototype) Strain: S27-African prototype / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta 2 / References: UniProt: Q8JUX6
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC
1
2
2
isotropic
1
2D 1H-13C HSQC
1
3
2
isotropic
1
3DHBHA(CO)NH
1
4
1
isotropic
1
3D HNHA
1
5
2
isotropic
1
3DCBCA(CO)NH
1
9
2
isotropic
1
3D HN(CA)CB
1
8
2
isotropic
1
3D HNCA
1
7
2
isotropic
1
3DHN(CO)CA
1
6
2
isotropic
1
3D HNCO
1
11
2
isotropic
1
3DHN(CA)CO
1
10
2
isotropic
1
3D (H)CCH-TOCSY
1
12
2
isotropic
1
3D 1H-13C NOESY aliphatic
1
13
2
isotropic
1
3D 1H-13C NOESY aromatic
1
14
1
isotropic
1
3D 1H-15N NOESY
1
15
3
isotropic
1
2D 1H-1H NOESY
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Sample preparation
Details
Type
Solution-ID
Contents
Details
Label
Solvent system
solution
1
0.6 mM [U-99% 15N] Chikungunya virus macro domain, 20 mM no sodium chloride, 10 mM no HEPES, 2 mM no DTT, 2 mM no EDTA, 0.25 mM no DSS, 10 % no D2O, 90% H2O/10% D2O
0.6 mM [U-99% 13C; U-99% 15N] Chikungunya virus macro domain, 20 mM no sodium chloride, 10 mM no HEPES, 2 mM no DTT, 2 mM no EDTA, 0.25 mM no DSS, 10 % no D2O, 90% H2O/10% D2O