根拠: Likely to be dimeric based on gel-filtration and crystallographic studies of related short-chain TDHs and PISA analysis of buried surface area and contacts (Krissinel, E. & Henrick, K. (2007). ...根拠: Likely to be dimeric based on gel-filtration and crystallographic studies of related short-chain TDHs and PISA analysis of buried surface area and contacts (Krissinel, E. & Henrick, K. (2007). J. Molec. Biol. 372, 774-797).
温度: 293 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 8.5 詳細: 5 microlitres of enzyme at a concentration of 5 mg/ml and 5 microlitres of 0.2 M lithium sulphate, 0.1 M Tris-HCl pH 8.5, 30 % v/v PEG 4000 (Molecular Dimensions Limited Structure Screen I, condition 35). Temp details: Ambient temperature
解像度: 2.6→63.28 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.939 / SU B: 10.429 / SU ML: 0.198 / SU R Cruickshank DPI: 0.234 / 交差検証法: FREE R-VALUE / σ(F): 0 / ESU R: 0.234 / ESU R Free: 0.21 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
Rfactor
反射数
%反射
Selection details
Rfree
0.2389
2500
5 %
RANDOM
Rwork
0.2004
-
-
-
obs
0.2024
47443
97.28 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK